7doj
From Proteopedia
(Difference between revisions)
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==Solution structure of TGS domain of the Mycobacterium tuberculosis Rel protein== | ==Solution structure of TGS domain of the Mycobacterium tuberculosis Rel protein== | ||
| - | <StructureSection load='7doj' size='340' side='right'caption='[[7doj]]' scene=''> | + | <StructureSection load='7doj' size='340' side='right'caption='[[7doj]], [[NMR_Ensembles_of_Models | 19 NMR models]]' scene=''> |
== Structural highlights == | == Structural highlights == | ||
| - | <table><tr><td colspan='2'>Full | + | <table><tr><td colspan='2'>[[7doj]] is a 1 chain structure. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7DOJ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7DOJ FirstGlance]. <br> |
| - | </td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7doj FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7doj OCA], [https://pdbe.org/7doj PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7doj RCSB], [https://www.ebi.ac.uk/pdbsum/7doj PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7doj ProSAT]</span></td></tr> | + | </td></tr><tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[https://en.wikipedia.org/wiki/GTP_diphosphokinase GTP diphosphokinase], with EC number [https://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.6.5 2.7.6.5] </span></td></tr> |
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7doj FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7doj OCA], [https://pdbe.org/7doj PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7doj RCSB], [https://www.ebi.ac.uk/pdbsum/7doj PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7doj ProSAT]</span></td></tr> | ||
</table> | </table> | ||
| + | == Function == | ||
| + | [[https://www.uniprot.org/uniprot/A0A655DL01_MYCTX A0A655DL01_MYCTX]] In eubacteria ppGpp (guanosine 3'-diphosphate 5'-diphosphate) is a mediator of the stringent response that coordinates a variety of cellular activities in response to changes in nutritional abundance.[RuleBase:RU003847] | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | The stringent response is critical for the survival of Mycobacterium tuberculosis (Mtb) under nutrient starvation. The mechanism is mediated by a GTP pyrophosphokinase known as Rel, containing N-terminal synthetase and hydrolase domains and C-terminal regulatory domains, which include the TGS domain (ThrRS, GTPase, and SpoT proteins) that has been proposed to activate the synthetase domain via interaction with deacylated tRNA. Here, we present the NMR solution structure of the Mtb Rel TGS domain (MtRel TGS), consisting of five antiparallel beta-strands and one helix-loop-helix motif. The interaction of MtRel TGS with deacylated tRNA is shown, indicating the critical amino acids of MtRel TGS in tRNA binding, and presenting the first structural evidence of MtRel TGS binding to deacylated tRNA in solution in the absence of the translational machinery. | ||
| + | |||
| + | Atomic structure of the regulatory TGS domain of Rel protein from Mycobacterium tuberculosis and its interaction with deacylated tRNA.,Shin J, Singal B, Gruber A, Wong DMK, Ragunathan P, Gruber G FEBS Lett. 2021 Nov 22. doi: 10.1002/1873-3468.14236. PMID:34808002<ref>PMID:34808002</ref> | ||
| + | |||
| + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | ||
| + | </div> | ||
| + | <div class="pdbe-citations 7doj" style="background-color:#fffaf0;"></div> | ||
| + | == References == | ||
| + | <references/> | ||
__TOC__ | __TOC__ | ||
</StructureSection> | </StructureSection> | ||
| + | [[Category: GTP diphosphokinase]] | ||
[[Category: Large Structures]] | [[Category: Large Structures]] | ||
| - | [[Category: Grueber G]] | + | [[Category: Grueber, G]] |
| - | [[Category: Joon S]] | + | [[Category: Joon, S]] |
| - | [[Category: Singal B]] | + | [[Category: Singal, B]] |
| + | [[Category: Mycobacterium tuberculosis]] | ||
| + | [[Category: Rel]] | ||
| + | [[Category: Rna binding protein]] | ||
| + | [[Category: Stringent response]] | ||
| + | [[Category: Tgs domain]] | ||
| + | [[Category: Transferase]] | ||
| + | [[Category: Trna binding]] | ||
Revision as of 10:51, 16 February 2022
Solution structure of TGS domain of the Mycobacterium tuberculosis Rel protein
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