1g1z
From Proteopedia
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'''NMR Solution Structures of delta-Conotoxin EVIA from Conus ermineus that Selectively Acts on Vertebrate Neuronal Na+ Channels, LEU12-PRO13 Cis isomer''' | '''NMR Solution Structures of delta-Conotoxin EVIA from Conus ermineus that Selectively Acts on Vertebrate Neuronal Na+ Channels, LEU12-PRO13 Cis isomer''' | ||
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==About this Structure== | ==About this Structure== | ||
| - | 1G1Z is a [[Single protein]] structure | + | 1G1Z is a [[Single protein]] structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1G1Z OCA]. |
==Reference== | ==Reference== | ||
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[[Category: Menez, A.]] | [[Category: Menez, A.]] | ||
[[Category: Volpon, L.]] | [[Category: Volpon, L.]] | ||
| - | [[Category: | + | [[Category: Cis/trans isomerism of leu12-pro13 peptide bond]] |
| - | [[Category: | + | [[Category: Hydroxyproline]] |
| - | [[Category: | + | [[Category: Three disulfide linkage]] |
| - | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May 2 17:02:05 2008'' | |
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Revision as of 14:02, 2 May 2008
NMR Solution Structures of delta-Conotoxin EVIA from Conus ermineus that Selectively Acts on Vertebrate Neuronal Na+ Channels, LEU12-PRO13 Cis isomer
Overview
Delta-conotoxin EVIA, from Conus ermineus, is a 32-residue polypeptide cross-linked by three disulfide bonds forming a four-loop framework. delta-Conotoxin EVIA is the first conotoxin known to inhibit sodium channel inactivation in neuronal membranes from amphibians and mammals (subtypes rNa(v)1.2a, rNa(v)1.3, and rNa(v)1.6), without affecting rat skeletal muscle (subtype rNa(v)1.4) and human cardiac muscle (subtype hNa(v)1.5) sodium channel (Barbier, J., Lamthanh, H., Le Gall, F., Favreau, P., Benoit, E., Chen, H., Gilles, N., Ilan, N., Heinemann, S. F., Gordon, D., Menez, A., and Molgo, J. (2004) J. Biol. Chem. 279, 4680-4685). Its structure was solved by NMR and is characterized by a 1:1 cis/trans isomerism of the Leu(12)-Pro(13) peptide bond in slow exchange on the NMR time scale. The structure of both cis and trans isomers could be calculated separately. The isomerism occurs within a specific long disordered loop 2, including residues 11-19. These contribute to an important hydrophobic patch on the surface of the toxin. The rest of the structure matches the "inhibitor cystine-knot motif" of conotoxins from the "O superfamily" with a high structural order. To probe a possible functional role of the Leu(12)-Pro(13) cis/trans isomerism, a Pro(13) --> Ala delta-conotoxin EVIA was synthesized and shown to exist only as a trans isomer. P13A delta-conotoxin EVIA was estimated only two times less active than the wild-type EVIA in binding competition to rat brain synaptosomes and when injected intracerebroventricularly into mice.
About this Structure
1G1Z is a Single protein structure. Full crystallographic information is available from OCA.
Reference
NMR solution structures of delta-conotoxin EVIA from Conus ermineus that selectively acts on vertebrate neuronal Na+ channels., Volpon L, Lamthanh H, Barbier J, Gilles N, Molgo J, Menez A, Lancelin JM, J Biol Chem. 2004 May 14;279(20):21356-66. Epub 2004 Feb 19. PMID:14976206 Page seeded by OCA on Fri May 2 17:02:05 2008
