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2dzn

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Revision as of 17:19, 15 December 2021

Crystal structure analysis of yeast Nas6p complexed with the proteasome subunit, rpt3

Structural highlights

2dzn is a 6 chain structure with sequence from Atcc 18824. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Related:	2dzo
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT, TOPSAN

Function

[PSD10_YEAST] Acts as a chaperone during the assembly of the 26S proteasome, specifically of the 19S regulatory complex (RC) and appears to have an overlapping role with RPN14.^[1] ^[2] ^[3] [PRS6B_YEAST] The 26S protease is involved in the ATP-dependent degradation of ubiquitinated proteins. The regulatory (or ATPase) complex confers ATP dependency and substrate specificity to the 26S complex (By similarity).

Evolutionary Conservation

Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.

Publication Abstract from PubMed

The 26S proteasome-dependent protein degradation is an evolutionarily conserved process. The mammalian oncoprotein gankyrin, which associates with S6 of the proteasome, facilitates the degradation of pRb, and thus possibly acts as a bridging factor between the proteasome and its substrates. However, the mechanism of the proteasome-dependent protein degradation in yeast is poorly understood. Here, we report the tertiary structure of the complex between Nas6 and a C-terminal domain of Rpt3, which are the yeast orthologues of gankyrin and S6, respectively. The concave region of Nas6 bound to the alpha-helical domain of Rpt3. The stable interaction between Nas6 and Rpt3 was mediated by intermolecular interactions composed of complementary charged patches. The recognition of Rpt3 by Nas6 in the crystal suggests that Nas6 is indeed a subunit of the 26S proteasome. These results provide a structural basis for the association between Nas6 and the heterohexameric ATPase ring of the proteasome through Rpt3.

Structural basis for the recognition between the regulatory particles Nas6 and Rpt3 of the yeast 26S proteasome.,Nakamura Y, Umehara T, Tanaka A, Horikoshi M, Padmanabhan B, Yokoyama S Biochem Biophys Res Commun. 2007 Aug 3;359(3):503-9. Epub 2007 May 29. PMID:17555716^[4]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Roelofs J, Park S, Haas W, Tian G, McAllister FE, Huo Y, Lee BH, Zhang F, Shi Y, Gygi SP, Finley D. Chaperone-mediated pathway of proteasome regulatory particle assembly. Nature. 2009 Jun 11;459(7248):861-5. PMID:19412159 doi:nature08063
↑ Funakoshi M, Tomko RJ Jr, Kobayashi H, Hochstrasser M. Multiple assembly chaperones govern biogenesis of the proteasome regulatory particle base. Cell. 2009 May 29;137(5):887-99. Epub 2009 May 14. PMID:19446322 doi:S0092-8674(09)00526-1
↑ Hori T, Kato S, Saeki M, DeMartino GN, Slaughter CA, Takeuchi J, Toh-e A, Tanaka K. cDNA cloning and functional analysis of p28 (Nas6p) and p40.5 (Nas7p), two novel regulatory subunits of the 26S proteasome. Gene. 1998 Aug 17;216(1):113-22. PMID:9714768
↑ Nakamura Y, Umehara T, Tanaka A, Horikoshi M, Padmanabhan B, Yokoyama S. Structural basis for the recognition between the regulatory particles Nas6 and Rpt3 of the yeast 26S proteasome. Biochem Biophys Res Commun. 2007 Aug 3;359(3):503-9. Epub 2007 May 29. PMID:17555716 doi:10.1016/j.bbrc.2007.05.138

1 Structural highlights
2 Function
3 Evolutionary Conservation
4 Publication Abstract from PubMed
5 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/2dzn"

@@ Line 3: / Line 3: @@
 <StructureSection load='2dzn' size='340' side='right'caption='[[2dzn]], [[Resolution|resolution]] 2.20&Aring;' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[2dzn]] is a 6 chain structure with sequence from [http://en.wikipedia.org/wiki/Atcc_18824 Atcc 18824]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2DZN OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2DZN FirstGlance]. <br>
+<table><tr><td colspan='2'>[[2dzn]] is a 6 chain structure with sequence from [https://en.wikipedia.org/wiki/Atcc_18824 Atcc 18824]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2DZN OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2DZN FirstGlance]. <br>
-</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[2dzo|2dzo]]</td></tr>
+</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[2dzo|2dzo]]</div></td></tr>
-<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2dzn FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2dzn OCA], [http://pdbe.org/2dzn PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2dzn RCSB], [http://www.ebi.ac.uk/pdbsum/2dzn PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=2dzn ProSAT], [http://www.topsan.org/Proteins/RSGI/2dzn TOPSAN]</span></td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2dzn FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2dzn OCA], [https://pdbe.org/2dzn PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2dzn RCSB], [https://www.ebi.ac.uk/pdbsum/2dzn PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2dzn ProSAT], [https://www.topsan.org/Proteins/RSGI/2dzn TOPSAN]</span></td></tr>
 </table>
 == Function ==
-[[http://www.uniprot.org/uniprot/PSD10_YEAST PSD10_YEAST]] Acts as a chaperone during the assembly of the 26S proteasome, specifically of the 19S regulatory complex (RC) and appears to have an overlapping role with RPN14.<ref>PMID:19412159</ref> <ref>PMID:19446322</ref> <ref>PMID:9714768</ref>  [[http://www.uniprot.org/uniprot/PRS6B_YEAST PRS6B_YEAST]] The 26S protease is involved in the ATP-dependent degradation of ubiquitinated proteins. The regulatory (or ATPase) complex confers ATP dependency and substrate specificity to the 26S complex (By similarity).
+[[https://www.uniprot.org/uniprot/PSD10_YEAST PSD10_YEAST]] Acts as a chaperone during the assembly of the 26S proteasome, specifically of the 19S regulatory complex (RC) and appears to have an overlapping role with RPN14.<ref>PMID:19412159</ref> <ref>PMID:19446322</ref> <ref>PMID:9714768</ref>  [[https://www.uniprot.org/uniprot/PRS6B_YEAST PRS6B_YEAST]] The 26S protease is involved in the ATP-dependent degradation of ubiquitinated proteins. The regulatory (or ATPase) complex confers ATP dependency and substrate specificity to the 26S complex (By similarity).
 == Evolutionary Conservation ==
 [[Image:Consurf_key_small.gif|200px|right]]

2dzn

From Proteopedia

Revision as of 17:19, 15 December 2021

Crystal structure analysis of yeast Nas6p complexed with the proteasome subunit, rpt3

Structural highlights

Function

Evolutionary Conservation

Publication Abstract from PubMed

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

Views

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