7m0s
From Proteopedia
(Difference between revisions)
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==N-terminal domain of PmrA from Acinetobacter baumannii== | ==N-terminal domain of PmrA from Acinetobacter baumannii== | ||
| - | <StructureSection load='7m0s' size='340' side='right'caption='[[7m0s]]' scene=''> | + | <StructureSection load='7m0s' size='340' side='right'caption='[[7m0s]], [[Resolution|resolution]] 1.64Å' scene=''> |
== Structural highlights == | == Structural highlights == | ||
| - | <table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7M0S OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7M0S FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[7m0s]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7M0S OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7M0S FirstGlance]. <br> |
</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7m0s FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7m0s OCA], [https://pdbe.org/7m0s PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7m0s RCSB], [https://www.ebi.ac.uk/pdbsum/7m0s PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7m0s ProSAT]</span></td></tr> | </td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7m0s FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7m0s OCA], [https://pdbe.org/7m0s PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7m0s RCSB], [https://www.ebi.ac.uk/pdbsum/7m0s PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7m0s ProSAT]</span></td></tr> | ||
</table> | </table> | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | Acinetobacter baumannii is an insidious emerging nosocomial pathogen that has developed resistance to all available antimicrobials, including the last resort antibiotic, colistin. Colistin resistance often occurs due to mutations in the PmrAB two component regulatory system. To better understand the regulatory mechanisms contributing to colistin resistance, we have biochemically characterized the A. baumannii PmrA response regulator. Initial DNA-binding analysis shows that A. baumannii PmrA bound to the Klebsiella pneumoniae PmrA box motif. This prompted analysis of the putative A. baumannii PmrAB regulon which indicated that the A. baumannii PmrA consensus box is 5'- HTTAAD N5 HTTAAD. Additionally, we provide the first structural information for the A. baumannii PmrA N-terminal domain through X-ray crystallography, and we present a full-length model using molecular modeling. From these studies, we were able to infer the effects of two critical PmrA mutations, PmrA::I13M and PmrA::P102R, both of which confer increased colistin resistance. Based on these data, we suggest structural and dynamic reasons for how these mutations can affect PmrA function and hence encourage resistive traits. Understanding these mechanisms will aid in the development of new targeted antimicrobial therapies. | ||
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| + | Structure of the Acinetobacter baumannii PmrA receiver domain and insights into clinical mutants affecting DNA-binding and promoting colistin resistance.,Palethorpe S, Milton ME, Pesci EC, Cavanagh J J Biochem. 2021 Sep 28. pii: 6377494. doi: 10.1093/jb/mvab102. PMID:34585233<ref>PMID:34585233</ref> | ||
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| + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | ||
| + | </div> | ||
| + | <div class="pdbe-citations 7m0s" style="background-color:#fffaf0;"></div> | ||
| + | == References == | ||
| + | <references/> | ||
__TOC__ | __TOC__ | ||
</StructureSection> | </StructureSection> | ||
[[Category: Large Structures]] | [[Category: Large Structures]] | ||
| - | [[Category: Cavanagh J]] | + | [[Category: Cavanagh, J]] |
| - | [[Category: Milton | + | [[Category: Milton, M E]] |
| - | [[Category: Palethorpe S]] | + | [[Category: Palethorpe, S]] |
| + | [[Category: Colistin resistance]] | ||
| + | [[Category: Transcription]] | ||
Revision as of 07:09, 27 January 2022
N-terminal domain of PmrA from Acinetobacter baumannii
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