1d8k
From Proteopedia
OCA (Talk | contribs)
(New page: 200px<br /> <applet load="1d8k" size="450" color="white" frame="true" align="right" spinBox="true" caption="1d8k" /> '''SOLUTION STRUCTURE OF THE CENTRAL CORE DOMA...)
Next diff →
Revision as of 14:24, 12 November 2007
|
SOLUTION STRUCTURE OF THE CENTRAL CORE DOMAIN OF TFIIE BETA
Overview
Human general transcription factor TFIIE consists of two subunits, TFIIEalpha and TFIIEbeta. Recently, TFIIEbeta has been found to bind to, the region where the promoter starts to open to be single-stranded upon, transcription initiation by RNA polymerase II. Here, the central core, domain of human TFIIEbeta (TFIIEbetac) has been identified by a limited, proteolysis. This solution structure has been determined by NMR. It, consists of three helices with a beta hairpin at the C-terminus, resembling the winged helix proteins. However, TFIIEbetac shows a novel, double-stranded DNA-binding activity where the DNA-binding surface locates, on the opposite side to the previously reported winged helix motif by, forming a positively charged furrow. A model will be proposed that TFIIE, stabilizes the preinitiation complex by binding not only to the general, transcription factors together with RNA polymerase II but also to the, promoter DNA, where double-stranded DNA starts to open to be, single-stranded upon activation of the preinitiation complex.
About this Structure
1D8K is a Single protein structure of sequence from Homo sapiens. Full crystallographic information is available from OCA.
Reference
Structure of the central core domain of TFIIEbeta with a novel double-stranded DNA-binding surface., Okuda M, Watanabe Y, Okamura H, Hanaoka F, Ohkuma Y, Nishimura Y, EMBO J. 2000 Mar 15;19(6):1346-56. PMID:10716934
Page seeded by OCA on Mon Nov 12 16:30:26 2007
