1g1i
From Proteopedia
| Line 1: | Line 1: | ||
[[Image:1g1i.gif|left|200px]] | [[Image:1g1i.gif|left|200px]] | ||
| - | + | <!-- | |
| - | + | The line below this paragraph, containing "STRUCTURE_1g1i", creates the "Structure Box" on the page. | |
| - | + | You may change the PDB parameter (which sets the PDB file loaded into the applet) | |
| - | + | or the SCENE parameter (which sets the initial scene displayed when the page is loaded), | |
| - | + | or leave the SCENE parameter empty for the default display. | |
| - | + | --> | |
| - | | | + | {{STRUCTURE_1g1i| PDB=1g1i | SCENE= }} |
| - | | | + | |
| - | + | ||
| - | }} | + | |
'''CRYSTAL STRUCTURE OF THE OLIGOMERIZATION DOMAIN FROM ROTAVIRUS NSP4''' | '''CRYSTAL STRUCTURE OF THE OLIGOMERIZATION DOMAIN FROM ROTAVIRUS NSP4''' | ||
| Line 19: | Line 16: | ||
==About this Structure== | ==About this Structure== | ||
| - | 1G1I is a [[Single protein]] structure | + | 1G1I is a [[Single protein]] structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1G1I OCA]. |
==Reference== | ==Reference== | ||
| Line 27: | Line 24: | ||
[[Category: Nodelman, I M.]] | [[Category: Nodelman, I M.]] | ||
[[Category: Schutt, C E.]] | [[Category: Schutt, C E.]] | ||
| - | [[Category: | + | [[Category: Homo-tetramer]] |
| - | [[Category: | + | [[Category: Metal binding site]] |
| - | [[Category: | + | [[Category: Ns28]] |
| - | [[Category: | + | [[Category: Parallel coiled-coil]] |
| - | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May 2 17:01:06 2008'' | |
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | |
Revision as of 14:01, 2 May 2008
CRYSTAL STRUCTURE OF THE OLIGOMERIZATION DOMAIN FROM ROTAVIRUS NSP4
Overview
During the maturation of rotaviral particles, non-structural protein 4 (NSP4) plays a critical role in the translocation of the immature capsid into the lumen of the endoplasmic reticulum. Full-length NSP4 and a 22 amino acid peptide (NSP4(114-135)) derived from this protein have been shown to induce diarrhea in young mice in an age-dependent manner, and may therefore be the agent responsible for rotavirally-induced symptoms. We have determined the crystal structure of the oligomerization domain of NSP4 which spans residues 95 to 137 (NSP4(95-137)). NSP4(95-137) self-associates into a parallel, tetrameric coiled-coil, with the hydrophobic core interrupted by three polar layers occupying a and d-heptad positions. Side-chains from two consecutive polar layers, consisting of four Gln123 and two of the four Glu120 residues, coordinate a divalent cation. Two independent structures built from MAD-phased data indicated the presence of a strontium and calcium ion bound at this site, respectively. This metal-binding site appears to play an important role in stabilizing the homo-tetramer, which has implications for the engagement of NSP4 as an enterotoxin.
About this Structure
1G1I is a Single protein structure. Full crystallographic information is available from OCA.
Reference
Crystal structure of the oligomerization domain of NSP4 from rotavirus reveals a core metal-binding site., Bowman GD, Nodelman IM, Levy O, Lin SL, Tian P, Zamb TJ, Udem SA, Venkataraghavan B, Schutt CE, J Mol Biol. 2000 Dec 15;304(5):861-71. PMID:11124032 Page seeded by OCA on Fri May 2 17:01:06 2008
