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2iwt

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Revision as of 07:55, 19 January 2022

Thioredoxin h2 (HvTrxh2) in a mixed disulfide complex with the target protein BASI

Structural highlights

2iwt is a 2 chain structure with sequence from Barley. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Ligands:
Related:	1ava
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Evolutionary Conservation

Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.

Publication Abstract from PubMed

Thioredoxin is ubiquitous and regulates various target proteins through disulfide bond reduction. We report the structure of thioredoxin (HvTrxh2 from barley) in a reaction intermediate complex with a protein substrate, barley alpha-amylase/subtilisin inhibitor (BASI). The crystal structure of this mixed disulfide shows a conserved hydrophobic motif in thioredoxin interacting with a sequence of residues from BASI through van der Waals contacts and backbone-backbone hydrogen bonds. The observed structural complementarity suggests that the recognition of features around protein disulfides plays a major role in the specificity and protein disulfide reductase activity of thioredoxin. This novel insight into the function of thioredoxin constitutes a basis for comprehensive understanding of its biological role. Moreover, comparison with structurally related proteins shows that thioredoxin shares a mechanism with glutaredoxin and glutathione transferase for correctly positioning substrate cysteine residues at the catalytic groups but possesses a unique structural element that allows recognition of protein disulfides.

Structural basis for target protein recognition by the protein disulfide reductase thioredoxin.,Maeda K, Hagglund P, Finnie C, Svensson B, Henriksen A Structure. 2006 Nov;14(11):1701-10. PMID:17098195^[1]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Maeda K, Hagglund P, Finnie C, Svensson B, Henriksen A. Structural basis for target protein recognition by the protein disulfide reductase thioredoxin. Structure. 2006 Nov;14(11):1701-10. PMID:17098195 doi:10.1016/j.str.2006.09.012

1 Structural highlights
2 Evolutionary Conservation
3 Publication Abstract from PubMed
4 See Also
5 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/2iwt"

@@ Line 3: / Line 3: @@
 <StructureSection load='2iwt' size='340' side='right'caption='[[2iwt]], [[Resolution|resolution]] 2.30&Aring;' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[2iwt]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Barley Barley]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2IWT OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=2IWT FirstGlance]. <br>
+<table><tr><td colspan='2'>[[2iwt]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Barley Barley]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2IWT OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2IWT FirstGlance]. <br>
 </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=FLC:CITRATE+ANION'>FLC</scene></td></tr>
-<tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[1ava|1ava]]</td></tr>
+<tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[1ava|1ava]]</div></td></tr>
-<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=2iwt FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2iwt OCA], [http://pdbe.org/2iwt PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2iwt RCSB], [http://www.ebi.ac.uk/pdbsum/2iwt PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=2iwt ProSAT]</span></td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2iwt FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2iwt OCA], [https://pdbe.org/2iwt PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2iwt RCSB], [https://www.ebi.ac.uk/pdbsum/2iwt PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2iwt ProSAT]</span></td></tr>
 </table>
 == Evolutionary Conservation ==

2iwt

From Proteopedia

Revision as of 07:55, 19 January 2022

Thioredoxin h2 (HvTrxh2) in a mixed disulfide complex with the target protein BASI

Structural highlights

Evolutionary Conservation

Publication Abstract from PubMed

See Also

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

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