7bcw
From Proteopedia
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==Structure of MsbA in Salipro with ADP vanadate== | ==Structure of MsbA in Salipro with ADP vanadate== | ||
| - | <StructureSection load='7bcw' size='340' side='right'caption='[[7bcw]]' scene=''> | + | <StructureSection load='7bcw' size='340' side='right'caption='[[7bcw]], [[Resolution|resolution]] 3.50Å' scene=''> |
== Structural highlights == | == Structural highlights == | ||
| - | <table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7BCW OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7BCW FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[7bcw]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7BCW OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7BCW FirstGlance]. <br> |
| - | </td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7bcw FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7bcw OCA], [https://pdbe.org/7bcw PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7bcw RCSB], [https://www.ebi.ac.uk/pdbsum/7bcw PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7bcw ProSAT]</span></td></tr> | + | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ADP:ADENOSINE-5-DIPHOSPHATE'>ADP</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=POV:(2S)-3-(HEXADECANOYLOXY)-2-[(9Z)-OCTADEC-9-ENOYLOXY]PROPYL+2-(TRIMETHYLAMMONIO)ETHYL+PHOSPHATE'>POV</scene>, <scene name='pdbligand=VO4:VANADATE+ION'>VO4</scene></td></tr> |
| + | <tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[https://en.wikipedia.org/wiki/ABC-type_lipid_A-core_oligosaccharide_transporter ABC-type lipid A-core oligosaccharide transporter], with EC number [https://www.brenda-enzymes.info/php/result_flat.php4?ecno=7.5.2.6 7.5.2.6] </span></td></tr> | ||
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7bcw FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7bcw OCA], [https://pdbe.org/7bcw PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7bcw RCSB], [https://www.ebi.ac.uk/pdbsum/7bcw PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7bcw ProSAT]</span></td></tr> | ||
</table> | </table> | ||
| + | == Function == | ||
| + | [[https://www.uniprot.org/uniprot/MSBA_ECOLI MSBA_ECOLI]] Involved in lipid A export and possibly also in glycerophospholipid export and for biogenesis of the outer membrane. Transmembrane domains (TMD) form a pore in the inner membrane and the ATP-binding domain (NBD) is responsible for energy generation. | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | The ATP-binding cassette transporter MsbA is a lipid flippase, translocating lipid A, glycolipids, and lipopolysaccharides from the inner to the outer leaflet of the inner membrane of Gram-negative bacteria. It has been used as a model system for time-resolved structural studies as several MsbA structures in different states and reconstitution systems (detergent/nanodiscs/peptidiscs) are available. However, due to the limited resolution of the available structures, detailed structural information on the bound nucleotides has remained elusive. Here, we have reconstituted MsbA in saposin A-lipoprotein nanoparticles (Salipro) and determined the structure of ADP-vanadate-bound MsbA by single-particle cryo-electron microscopy to 3.5 A resolution. This procedure has resulted in significantly improved resolution and enabled us to model all side chains and visualise detailed ADP-vanadate interactions in the nucleotide-binding domains. The approach may be applicable to other dynamic membrane proteins. | ||
| + | |||
| + | Cryo-EM structure of MsbA in saposin-lipid nanoparticles (Salipro) provides insights into nucleotide coordination.,Kehlenbeck DM, Traore DAK, Josts I, Sander S, Moulin M, Haertlein M, Prevost S, Forsyth VT, Tidow H FEBS J. 2021 Dec 17. doi: 10.1111/febs.16327. PMID:34921499<ref>PMID:34921499</ref> | ||
| + | |||
| + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | ||
| + | </div> | ||
| + | <div class="pdbe-citations 7bcw" style="background-color:#fffaf0;"></div> | ||
| + | == References == | ||
| + | <references/> | ||
__TOC__ | __TOC__ | ||
</StructureSection> | </StructureSection> | ||
| + | [[Category: ABC-type lipid A-core oligosaccharide transporter]] | ||
[[Category: Large Structures]] | [[Category: Large Structures]] | ||
| - | [[Category: Tidow H]] | + | [[Category: Tidow, H]] |
| - | [[Category: Traore | + | [[Category: Traore, D A.K]] |
| + | [[Category: Lipid export msba]] | ||
| + | [[Category: Membrane protein]] | ||
Revision as of 07:37, 19 January 2022
Structure of MsbA in Salipro with ADP vanadate
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