1g8t

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[[Image:1g8t.jpg|left|200px]]
[[Image:1g8t.jpg|left|200px]]
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{{Structure
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<!--
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|PDB= 1g8t |SIZE=350|CAPTION= <scene name='initialview01'>1g8t</scene>, resolution 1.1&Aring;
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The line below this paragraph, containing "STRUCTURE_1g8t", creates the "Structure Box" on the page.
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|SITE=
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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|LIGAND= <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene>
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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|ACTIVITY= <span class='plainlinks'>[http://en.wikipedia.org/wiki/Serratia_marcescens_nuclease Serratia marcescens nuclease], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.30.2 3.1.30.2] </span>
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or leave the SCENE parameter empty for the default display.
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|GENE=
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|DOMAIN=
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{{STRUCTURE_1g8t| PDB=1g8t | SCENE= }}
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|RELATEDENTRY=[[1smn|1SMN]]
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|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1g8t FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1g8t OCA], [http://www.ebi.ac.uk/pdbsum/1g8t PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1g8t RCSB]</span>
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}}
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'''SM ENDONUCLEASE FROM SERATIA MARCENSCENS AT 1.1 A RESOLUTION'''
'''SM ENDONUCLEASE FROM SERATIA MARCENSCENS AT 1.1 A RESOLUTION'''
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[[Category: Mikhailov, A M.]]
[[Category: Mikhailov, A M.]]
[[Category: Perbandt, M.]]
[[Category: Perbandt, M.]]
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[[Category: endonuclease]]
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[[Category: Endonuclease]]
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[[Category: hydrolase]]
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[[Category: Hydrolase]]
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[[Category: magnesium]]
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[[Category: Magnesium]]
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[[Category: nuclease]]
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[[Category: Nuclease]]
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[[Category: signal]]
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[[Category: Signal]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May 2 17:17:19 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 30 20:39:07 2008''
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Revision as of 14:17, 2 May 2008

Image:1g8t.jpg

Template:STRUCTURE 1g8t

SM ENDONUCLEASE FROM SERATIA MARCENSCENS AT 1.1 A RESOLUTION


Overview

The three-dimensional crystal structure of Serratia marcescens endonuclease has been refined at 1.1 A resolution to an R factor of 12.9% and an R(free) of 15.6% with the use of anisotropic temperature factors. The model contains 3694 non-H atoms, 715 water molecules, four sulfate ions and two Mg(2+)-binding sites at the active sites of the homodimeric protein. It is shown that the magnesium ion linked to the active-site Asn119 of each monomer is surrounded by five water molecules and shows an octahedral coordination geometry. The temperature factors for the bound Mg(2+) ions in the A and B subunits are 7.08 and 4.60 A(2), respectively, and the average temperature factors for the surrounding water molecules are 12.13 and 10.3 A(2), respectively. In comparison with earlier structures, alternative side-chain conformations are defined for 51 residues of the dimer, including the essential active-site residue Arg57. A plausible mechanism of enzyme function is proposed based on the high-resolution S. marcescens nuclease structure, the functional characteristics of the natural and mutational forms of the enzyme and consideration of its structural analogy with homing endo-nuclease I-PpoI.

About this Structure

1G8T is a Single protein structure of sequence from Serratia marcescens. Full crystallographic information is available from OCA.

Reference

Atomic structure of the Serratia marcescens endonuclease at 1.1 A resolution and the enzyme reaction mechanism., Shlyapnikov SV, Lunin VV, Perbandt M, Polyakov KM, Lunin VY, Levdikov VM, Betzel C, Mikhailov AM, Acta Crystallogr D Biol Crystallogr. 2000 May;56(Pt 5):567-72. PMID:10771425 Page seeded by OCA on Fri May 2 17:17:19 2008

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