7e8z
From Proteopedia
(Difference between revisions)
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==Crystal structure of the human fat mass and obesity associated protein (FTO) in complex with SS81== | ==Crystal structure of the human fat mass and obesity associated protein (FTO) in complex with SS81== | ||
| - | <StructureSection load='7e8z' size='340' side='right'caption='[[7e8z]]' scene=''> | + | <StructureSection load='7e8z' size='340' side='right'caption='[[7e8z]], [[Resolution|resolution]] 2.55Å' scene=''> |
== Structural highlights == | == Structural highlights == | ||
| - | <table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7E8Z OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7E8Z FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[7e8z]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7E8Z OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7E8Z FirstGlance]. <br> |
| - | </td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7e8z FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7e8z OCA], [https://pdbe.org/7e8z PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7e8z RCSB], [https://www.ebi.ac.uk/pdbsum/7e8z PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7e8z ProSAT]</span></td></tr> | + | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=HZX:2-[[6-[(4-nitrophenyl)amino]-3-oxidanyl-pyridin-2-yl]carbonylamino]ethanoic+acid'>HZX</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr> |
| + | <tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[4qho|4qho]]</div></td></tr> | ||
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7e8z FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7e8z OCA], [https://pdbe.org/7e8z PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7e8z RCSB], [https://www.ebi.ac.uk/pdbsum/7e8z PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7e8z ProSAT]</span></td></tr> | ||
</table> | </table> | ||
| + | == Disease == | ||
| + | [[https://www.uniprot.org/uniprot/FTO_HUMAN FTO_HUMAN]] Defects in FTO are the cause of growth retardation developmental delay coarse facies and early death (GDFD) [MIM:[https://omim.org/entry/612938 612938]]. A severe polymalformation syndrome characterized by postnatal growth retardation, microcephaly, severe psychomotor delay, functional brain deficits and characteristic facial dysmorphism. In some patients, structural brain malformations, cardiac defects, genital anomalies, and cleft palate are observed. Early death occurs by the age of 3 years.<ref>PMID:19559399</ref> | ||
| + | == Function == | ||
| + | [[https://www.uniprot.org/uniprot/FTO_HUMAN FTO_HUMAN]] Dioxygenase that repairs alkylated DNA and RNA by oxidative demethylation. Has highest activity towards single-stranded RNA containing 3-methyluracil, followed by single-stranded DNA containing 3-methylthymine. Has low demethylase activity towards single-stranded DNA containing 1-methyladenine or 3-methylcytosine. Has no activity towards 1-methylguanine. Has no detectable activity towards double-stranded DNA. Requires molecular oxygen, alpha-ketoglutarate and iron. Contributes to the regulation of the global metabolic rate, energy expenditure and energy homeostasis. Contributes to the regulation of body size and body fat accumulation.<ref>PMID:18775698</ref> <ref>PMID:20376003</ref> | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | FTO catalyzes the Fe(II) and 2-oxoglutarate (2OG)-dependent modification of nucleic acids, including the demethylation of N(6)-methyladenosine (m(6)A) in mRNA. FTO is a proposed target for anti-cancer therapy. Using information from crystal structures of FTO in complex with 2OG and substrate mimics, we designed and synthesized two series of FTO inhibitors, which were characterized by turnover and binding assays, and by X-ray crystallography with FTO and the related bacterial enzyme AlkB. A potent inhibitor employing binding interactions spanning the FTO 2OG and substrate binding sites was identified. Selectivity over other clinically targeted 2OG oxygenases was demonstrated, including with respect to the hypoxia-inducible factor prolyl and asparaginyl hydroxylases (PHD2 and FIH) and selected JmjC histone demethylases (KDMs). The results illustrate how structure-based design can enable the identification of potent and selective 2OG oxygenase inhibitors and will be useful for the development of FTO inhibitors for use in vivo. | ||
| + | |||
| + | Structure-Based Design of Selective Fat Mass and Obesity Associated Protein (FTO) Inhibitors.,Shishodia S, Demetriades M, Zhang D, Tam NY, Maheswaran P, Clunie-O'Connor C, Tumber A, Leung IKH, Ng YM, Leissing TM, El-Sagheer AH, Salah E, Brown T, Aik WS, McDonough MA, Schofield CJ J Med Chem. 2021 Nov 11. doi: 10.1021/acs.jmedchem.1c01204. PMID:34762429<ref>PMID:34762429</ref> | ||
| + | |||
| + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | ||
| + | </div> | ||
| + | <div class="pdbe-citations 7e8z" style="background-color:#fffaf0;"></div> | ||
| + | == References == | ||
| + | <references/> | ||
__TOC__ | __TOC__ | ||
</StructureSection> | </StructureSection> | ||
[[Category: Large Structures]] | [[Category: Large Structures]] | ||
| - | [[Category: Aik | + | [[Category: Aik, W S]] |
| - | [[Category: McDonough | + | [[Category: McDonough, M A]] |
| - | [[Category: Ng | + | [[Category: Ng, Y M]] |
| - | [[Category: Schofield | + | [[Category: Schofield, C J]] |
| - | [[Category: Shishodia S]] | + | [[Category: Shishodia, S]] |
| - | [[Category: Tam | + | [[Category: Tam, N Y]] |
| + | [[Category: Double-stranded beta helix]] | ||
| + | [[Category: Oxidoreductase]] | ||
| + | [[Category: Rna demethylase]] | ||
Revision as of 07:10, 2 February 2022
Crystal structure of the human fat mass and obesity associated protein (FTO) in complex with SS81
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