This old version of Proteopedia is provided for student assignments while the new version is undergoing repairs. Content and edits done in this old version of Proteopedia after March 1, 2026 will eventually be lost when it is retired in about June of 2026.

Apply for new accounts at the new Proteopedia. Your logins will work in both the old and new versions.

2kdy

From Proteopedia

(Difference between revisions)

Jump to: navigation, search

Revision as of 07:33, 27 January 2022

NMR structure of LP2086-B01

Structural highlights

2kdy is a 1 chain structure with sequence from "diplokokkus_intracellularis_meningitidis"_(sic)_weichselbaum_1887 "diplokokkus intracellularis meningitidis" (sic) weichselbaum 1887. Full experimental information is available from OCA. For a guided tour on the structure components use FirstGlance.
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Evolutionary Conservation

Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.

Publication Abstract from PubMed

LP2086 is a family of outer membrane lipoproteins from Neisseria meningitidis, which elicits bactericidal antibodies and are currently undergoing human clinical trials in a bivalent formulation where each antigen represents one of the two known LP2086 subfamilies. Here we report the NMR structure of the recombinant LP2086 variant B01, a representative of the LP2086 subfamily B. The structure reveals a novel fold composed of two domains: a "taco-shaped" N-terminal beta-sheet and a C-terminal beta-barrel connected by a linker. The structure in micellar solution is consistent with a model of LP2086 anchored to the outer membrane bilayer through its lipidated N terminus. A long flexible chain connects the folded part of the protein to the lipid anchor and acts as spacer, making both domains accessible to the host immune system. Antibodies broadly reactive against members from both subfamilies have been mapped to the N terminus. A surface of subfamily-defining residues was identified on one face of the protein, offering an explanation for the induction of subfamily-specific bactericidal antibodies.

Structural Basis for the Immunogenic Properties of the Meningococcal Vaccine Candidate LP2086.,Mascioni A, Bentley BE, Camarda R, Dilts DA, Fink P, Gusarova V, Hoiseth SK, Jacob J, Lin SL, Malakian K, McNeil LK, Mininni T, Moy F, Murphy E, Novikova E, Sigethy S, Wen Y, Zlotnick GW, Tsao DH J Biol Chem. 2009 Mar 27;284(13):8738-46. Epub 2008 Dec 22. PMID:19103601^[1]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Mascioni A, Bentley BE, Camarda R, Dilts DA, Fink P, Gusarova V, Hoiseth SK, Jacob J, Lin SL, Malakian K, McNeil LK, Mininni T, Moy F, Murphy E, Novikova E, Sigethy S, Wen Y, Zlotnick GW, Tsao DH. Structural Basis for the Immunogenic Properties of the Meningococcal Vaccine Candidate LP2086. J Biol Chem. 2009 Mar 27;284(13):8738-46. Epub 2008 Dec 22. PMID:19103601 doi:10.1074/jbc.M808831200

1 Structural highlights
2 Evolutionary Conservation
3 Publication Abstract from PubMed
4 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/2kdy"

@@ Line 3: / Line 3: @@
 <StructureSection load='2kdy' size='340' side='right'caption='[[2kdy]], [[NMR_Ensembles_of_Models | 17 NMR models]]' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[2kdy]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/"diplokokkus_intracellularis_meningitidis"_(sic)_weichselbaum_1887 "diplokokkus intracellularis meningitidis" (sic) weichselbaum 1887]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2KDY OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=2KDY FirstGlance]. <br>
+<table><tr><td colspan='2'>[[2kdy]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/"diplokokkus_intracellularis_meningitidis"_(sic)_weichselbaum_1887 "diplokokkus intracellularis meningitidis" (sic) weichselbaum 1887]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2KDY OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2KDY FirstGlance]. <br>
-</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=2kdy FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2kdy OCA], [http://pdbe.org/2kdy PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2kdy RCSB], [http://www.ebi.ac.uk/pdbsum/2kdy PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=2kdy ProSAT]</span></td></tr>
+</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2kdy FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2kdy OCA], [https://pdbe.org/2kdy PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2kdy RCSB], [https://www.ebi.ac.uk/pdbsum/2kdy PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2kdy ProSAT]</span></td></tr>
 </table>
 == Evolutionary Conservation ==

2kdy

From Proteopedia

Revision as of 07:33, 27 January 2022

NMR structure of LP2086-B01

Structural highlights

Evolutionary Conservation

Publication Abstract from PubMed

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

Views

Personal tools

Navigation

Search

Toolbox