1g2r
From Proteopedia
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'''Structure of Cytosolic Protein of Unknown Function Coded by Gene from NUSA/INFB Region, a YlxR Homologue''' | '''Structure of Cytosolic Protein of Unknown Function Coded by Gene from NUSA/INFB Region, a YlxR Homologue''' | ||
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[[Category: Maj, L.]] | [[Category: Maj, L.]] | ||
[[Category: Osipiuk, J.]] | [[Category: Osipiuk, J.]] | ||
| - | [[Category: | + | [[Category: Hypothetical]] |
| - | [[Category: | + | [[Category: Mcsg]] |
| - | [[Category: | + | [[Category: Midwest center for structural genomic]] |
| - | [[Category: | + | [[Category: Nusa-infb operon]] |
| - | [[Category: | + | [[Category: Protein structure initiative]] |
| - | [[Category: | + | [[Category: Psi]] |
| - | [[Category: | + | [[Category: Streptococcus pneumoniae]] |
| - | [[Category: | + | [[Category: Structural genomic]] |
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Revision as of 14:03, 2 May 2008
Structure of Cytosolic Protein of Unknown Function Coded by Gene from NUSA/INFB Region, a YlxR Homologue
Overview
The structure of the YlxR protein of unknown function from Streptococcus pneumonia was determined to 1.35 A. YlxR is expressed from the nusA/infB operon in bacteria and belongs to a small protein family (COG2740) that shares a conserved sequence motif GRGA(Y/W). The family shows no significant amino-acid sequence similarity with other proteins. Three-wavelength diffraction MAD data were collected to 1.7 A from orthorhombic crystals using synchrotron radiation and the structure was determined using a semi-automated approach. The YlxR structure resembles a two-layer alpha/beta sandwich with the overall shape of a cylinder and shows no structural homology to proteins of known structure. Structural analysis revealed that the YlxR structure represents a new protein fold that belongs to the alpha-beta plait superfamily. The distribution of the electrostatic surface potential shows a large positively charged patch on one side of the protein, a feature often found in nucleic acid-binding proteins. Three sulfate ions bind to this positively charged surface. Analysis of potential binding sites uncovered several substantial clefts, with the largest spanning 3/4 of the protein. A similar distribution of binding sites and a large sharply bent cleft are observed in RNA-binding proteins that are unrelated in sequence and structure. It is proposed that YlxR is an RNA-binding protein.
About this Structure
1G2R is a Single protein structure of sequence from Streptococcus pneumoniae. Full crystallographic information is available from OCA.
Reference
Streptococcus pneumonia YlxR at 1.35 A shows a putative new fold., Osipiuk J, Gornicki P, Maj L, Dementieva I, Laskowski R, Joachimiak A, Acta Crystallogr D Biol Crystallogr. 2001 Nov;57(Pt 11):1747-51. Epub 2001, Oct 25. PMID:11679764 Page seeded by OCA on Fri May 2 17:03:59 2008
