1gcy

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[[Image:1gcy.gif|left|200px]]
[[Image:1gcy.gif|left|200px]]
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{{Structure
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<!--
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|PDB= 1gcy |SIZE=350|CAPTION= <scene name='initialview01'>1gcy</scene>, resolution 1.60&Aring;
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The line below this paragraph, containing "STRUCTURE_1gcy", creates the "Structure Box" on the page.
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|SITE=
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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|LIGAND= <scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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|ACTIVITY= <span class='plainlinks'>[http://en.wikipedia.org/wiki/Glucan_1,4-alpha-maltotetraohydrolase Glucan 1,4-alpha-maltotetraohydrolase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.60 3.2.1.60] </span>
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or leave the SCENE parameter empty for the default display.
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|GENE=
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|DOMAIN=
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{{STRUCTURE_1gcy| PDB=1gcy | SCENE= }}
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|RELATEDENTRY=
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|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1gcy FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1gcy OCA], [http://www.ebi.ac.uk/pdbsum/1gcy PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1gcy RCSB]</span>
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}}
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'''HIGH RESOLUTION CRYSTAL STRUCTURE OF MALTOTETRAOSE-FORMING EXO-AMYLASE'''
'''HIGH RESOLUTION CRYSTAL STRUCTURE OF MALTOTETRAOSE-FORMING EXO-AMYLASE'''
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[[Category: Matsuura, Y.]]
[[Category: Matsuura, Y.]]
[[Category: Mezaki, Y.]]
[[Category: Mezaki, Y.]]
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[[Category: beta sheet]]
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[[Category: Beta sheet]]
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[[Category: beta-alpha-barrel]]
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[[Category: Beta-alpha-barrel]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May 2 17:25:35 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 30 20:41:34 2008''
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Revision as of 14:25, 2 May 2008

Image:1gcy.gif

Template:STRUCTURE 1gcy

HIGH RESOLUTION CRYSTAL STRUCTURE OF MALTOTETRAOSE-FORMING EXO-AMYLASE


Overview

The intact maltotetraose-forming exo-amylase from Pseudomonas stutzeri (G4-1), which has a raw starch binding domain, has been crystallized. The structure was identified (PDB entry 1GCY) by the molecular replacement method using the structure of its catalytic domain (G4-2). The result showed that the raw starch binding domain is in a disordered state, the corresponding electron densities being almost invisible. Superposition of these two enzyme forms showed evidence for the possible location of the raw starch binding domain (SBD). This crystal is a novel case, in that it forms a regular lattice incorporating flexibly bound SBD in the channel of crystal packing of the catalytic domains.

About this Structure

1GCY is a Single protein structure of sequence from Pseudomonas stutzeri. Full crystallographic information is available from OCA.

Reference

Crystallization and structural analysis of intact maltotetraose-forming exo-amylase from Pseudomonas stutzeri., Mezaki Y, Katsuya Y, Kubota M, Matsuura Y, Biosci Biotechnol Biochem. 2001 Jan;65(1):222-5. PMID:11272837 Page seeded by OCA on Fri May 2 17:25:35 2008

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