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1g3v
From Proteopedia
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'''CRYSTAL STRUCTURE OF NICKEL-D[CGTGTACACG]2''' | '''CRYSTAL STRUCTURE OF NICKEL-D[CGTGTACACG]2''' | ||
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==About this Structure== | ==About this Structure== | ||
| - | + | Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1G3V OCA]. | |
==Reference== | ==Reference== | ||
Nickel-guanine interactions in DNA: crystal structure of nickel-d[CGTGTACACG]2., Abrescia NA, Huynh-Dinh T, Subirana JA, J Biol Inorg Chem. 2002 Jan;7(1-2):195-9. Epub 2001 Aug 25. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/11862555 11862555] | Nickel-guanine interactions in DNA: crystal structure of nickel-d[CGTGTACACG]2., Abrescia NA, Huynh-Dinh T, Subirana JA, J Biol Inorg Chem. 2002 Jan;7(1-2):195-9. Epub 2001 Aug 25. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/11862555 11862555] | ||
| - | [[Category: Protein complex]] | ||
[[Category: Abrescia, N G.A.]] | [[Category: Abrescia, N G.A.]] | ||
[[Category: Subirana, J A.]] | [[Category: Subirana, J A.]] | ||
| - | [[Category: | + | [[Category: B-dna]] |
| - | [[Category: | + | [[Category: Nickel binding]] |
| - | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May 2 17:06:21 2008'' | |
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | |
Revision as of 14:06, 2 May 2008
CRYSTAL STRUCTURE OF NICKEL-D[CGTGTACACG]2
Overview
The aim of this study was to clarify whether Ni2+ ions could bind to guanine bases in a standard B-DNA duplex and eventually induce a B-->Z transition. We have determined by X-ray crystallography at 3.1 A resolution the structure of the alternating deoxynucleotide d(CGTGTACACG), which contains both internal and terminal guanines. The duplex is in the B form. It is shown that nickel ions bind selectively to the N7 atom of guanine 10, which is in an extra-helical position, and guanine 2, which is in the terminal position of the duplex. It does not bind to guanine 4, which lies within a standard B-DNA tract. This simple but unambiguous result proves that nickel ions select between different guanines via steric accessibility. Guanine-Ni2+-guanine bridges among symmetry-related duplexes have also been found. These bridges may explain why Ni2+ ions may act either as a precipitant or a renaturing agent for DNA under certain conditions. The biochemical interaction of nickel with DNA can thus be related to its capacity to specifically bind to B-DNA regions with exposed guanines. Also, from the structural point of view, we have found a terminal cytosine, which forms a C.G:C reverse-Hoogsteen triple structure with a base pair of a neighbor duplex. This type of triplet is seldom found and is here described for the first time for a DNA structure.
About this Structure
Full crystallographic information is available from OCA.
Reference
Nickel-guanine interactions in DNA: crystal structure of nickel-d[CGTGTACACG]2., Abrescia NA, Huynh-Dinh T, Subirana JA, J Biol Inorg Chem. 2002 Jan;7(1-2):195-9. Epub 2001 Aug 25. PMID:11862555 Page seeded by OCA on Fri May 2 17:06:21 2008
