1dm4

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spinqualitylabelsall models 1dm4, resolution 2.50Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image

SER195ALA MUTANT OF HUMAN THROMBIN COMPLEXED WITH FIBRINOPEPTIDE A (7-16)

Contents 1 Overview 2 Disease 3 About this Structure 4 Reference

Overview

The Ser195Ala mutant of human alpha-thrombin was complexed with, fibrinopeptide A(7-22) (FPA) in an effort to describe the (P1'-P6'), post-cleavage binding subsites of the fibrinogen-recognition exosite and, define more clearly the nature of the Michaelis complex and the scissile, peptide bond bound at the catalytic site. The thrombin mutant, however, has residual catalytic activity and proteolysis occurred at the, Arg16-Gly17 bond. Thus, the structure of the thrombin complex determined, was that of FPA(7-16) bound at the active site, which is very similar to, the ternary FPA(7-16)cmk-human thrombin-hirugen complex (r.m.s.d., approximately 0.4 A; Stubbs et al. , 1992). It is further shown by, subsidiary experiments that the cleavage is the result of residual, catalytic activity of the altered catalytic machinery.

Disease

Known diseases associated with this structure: Dysprothrombinemia OMIM:[176930], Hyperprothrombinemia OMIM:[176930], Hypoprothrombinemia OMIM:[176930]

About this Structure

1DM4 is a Protein complex structure of sequences from Homo sapiens with ACE as ligand. Full crystallographic information is available from OCA.

Reference

Structure of the Ser195Ala mutant of human alpha--thrombin complexed with fibrinopeptide A(7--16): evidence for residual catalytic activity., Krishnan R, Sadler JE, Tulinsky A, Acta Crystallogr D Biol Crystallogr. 2000 Apr;56(Pt 4):406-10. PMID:10739913

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