7p8d
From Proteopedia
(Difference between revisions)
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==Crystal structure of the Receiver domain of A. thaliana cytokinin receptor AtCRE1 in complex with Mg2+== | ==Crystal structure of the Receiver domain of A. thaliana cytokinin receptor AtCRE1 in complex with Mg2+== | ||
| - | <StructureSection load='7p8d' size='340' side='right'caption='[[7p8d]]' scene=''> | + | <StructureSection load='7p8d' size='340' side='right'caption='[[7p8d]], [[Resolution|resolution]] 1.70Å' scene=''> |
== Structural highlights == | == Structural highlights == | ||
| - | <table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7P8D OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7P8D FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[7p8d]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7P8D OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7P8D FirstGlance]. <br> |
| - | </td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7p8d FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7p8d OCA], [https://pdbe.org/7p8d PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7p8d RCSB], [https://www.ebi.ac.uk/pdbsum/7p8d PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7p8d ProSAT]</span></td></tr> | + | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=MPD:(4S)-2-METHYL-2,4-PENTANEDIOL'>MPD</scene></td></tr> |
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7p8d FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7p8d OCA], [https://pdbe.org/7p8d PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7p8d RCSB], [https://www.ebi.ac.uk/pdbsum/7p8d PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7p8d ProSAT]</span></td></tr> | ||
</table> | </table> | ||
| + | == Function == | ||
| + | [[https://www.uniprot.org/uniprot/AHK4_ARATH AHK4_ARATH]] Cytokinins (CK) receptor related to bacterial two-component regulators. Functions as a histidine kinase and transmits the stress signal to a downstream MAPK cascade. This protein undergoes an ATP-dependent autophosphorylation at a conserved histidine residue in the kinase core, and a phosphoryl group is then transferred to a conserved aspartate residue in the receiver domain. In the presence of cytokinin, feed phosphate to phosphorelay-integrating histidine phosphotransfer protein (HPt) and activates subsequent cascade. In the absence of cytokinin, removes phosphate from HPt proteins, decreasing the system phosphoload. Involved in meristems establishment in seedlings. Acts as a redundant negative regulator of drought and salt stress responses, and abscisic acid (ABA) signaling in a cytokinin-dependent manner. Required to set vascular asymmetric cell divisions that establish phloem and procambium cell lines. Redundant positive regulator of cytokinin signaling that regulates many development process including seed germination, cell division, seed size, chlorophyll retention during leaf senescence, root repression and shoot promotion. Can interact with isoprenoid-type cytokinins trans-zeatin (tZ and tZR), isopentenyladenine (iP), and isopentenyladenosine (iPR), the meta hydroxylated derivative of benzyladenine m-topolin, buta-2,3-dienyladenine (HA-8), penta-2,3-dienyladenine (HA-1), 4-methyl-penta-2,3-dienyladenine (HA-10), 4-hydroxy-2-butynyladenine (RM1), 2-butynyladenine (RM6), and to a lower extent, with cis-zeatin (cZ), zeatin riboside and dihydrozeatin (DZ). Together with AHK3, involved in the cytokinin-dependent responses to Pi starvation and sucrose stresses. Required for the formation of auxin-transporting vascular tissues in the hypocotyl, and primary and lateral roots, but not in adventitious roots, thus leading to auxin basipetal transport that regulates root development and branching. Involved in alkamides (e.g. N-isobutyl decanamide) and N-acylethanolamides (NAE) signaling that control meristematic activity and differentiation processes during plant development. Prevents the uptake of sulfate by mediating cytokinin-dependent down-regulation of high-affinity sulfate transporters (e.g. SULTR1;1 and SULTR1;2) expression in roots. Together with AHK2, required for growth and reproduction promotion stimulated by the endophytic fungus Piriformospora indica in a trans-zeatin-dependent manner. Required to trigger the phytotoxic effect of the snapdragon (Antirrhinum majus) flowers volatile organic compound (VOC) methyl benzoate (MB). Plays a role in the cytokinin-mediated repression of the iron uptake pathway.