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| | ==Crystal structure of the Mus81-Eme1 complex== | | ==Crystal structure of the Mus81-Eme1 complex== |
| - | <StructureSection load='2ziu' size='340' side='right' caption='[[2ziu]], [[Resolution|resolution]] 2.70Å' scene=''> | + | <StructureSection load='2ziu' size='340' side='right'caption='[[2ziu]], [[Resolution|resolution]] 2.70Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[2ziu]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Brachidanio_rerio Brachidanio rerio] and [http://en.wikipedia.org/wiki/Human Human]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2ZIU OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2ZIU FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[2ziu]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Brachidanio_rerio Brachidanio rerio] and [https://en.wikipedia.org/wiki/Human Human]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2ZIU OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2ZIU FirstGlance]. <br> |
| - | </td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[2ziv|2ziv]], [[2ziw|2ziw]], [[2zix|2zix]]</td></tr> | + | </td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[2ziv|2ziv]], [[2ziw|2ziw]], [[2zix|2zix]]</div></td></tr> |
| - | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">mus81 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=7955 Brachidanio rerio]), EME1 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN])</td></tr> | + | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">mus81 ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=7955 Brachidanio rerio]), EME1 ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN])</td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2ziu FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2ziu OCA], [http://pdbe.org/2ziu PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2ziu RCSB], [http://www.ebi.ac.uk/pdbsum/2ziu PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=2ziu ProSAT]</span></td></tr> | + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2ziu FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2ziu OCA], [https://pdbe.org/2ziu PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2ziu RCSB], [https://www.ebi.ac.uk/pdbsum/2ziu PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2ziu ProSAT]</span></td></tr> |
| | </table> | | </table> |
| | == Function == | | == Function == |
| - | [[http://www.uniprot.org/uniprot/EME1_HUMAN EME1_HUMAN]] Interacts with MUS81 to form a DNA structure-specific endonuclease with substrate preference for branched DNA structures with a 5'-end at the branch nick. Typical substrates include 3'-flap structures, replication forks and nicked Holliday junctions. May be required in mitosis for the processing of stalled or collapsed replication forks.<ref>PMID:12686547</ref> <ref>PMID:12721304</ref> <ref>PMID:14617801</ref> <ref>PMID:17289582</ref> | + | [[https://www.uniprot.org/uniprot/EME1_HUMAN EME1_HUMAN]] Interacts with MUS81 to form a DNA structure-specific endonuclease with substrate preference for branched DNA structures with a 5'-end at the branch nick. Typical substrates include 3'-flap structures, replication forks and nicked Holliday junctions. May be required in mitosis for the processing of stalled or collapsed replication forks.<ref>PMID:12686547</ref> <ref>PMID:12721304</ref> <ref>PMID:14617801</ref> <ref>PMID:17289582</ref> |
| | == Evolutionary Conservation == | | == Evolutionary Conservation == |
| | [[Image:Consurf_key_small.gif|200px|right]] | | [[Image:Consurf_key_small.gif|200px|right]] |
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| | ==See Also== | | ==See Also== |
| - | *[[Endonuclease|Endonuclease]] | + | *[[Endonuclease 3D structures|Endonuclease 3D structures]] |
| | == References == | | == References == |
| | <references/> | | <references/> |
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| | [[Category: Brachidanio rerio]] | | [[Category: Brachidanio rerio]] |
| | [[Category: Human]] | | [[Category: Human]] |
| | + | [[Category: Large Structures]] |
| | [[Category: Chang, J H]] | | [[Category: Chang, J H]] |
| | [[Category: Cho, Y]] | | [[Category: Cho, Y]] |
| Structural highlights
Function
[EME1_HUMAN] Interacts with MUS81 to form a DNA structure-specific endonuclease with substrate preference for branched DNA structures with a 5'-end at the branch nick. Typical substrates include 3'-flap structures, replication forks and nicked Holliday junctions. May be required in mitosis for the processing of stalled or collapsed replication forks.[1] [2] [3] [4]
Evolutionary Conservation
Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.
Publication Abstract from PubMed
The Mus81-Eme1 complex is a structure-specific endonuclease that plays an important role in rescuing stalled replication forks and resolving the meiotic recombination intermediates in eukaryotes. We have determined the crystal structure of the Mus81-Eme1 complex. Both Mus81 and Eme1 consist of a central nuclease domain, two repeats of the helix-hairpin-helix (HhH) motif at their C-terminal region, and a linker helix. While each domain structure resembles archaeal XPF homologs, the overall structure is significantly different from those due to the structure of a linker helix. We show that a flexible intradomain linker that formed with 36 residues in the nuclease domain of Eme1 is essential for the recognition of DNA. We identified several basic residues lining the outer surface of the active site cleft of Mus81 that are involved in the interaction with a flexible arm of a nicked Holliday junction (HJ). These interactions might contribute to the optimal positioning of the opposite junction across the nick into the catalytic site, which provided the basis for the "nick and counternick" mechanism of Mus81-Eme1 and for the nicked HJ to be the favored in vitro substrate of this enzyme.
Crystal structure of the Mus81-Eme1 complex.,Chang JH, Kim JJ, Choi JM, Lee JH, Cho Y Genes Dev. 2008 Apr 15;22(8):1093-106. PMID:18413719[5]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
See Also
References
- ↑ Ogrunc M, Sancar A. Identification and characterization of human MUS81-MMS4 structure-specific endonuclease. J Biol Chem. 2003 Jun 13;278(24):21715-20. Epub 2003 Apr 9. PMID:12686547 doi:http://dx.doi.org/10.1074/jbc.M302484200
- ↑ Ciccia A, Constantinou A, West SC. Identification and characterization of the human mus81-eme1 endonuclease. J Biol Chem. 2003 Jul 4;278(27):25172-8. Epub 2003 Apr 29. PMID:12721304 doi:http://dx.doi.org/10.1074/jbc.M302882200
- ↑ Blais V, Gao H, Elwell CA, Boddy MN, Gaillard PH, Russell P, McGowan CH. RNA interference inhibition of Mus81 reduces mitotic recombination in human cells. Mol Biol Cell. 2004 Feb;15(2):552-62. Epub 2003 Nov 14. PMID:14617801 doi:http://dx.doi.org/10.1091/mbc.E03-08-0580
- ↑ Ciccia A, Ling C, Coulthard R, Yan Z, Xue Y, Meetei AR, Laghmani el H, Joenje H, McDonald N, de Winter JP, Wang W, West SC. Identification of FAAP24, a Fanconi anemia core complex protein that interacts with FANCM. Mol Cell. 2007 Feb 9;25(3):331-43. PMID:17289582 doi:http://dx.doi.org/S1097-2765(07)00007-X
- ↑ Chang JH, Kim JJ, Choi JM, Lee JH, Cho Y. Crystal structure of the Mus81-Eme1 complex. Genes Dev. 2008 Apr 15;22(8):1093-106. PMID:18413719 doi:22/8/1093
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