6zsm
From Proteopedia
(Difference between revisions)
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==Crystal structure of rsGCaMP double mutant Ile80His/Val116Ile in the ON state (non-illuminated)== | ==Crystal structure of rsGCaMP double mutant Ile80His/Val116Ile in the ON state (non-illuminated)== | ||
| - | <StructureSection load='6zsm' size='340' side='right'caption='[[6zsm]]' scene=''> | + | <StructureSection load='6zsm' size='340' side='right'caption='[[6zsm]], [[Resolution|resolution]] 1.95Å' scene=''> |
== Structural highlights == | == Structural highlights == | ||
| - | <table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6ZSM OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6ZSM FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[6zsm]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6ZSM OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6ZSM FirstGlance]. <br> |
| - | </td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6zsm FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6zsm OCA], [https://pdbe.org/6zsm PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6zsm RCSB], [https://www.ebi.ac.uk/pdbsum/6zsm PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6zsm ProSAT]</span></td></tr> | + | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=FMT:FORMIC+ACID'>FMT</scene></td></tr> |
| + | <tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=PIA:[(4Z)-2-[(1S)-1-AMINOETHYL]-4-(4-HYDROXYBENZYLIDENE)-5-OXO-4,5-DIHYDRO-1H-IMIDAZOL-1-YL]ACETIC+ACID'>PIA</scene></td></tr> | ||
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6zsm FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6zsm OCA], [https://pdbe.org/6zsm PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6zsm RCSB], [https://www.ebi.ac.uk/pdbsum/6zsm PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6zsm ProSAT]</span></td></tr> | ||
</table> | </table> | ||
| + | == Function == | ||
| + | [[https://www.uniprot.org/uniprot/GFP_AEQVI GFP_AEQVI]] Energy-transfer acceptor. Its role is to transduce the blue chemiluminescence of the protein aequorin into green fluorescent light by energy transfer. Fluoresces in vivo upon receiving energy from the Ca(2+)-activated photoprotein aequorin. | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | Reversibly photo-switchable proteins are essential for many super-resolution fluorescence microscopic and optoacoustic imaging methods. However, they have yet to be used as sensors that measure the distribution of specific analytes at the nanoscale or in the tissues of live animals. Here we constructed the prototype of a photo-switchable Ca(2+) sensor based on GCaMP5G that can be switched with 405/488-nm light and describe its molecular mechanisms at the structural level, including the importance of the interaction of the core barrel structure of the fluorescent protein with the Ca(2+) receptor moiety. We demonstrate super-resolution imaging of Ca(2+) concentration in cultured cells and optoacoustic Ca(2+) imaging in implanted tumor cells in mice under controlled Ca(2+) conditions. Finally, we show the generalizability of the concept by constructing examples of photo-switching maltose and dopamine sensors based on periplasmatic binding protein and G-protein-coupled receptor-based sensors. | ||
| + | |||
| + | Genetically encoded photo-switchable molecular sensors for optoacoustic and super-resolution imaging.,Mishra K, Fuenzalida-Werner JP, Pennacchietti F, Janowski R, Chmyrov A, Huang Y, Zakian C, Klemm U, Testa I, Niessing D, Ntziachristos V, Stiel AC Nat Biotechnol. 2021 Nov 29. pii: 10.1038/s41587-021-01100-5. doi:, 10.1038/s41587-021-01100-5. PMID:34845372<ref>PMID:34845372</ref> | ||
| + | |||
| + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | ||
| + | </div> | ||
| + | <div class="pdbe-citations 6zsm" style="background-color:#fffaf0;"></div> | ||
| + | == References == | ||
| + | <references/> | ||
__TOC__ | __TOC__ | ||
</StructureSection> | </StructureSection> | ||
[[Category: Large Structures]] | [[Category: Large Structures]] | ||
| - | [[Category: Fuenzalida-Werner | + | [[Category: Fuenzalida-Werner, J P]] |
| - | [[Category: Janowski R]] | + | [[Category: Janowski, R]] |
| - | [[Category: Mishra K]] | + | [[Category: Mishra, K]] |
| - | [[Category: Niessing D]] | + | [[Category: Niessing, D]] |
| - | [[Category: Stiel | + | [[Category: Stiel, A C]] |
| + | [[Category: Calcium sensor]] | ||
| + | [[Category: Calmodulin]] | ||
| + | [[Category: Fluorescent protein]] | ||
| + | [[Category: Reversible switchable fluorescent protein]] | ||
Revision as of 15:33, 22 December 2021
Crystal structure of rsGCaMP double mutant Ile80His/Val116Ile in the ON state (non-illuminated)
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