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7qfp
From Proteopedia
(Difference between revisions)
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==Cryo-EM structure of Botulinum neurotoxin serotype E== | ==Cryo-EM structure of Botulinum neurotoxin serotype E== | ||
| - | <StructureSection load='7qfp' size='340' side='right'caption='[[7qfp]]' scene=''> | + | <StructureSection load='7qfp' size='340' side='right'caption='[[7qfp]], [[Resolution|resolution]] 3.70Å' scene=''> |
== Structural highlights == | == Structural highlights == | ||
| - | <table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7QFP OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7QFP FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[7qfp]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7QFP OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7QFP FirstGlance]. <br> |
</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7qfp FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7qfp OCA], [https://pdbe.org/7qfp PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7qfp RCSB], [https://www.ebi.ac.uk/pdbsum/7qfp PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7qfp ProSAT]</span></td></tr> | </td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7qfp FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7qfp OCA], [https://pdbe.org/7qfp PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7qfp RCSB], [https://www.ebi.ac.uk/pdbsum/7qfp PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7qfp ProSAT]</span></td></tr> | ||
</table> | </table> | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | Botulinum neurotoxins (BoNTs) are the causative agents of a potentially lethal paralytic disease targeting cholinergic nerve terminals. Multiple BoNT serotypes exist, with types A, B and E being the main cause of human botulism. Their extreme toxicity has been exploited for cosmetic and therapeutic uses to treat a wide range of neuromuscular disorders. Although naturally occurring BoNT types share a common end effect, their activity varies significantly based on the neuronal cell-surface receptors and intracellular SNARE substrates they target. These properties are the result of structural variations that have traditionally been studied using biophysical methods such as X-ray crystallography. Here, we determined the first structures of botulinum neurotoxins using single-particle cryogenic electron microscopy. The maps obtained at 3.6 and 3.7 A for BoNT/B and /E, respectively, highlight the subtle structural dynamism between domains, and of the binding domain in particular. This study demonstrates how the recent advances made in the field of single-particle electron microscopy can be applied to bacterial toxins of clinical relevance and the botulinum neurotoxin family in particular. | ||
| + | |||
| + | Structural Analysis of Botulinum Neurotoxins Type B and E by Cryo-EM.,Kosenina S, Martinez-Carranza M, Davies JR, Masuyer G, Stenmark P Toxins (Basel). 2021 Dec 23;14(1). pii: toxins14010014. doi:, 10.3390/toxins14010014. PMID:35050991<ref>PMID:35050991</ref> | ||
| + | |||
| + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | ||
| + | </div> | ||
| + | <div class="pdbe-citations 7qfp" style="background-color:#fffaf0;"></div> | ||
| + | == References == | ||
| + | <references/> | ||
__TOC__ | __TOC__ | ||
</StructureSection> | </StructureSection> | ||
[[Category: Large Structures]] | [[Category: Large Structures]] | ||
| - | [[Category: Davies | + | [[Category: Davies, J R]] |
| - | [[Category: Kosenina S]] | + | [[Category: Kosenina, S]] |
| - | [[Category: Martinez-Carranza M]] | + | [[Category: Martinez-Carranza, M]] |
| - | [[Category: Masuyer G]] | + | [[Category: Masuyer, G]] |
| - | [[Category: Stenmark P]] | + | [[Category: Stenmark, P]] |
| + | [[Category: Clostridium botulinum]] | ||
| + | [[Category: Neurotoxin]] | ||
| + | [[Category: Toxin]] | ||
Current revision
Cryo-EM structure of Botulinum neurotoxin serotype E
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