1g8c
From Proteopedia
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| - | + | {{STRUCTURE_1g8c| PDB=1g8c | SCENE= }} | |
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'''STRUCTURE OF THE BOVINE ANTIMICROBIAL PEPTIDE INDOLICIDIN BOUND TO SODIUM DODECYL SULFATE MICELLES''' | '''STRUCTURE OF THE BOVINE ANTIMICROBIAL PEPTIDE INDOLICIDIN BOUND TO SODIUM DODECYL SULFATE MICELLES''' | ||
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==About this Structure== | ==About this Structure== | ||
| - | 1G8C is a [[Single protein]] structure | + | 1G8C is a [[Single protein]] structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1G8C OCA]. |
==Reference== | ==Reference== | ||
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[[Category: Hancock, R E.]] | [[Category: Hancock, R E.]] | ||
[[Category: Rozek, A.]] | [[Category: Rozek, A.]] | ||
| - | [[Category: | + | [[Category: Poly-l-proline ii helix]] |
| - | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May 2 17:16:15 2008'' | |
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Revision as of 14:16, 2 May 2008
STRUCTURE OF THE BOVINE ANTIMICROBIAL PEPTIDE INDOLICIDIN BOUND TO SODIUM DODECYL SULFATE MICELLES
Overview
Indolicidin is a cationic, 13-residue antimicrobial peptide (ILPWKWPWWPWRR-NH(2)) which is unusually rich in tryptophan and proline. Its antimicrobial action involves the bacterial cytoplasmic membrane. Fluorescence and circular dichroism spectra demonstrated the structural similarity of indolicidin in complexes with large unilamellar phospolipid vesicles and with detergent micelles. The structure of indolicidin bound to zwitterionic dodecylphosphocholine (DPC) and anionic sodium dodecyl sulfate (SDS) micelles was determined using NMR methods and shown to represent a unique membrane-associated peptide structure. The backbone structure in DPC, well defined between residues 3 and 11, was extended, with two half-turns at residues Lys-5 and Trp-8. The backbone structure in SDS, well defined between residues 5 and 11, was also extended, but lacked the bend in the C-terminal half. Indolicidin in complexes with DPC had a central hydrophobic core composed of proline and tryptophan, which was bracketed by positively charged regions near the peptide termini. The tryptophan side chains, with one exception, folded flat against the peptide backbone, thus giving the molecule a wedge shape. Indolicidin in complexes with SDS had an arrangement of hydrophobic and cationic regions similar to that found in the presence of DPC. The tryptophan side chains were less well defined than for indolicidin in DPC and extended away from the peptide backbone. The preferred location of indolicidin in DPC micelles and lipid bilayers, analyzed using spin-label probes, was at the membrane interface.
About this Structure
1G8C is a Single protein structure. Full crystallographic information is available from OCA.
Reference
Structure of the bovine antimicrobial peptide indolicidin bound to dodecylphosphocholine and sodium dodecyl sulfate micelles., Rozek A, Friedrich CL, Hancock RE, Biochemistry. 2000 Dec 26;39(51):15765-74. PMID:11123901 Page seeded by OCA on Fri May 2 17:16:15 2008
