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| | ==Crystal Structure of Cytochrome c555 from Aquifex aeolicus== | | ==Crystal Structure of Cytochrome c555 from Aquifex aeolicus== |
| - | <StructureSection load='2zxy' size='340' side='right' caption='[[2zxy]], [[Resolution|resolution]] 1.15Å' scene=''> | + | <StructureSection load='2zxy' size='340' side='right'caption='[[2zxy]], [[Resolution|resolution]] 1.15Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[2zxy]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/"aquifex_aeolicus"_huber_and_stetter_2001 "aquifex aeolicus" huber and stetter 2001]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2ZXY OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2ZXY FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[2zxy]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/"aquifex_aeolicus"_huber_and_stetter_2001 "aquifex aeolicus" huber and stetter 2001]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2ZXY OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2ZXY FirstGlance]. <br> |
| - | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=HEC:HEME+C'>HEC</scene></td></tr> | + | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=HEC:HEME+C'>HEC</scene></td></tr> |
| - | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">cycB2, aq_1550 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=63363 "Aquifex aeolicus" Huber and Stetter 2001])</td></tr> | + | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">cycB2, aq_1550 ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=63363 "Aquifex aeolicus" Huber and Stetter 2001])</td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2zxy FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2zxy OCA], [http://pdbe.org/2zxy PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2zxy RCSB], [http://www.ebi.ac.uk/pdbsum/2zxy PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=2zxy ProSAT]</span></td></tr> | + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2zxy FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2zxy OCA], [https://pdbe.org/2zxy PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2zxy RCSB], [https://www.ebi.ac.uk/pdbsum/2zxy PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2zxy ProSAT]</span></td></tr> |
| | </table> | | </table> |
| | == Evolutionary Conservation == | | == Evolutionary Conservation == |
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| | ==See Also== | | ==See Also== |
| - | *[[Cytochrome c|Cytochrome c]] | + | *[[Cytochrome C 3D structures|Cytochrome C 3D structures]] |
| | == References == | | == References == |
| | <references/> | | <references/> |
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| | </StructureSection> | | </StructureSection> |
| | [[Category: Aquifex aeolicus huber and stetter 2001]] | | [[Category: Aquifex aeolicus huber and stetter 2001]] |
| | + | [[Category: Large Structures]] |
| | [[Category: Kawahara, K]] | | [[Category: Kawahara, K]] |
| | [[Category: Kobayashi, Y]] | | [[Category: Kobayashi, Y]] |
| Structural highlights
Evolutionary Conservation
Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.
Publication Abstract from PubMed
In order to elucidate the relationship between the stability and the structure of the monohaem cytochrome c(555) (AA c(555)) from the hyperthermophilic bacterium Aquifex aeolicus, chemical denaturation and crystal structure determination were carried out. AA c(555) exhibited higher stability than the thermophilic Hydrogenobacter thermophilus cytochrome c(552) (HT c(552)), which is one of the most stable cytochromes c. The three-dimensional crystal structure of AA c(555), which was determined using the multiple anomalous dispersion technique at 1.15 A resolution, included a unique 14-residue extra helix, while the side-chain interactions of several amino-acid residues responsible for the stability of HT c(552) were conserved in AA c(555). The side chain of the Met61 residue in the extra helix was aligned towards the haem, forming a coordination bond between the Met S and haem Fe atoms. In other cytochromes c the corresponding regions always form Omega loops which also include the haem-liganding Met residue and are known to be involved in the initial step in cytochrome c denaturation. The formation of the extra helix in AA c(555) results in the highest helix content, 59.8%, among the monohaem cytochromes c. The extra helix should mainly contribute to the hyperstability of AA c(555) and is presumed to be a novel strategy of cytochromes c for adaptation to a hyperthermophilic environment.
Hyperstability and crystal structure of cytochrome c(555) from hyperthermophilic Aquifex aeolicus.,Obuchi M, Kawahara K, Motooka D, Nakamura S, Yamanaka M, Takeda T, Uchiyama S, Kobayashi Y, Ohkubo T, Sambongi Y Acta Crystallogr D Biol Crystallogr. 2009 Aug;65(Pt 8):804-13. Epub 2009, Jul 17. PMID:19622864[1]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
See Also
References
- ↑ Obuchi M, Kawahara K, Motooka D, Nakamura S, Yamanaka M, Takeda T, Uchiyama S, Kobayashi Y, Ohkubo T, Sambongi Y. Hyperstability and crystal structure of cytochrome c(555) from hyperthermophilic Aquifex aeolicus. Acta Crystallogr D Biol Crystallogr. 2009 Aug;65(Pt 8):804-13. Epub 2009, Jul 17. PMID:19622864 doi:10.1107/S0907444909017314
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