1oe1

From Proteopedia

(Difference between revisions)

Revision as of 17:12, 15 December 2021

Atomic Resolution Structure of the Wildtype Native Nitrite Reductase from Alcaligenes xylosoxidans

Structural highlights

1oe1 is a 1 chain structure with sequence from Alcaligenes xylosoxidans. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Ligands:	,
NonStd Res:
Related:	1bq5, 1gs6, 1gs7, 1gs8, 1hau, 1haw, 1ndt, 1oe2
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Evolutionary Conservation

Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.

Publication Abstract from PubMed

We provide the first atomic resolution (<1.20 A) structure of a copper protein, nitrite reductase, and of a mutant of the catalytically important Asp92 residue (D92E). The atomic resolution where carbon-carbon bonds of the peptide become clearly resolved, remains a key goal of structural analysis. Despite much effort and technological progress, still very few structures are known at such resolution. For example, in the Protein Data Bank (PDB) there are some 200 structures of copper proteins but the highest resolution structure is that of amicyanin, a small (12 kDa) protein, which has been resolved to 1.30 A. Here, we present the structures of wild-type copper nitrite reductase (wtNiR) from Alcaligenes xylosoxidans (36.5 kDa monomer), the "half-apo" recombinant native protein and the D92E mutant at 1.04, 1.15 and 1.12A resolutions, respectively. These structures provide the basis from which to build a detailed mechanism of this important enzyme.

Atomic resolution structures of native copper nitrite reductase from Alcaligenes xylosoxidans and the active site mutant Asp92Glu.,Ellis MJ, Dodd FE, Sawers G, Eady RR, Hasnain SS J Mol Biol. 2003 Apr 25;328(2):429-38. PMID:12691751^[1]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Ellis MJ, Dodd FE, Sawers G, Eady RR, Hasnain SS. Atomic resolution structures of native copper nitrite reductase from Alcaligenes xylosoxidans and the active site mutant Asp92Glu. J Mol Biol. 2003 Apr 25;328(2):429-38. PMID:12691751

1 Structural highlights
2 Evolutionary Conservation
3 Publication Abstract from PubMed
4 See Also
5 References

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OCA

Retrieved from "http://52.214.119.220/wiki/index.php/1oe1"

@@ Line 3: / Line 3: @@
 <StructureSection load='1oe1' size='340' side='right'caption='[[1oe1]], [[Resolution|resolution]] 1.04&Aring;' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[1oe1]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Alcaligenes_xylosoxidans Alcaligenes xylosoxidans]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1OE1 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1OE1 FirstGlance]. <br>
+<table><tr><td colspan='2'>[[1oe1]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Alcaligenes_xylosoxidans Alcaligenes xylosoxidans]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1OE1 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1OE1 FirstGlance]. <br>
-</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CU:COPPER+(II)+ION'>CU</scene>, <scene name='pdbligand=PG4:TETRAETHYLENE+GLYCOL'>PG4</scene></td></tr>
+</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CU:COPPER+(II)+ION'>CU</scene>, <scene name='pdbligand=PG4:TETRAETHYLENE+GLYCOL'>PG4</scene></td></tr>
 <tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=PCA:PYROGLUTAMIC+ACID'>PCA</scene></td></tr>
-<tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[1bq5|1bq5]], [[1gs6|1gs6]], [[1gs7|1gs7]], [[1gs8|1gs8]], [[1hau|1hau]], [[1haw|1haw]], [[1ndt|1ndt]], [[1oe2|1oe2]]</td></tr>
+<tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[1bq5|1bq5]], [[1gs6|1gs6]], [[1gs7|1gs7]], [[1gs8|1gs8]], [[1hau|1hau]], [[1haw|1haw]], [[1ndt|1ndt]], [[1oe2|1oe2]]</div></td></tr>
-<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1oe1 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1oe1 OCA], [http://pdbe.org/1oe1 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1oe1 RCSB], [http://www.ebi.ac.uk/pdbsum/1oe1 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=1oe1 ProSAT]</span></td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1oe1 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1oe1 OCA], [https://pdbe.org/1oe1 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1oe1 RCSB], [https://www.ebi.ac.uk/pdbsum/1oe1 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1oe1 ProSAT]</span></td></tr>
 </table>
 == Evolutionary Conservation ==

1oe1

From Proteopedia

Revision as of 17:12, 15 December 2021

Atomic Resolution Structure of the Wildtype Native Nitrite Reductase from Alcaligenes xylosoxidans

Structural highlights

Evolutionary Conservation

Publication Abstract from PubMed

See Also

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

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