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3bxw

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Revision as of 08:28, 19 January 2022

Crystal Structure of Stabilin-1 Interacting Chitinase-Like Protein, SI-CLP

Structural highlights

3bxw is a 2 chain structure with sequence from Human. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Ligands:
Gene:	CHID1 (HUMAN)
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

[CHID1_HUMAN] Saccharide- and LPS-binding protein with possible roles in pathogen sensing and endotoxin neutralization. Ligand-binding specificity relates to the length of the oligosaccharides, with preference for chitotetraose (in vitro).^[1]

Evolutionary Conservation

Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.

Publication Abstract from PubMed

Human secreted protein stabilin-1 interacting chitinase-like protein (SI-CLP) has been identified as a novel member of Glyco_18 domain-containing proteins that is involved in host defense and inflammatory reactions. Efficient secretion of SI-CLP is mediated by its interaction with the endocytic/sorting receptor stabilin-1. SI-CLP is expressed abundantly in macrophages and neutrophils and is up-regulated by Th2 cytokine IL-4 and glucocorticoid, which suggest that SI-CLP could be a marker for adverse effects of glucocorticoid therapy. To gain insight into the biological function of SI-CLP, we determined the crystal structure of SI-CLP at 2.7 A resolution by x-ray crystallography and found that it featured a typical triose-phosphate isomerase barrel fold with a putative saccharide-binding cleft. Comparison with other chitinase-like proteins showed the cleft to be atypically wide and open. The saccharide-binding capacity of SI-CLP was investigated, and its ligand-binding specificity was found to relate to the length of the oligosaccharides, with preference for chitotetraose. Further investigations reveal that SI-CLP could bind LPS in vitro and neutralize its endotoxin effect on macrophages. Our results demonstrate the saccharide-binding property of SI-CLP by structure and in vitro biochemical analyses and suggest the possible roles of SI-CLP in pathogen sensing and endotoxin neutralization.

Structure of human stabilin-1 interacting chitinase-like protein (SI-CLP) reveals a saccharide-binding cleft with lower sugar-binding selectivity.,Meng G, Zhao Y, Bai X, Liu Y, Green TJ, Luo M, Zheng X J Biol Chem. 2010 Dec 17;285(51):39898-904. Epub 2010 Aug 19. PMID:20724479^[2]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Meng G, Zhao Y, Bai X, Liu Y, Green TJ, Luo M, Zheng X. Structure of human stabilin-1 interacting chitinase-like protein (SI-CLP) reveals a saccharide-binding cleft with lower sugar-binding selectivity. J Biol Chem. 2010 Dec 17;285(51):39898-904. Epub 2010 Aug 19. PMID:20724479 doi:10.1074/jbc.M110.130781
↑ Meng G, Zhao Y, Bai X, Liu Y, Green TJ, Luo M, Zheng X. Structure of human stabilin-1 interacting chitinase-like protein (SI-CLP) reveals a saccharide-binding cleft with lower sugar-binding selectivity. J Biol Chem. 2010 Dec 17;285(51):39898-904. Epub 2010 Aug 19. PMID:20724479 doi:10.1074/jbc.M110.130781

1 Structural highlights
2 Function
3 Evolutionary Conservation
4 Publication Abstract from PubMed
5 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/3bxw"

@@ Line 1: / Line 1: @@
 ==Crystal Structure of Stabilin-1 Interacting Chitinase-Like Protein, SI-CLP==
-<StructureSection load='3bxw' size='340' side='right' caption='[[3bxw]], [[Resolution|resolution]] 2.70&Aring;' scene=''>
+<StructureSection load='3bxw' size='340' side='right'caption='[[3bxw]], [[Resolution|resolution]] 2.70&Aring;' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[3bxw]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Human Human]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3BXW OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3BXW FirstGlance]. <br>
+<table><tr><td colspan='2'>[[3bxw]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Human Human]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3BXW OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3BXW FirstGlance]. <br>
-</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
+</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
-<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">CHID1 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN])</td></tr>
+<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">CHID1 ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN])</td></tr>
-<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3bxw FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3bxw OCA], [http://pdbe.org/3bxw PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3bxw RCSB], [http://www.ebi.ac.uk/pdbsum/3bxw PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=3bxw ProSAT]</span></td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3bxw FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3bxw OCA], [https://pdbe.org/3bxw PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3bxw RCSB], [https://www.ebi.ac.uk/pdbsum/3bxw PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3bxw ProSAT]</span></td></tr>
 </table>
 == Function ==
-[[http://www.uniprot.org/uniprot/CHID1_HUMAN CHID1_HUMAN]] Saccharide- and LPS-binding protein with possible roles in pathogen sensing and endotoxin neutralization. Ligand-binding specificity relates to the length of the oligosaccharides, with preference for chitotetraose (in vitro).<ref>PMID:20724479</ref>
+[[https://www.uniprot.org/uniprot/CHID1_HUMAN CHID1_HUMAN]] Saccharide- and LPS-binding protein with possible roles in pathogen sensing and endotoxin neutralization. Ligand-binding specificity relates to the length of the oligosaccharides, with preference for chitotetraose (in vitro).<ref>PMID:20724479</ref>
 == Evolutionary Conservation ==
 [[Image:Consurf_key_small.gif|200px|right]]
@@ Line 34: / Line 34: @@
 </StructureSection>
 [[Category: Human]]
+[[Category: Large Structures]]
 [[Category: Green, T J]]
 [[Category: Meng, G]]

3bxw

From Proteopedia

Revision as of 08:28, 19 January 2022

Crystal Structure of Stabilin-1 Interacting Chitinase-Like Protein, SI-CLP

Structural highlights

Function

Evolutionary Conservation

Publication Abstract from PubMed

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

Views

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