2l37

From Proteopedia

(Difference between revisions)

Revision as of 07:38, 2 February 2022

3D solution structure of arginine/glutamate-rich polypeptide Luffin P1 from the seeds of sponge gourd (Luffa cylindrical)

Structural highlights

2l37 is a 1 chain structure with sequence from Lufae. Full experimental information is available from OCA. For a guided tour on the structure components use FirstGlance.
Activity:	rRNA N-glycosylase, with EC number 3.2.2.22
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

[RIP1_LUFAE] Inhibits protein synthesis in animal cells.^[1]

Publication Abstract from PubMed

Luffin P1, the smallest ribosome-inactivating peptide from the seeds of Luffa cylindrica was found to have anti-HIV-1 activity in HIV-1 infected C8166 T-cell lines and be able to bind with HIV Rev Response Element. Nuclear magnetic resonance spectroscopy revealed that the Luffin P1 comprises a helix-loop-helix motif, with the two alpha helices tightly associated by two disulfide bonds. Based on our findings, we conclude that unlike the well-studied ribosome-inactivating proteins, which exert their action through N-glycosidase activities, Luffin P1 demonstrates a novel inactivation mechanism probably through the charge complementation with viral or cellular proteins. Our work also provides a new scaffold for the design of novel inhibitors from a simple helical motif.

Structural characterization and anti-HIV-1 activities of arginine/glutamate-rich polypeptide Luffin P1 from the seeds of sponge gourd (Luffa cylindrica).,Ng YM, Yang Y, Sze KH, Zhang X, Zheng YT, Shaw PC J Struct Biol. 2010 Dec 31. PMID:21195767^[2]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Li F, Yang XX, Xia HC, Zeng R, Hu WG, Li Z, Zhang ZC. Purification and characterization of Luffin P1, a ribosome-inactivating peptide from the seeds of Luffa cylindrica. Peptides. 2003 Jun;24(6):799-805. PMID:12948831
↑ Ng YM, Yang Y, Sze KH, Zhang X, Zheng YT, Shaw PC. Structural characterization and anti-HIV-1 activities of arginine/glutamate-rich polypeptide Luffin P1 from the seeds of sponge gourd (Luffa cylindrica). J Struct Biol. 2010 Dec 31. PMID:21195767 doi:10.1016/j.jsb.2010.12.007

1 Structural highlights
2 Function
3 Publication Abstract from PubMed
4 See Also
5 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/2l37"

@@ Line 3: / Line 3: @@
 <StructureSection load='2l37' size='340' side='right'caption='[[2l37]], [[NMR_Ensembles_of_Models | 20 NMR models]]' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[2l37]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Lufae Lufae]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2L37 OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=2L37 FirstGlance]. <br>
+<table><tr><td colspan='2'>[[2l37]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Lufae Lufae]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2L37 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2L37 FirstGlance]. <br>
-</td></tr><tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/rRNA_N-glycosylase rRNA N-glycosylase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.2.22 3.2.2.22] </span></td></tr>
+</td></tr><tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[https://en.wikipedia.org/wiki/rRNA_N-glycosylase rRNA N-glycosylase], with EC number [https://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.2.22 3.2.2.22] </span></td></tr>
-<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=2l37 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2l37 OCA], [http://pdbe.org/2l37 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2l37 RCSB], [http://www.ebi.ac.uk/pdbsum/2l37 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=2l37 ProSAT]</span></td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2l37 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2l37 OCA], [https://pdbe.org/2l37 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2l37 RCSB], [https://www.ebi.ac.uk/pdbsum/2l37 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2l37 ProSAT]</span></td></tr>
 </table>
 == Function ==
-[[http://www.uniprot.org/uniprot/RIP1_LUFAE RIP1_LUFAE]] Inhibits protein synthesis in animal cells.<ref>PMID:12948831</ref>
+[[https://www.uniprot.org/uniprot/RIP1_LUFAE RIP1_LUFAE]] Inhibits protein synthesis in animal cells.<ref>PMID:12948831</ref>
 <div style="background-color:#fffaf0;">
 == Publication Abstract from PubMed ==
@@ Line 18: / Line 18: @@
 </div>
 <div class="pdbe-citations 2l37" style="background-color:#fffaf0;"></div>
+==See Also==
+*[[Ribosome inactivating protein|Ribosome inactivating protein]]
 == References ==
 <references/>

2l37

From Proteopedia

Revision as of 07:38, 2 February 2022

3D solution structure of arginine/glutamate-rich polypeptide Luffin P1 from the seeds of sponge gourd (Luffa cylindrical)

Structural highlights

Function

Publication Abstract from PubMed

See Also

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

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