<ref>PMID:11114883</ref> <ref>PMID:11234017</ref> <ref>PMID:11230563</ref> <ref>PMID:11479382</ref> <ref>PMID:11577198</ref> <ref>PMID:12354925</ref> <ref>PMID:12410813</ref> <ref>PMID:15155880</ref> <ref>PMID:15509853</ref> <ref>PMID:15053761</ref> <ref>PMID:15144379</ref> <ref>PMID:15166290</ref> <ref>PMID:16246292</ref> <ref>PMID:15923327</ref> <ref>PMID:15728338</ref> <ref>PMID:16753566</ref> <ref>PMID:17075078</ref> <ref>PMID:16361392</ref> <ref>PMID:16357038</ref> <ref>PMID:17216481</ref> <ref>PMID:17956858</ref> <ref>PMID:18077346</ref> <ref>PMID:17965178</ref> <ref>PMID:18571199</ref> <ref>PMID:18785832</ref> <ref>PMID:18397377</ref> <ref>PMID:19032596</ref> <ref>PMID:20110319</ref> | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | Cytokinins are phytohormones regulating many biological processes that are vital to plants. CYTOKININ RESPONSE1 (CRE1), the main cytokinin receptor, has a modular architecture composed of a cytokinin-binding CHASE (Cyclases/Histidine kinases Associated Sensory Extracellular) domain, followed by a transmembrane fragment, an intracellular histidine kinase (HK) domain, and a receiver domain (REC). Perception of cytokinin signaling involves (i) a hormone molecule binding to the CHASE domain, (ii) CRE1 autophosphorylation at a conserved His residue in the HK domain, followed by a phosphorelay to (iii) a conserved Asp residue in the REC domain, (iv) a histidine-containing phosphotransfer protein (HPt), and (v) a response regulator (RR). This work focuses on the crystal structures of the REC domain of CRE1 from the model plant Arabidopsis thaliana and from the model legume Medicago truncatula. Both REC domains form tight 3D-domain-swapped dimers. Dimerization of the REC domain agrees with the quaternary assembly of the entire CRE1 but is incompatible with a model of its complex with HPt, suggesting that a considerable conformational change should occur to enable the signal transduction. Indeed, phosphorylation of the REC domain can change the HPt-binding properties of CRE1, as shown by functional studies. | ||
| + | |||
| + | 3D Domain Swapping Dimerization of the Receiver Domain of Cytokinin Receptor CRE1 From Arabidopsis thaliana and Medicago truncatula.,Tran LH, Urbanowicz A, Jasinski M, Jaskolski M, Ruszkowski M Front Plant Sci. 2021 Sep 24;12:756341. doi: 10.3389/fpls.2021.756341., eCollection 2021. PMID:34630499<ref>PMID:34630499</ref> | ||
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| + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | ||
| + | </div> | ||
| + | <div class="pdbe-citations 7p8d" style="background-color:#fffaf0;"></div> | ||
| + | == References == | ||
| + | <references/> | ||
__TOC__ | __TOC__ | ||
</StructureSection> | </StructureSection> | ||
[[Category: Large Structures]] | [[Category: Large Structures]] | ||
| - | [[Category: Jasinski M]] | + | [[Category: Jasinski, M]] |
| - | [[Category: Jaskolski M]] | + | [[Category: Jaskolski, M]] |
| - | [[Category: Ruszkowski M]] | + | [[Category: Ruszkowski, M]] |
| - | [[Category: Tran | + | [[Category: Tran, L H]] |
| - | [[Category: Urbanowicz A]] | + | [[Category: Urbanowicz, A]] |
| + | [[Category: Cytokinin]] | ||
| + | [[Category: Flavodoxin-like fold]] | ||
| + | [[Category: Phosphorelay]] | ||
| + | [[Category: Signaling protein]] | ||
Revision as of 07:14, 2 February 2022
Crystal structure of the Receiver domain of A. thaliana cytokinin receptor AtCRE1 in complex with Mg2+
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