7e2s
From Proteopedia
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==Synechocystis GUN4 in complex with biliverdin IXa== | ==Synechocystis GUN4 in complex with biliverdin IXa== | ||
| - | <StructureSection load='7e2s' size='340' side='right'caption='[[7e2s]]' scene=''> | + | <StructureSection load='7e2s' size='340' side='right'caption='[[7e2s]], [[Resolution|resolution]] 1.05Å' scene=''> |
== Structural highlights == | == Structural highlights == | ||
| - | <table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7E2S OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7E2S FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[7e2s]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Syny3 Syny3]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7E2S OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7E2S FirstGlance]. <br> |
| - | </td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7e2s FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7e2s OCA], [https://pdbe.org/7e2s PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7e2s RCSB], [https://www.ebi.ac.uk/pdbsum/7e2s PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7e2s ProSAT]</span></td></tr> | + | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=BLA:BILIVERDINE+IX+ALPHA'>BLA</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene></td></tr> |
| + | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">sll0558 ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1111708 SYNY3])</td></tr> | ||
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7e2s FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7e2s OCA], [https://pdbe.org/7e2s PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7e2s RCSB], [https://www.ebi.ac.uk/pdbsum/7e2s PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7e2s ProSAT]</span></td></tr> | ||
</table> | </table> | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | The chlorophyll biosynthesis regulator GENOMES UNCOUPLED 4 (GUN4) is conserved in nearly all oxygenic photosynthetic organisms. Recently, GUN4 has been found to be able to bind the linear tetrapyrroles (bilins) and stimulate the magnesium chelatase activity in the unicellular green alga Chlamydomonas reinhardtii. Here, we characterize GUN4 proteins from Arabidopsis thaliana and the cyanobacterium Synechocystis sp. PCC 6803 for their ability to bind bilins, and present the crystal structures of Synechocystis GUN4 in biliverdin-bound, phycocyanobilin-bound, and phytochromobilin-bound forms at the resolutions of 1.05, 1.10, and 1.70 A, respectively. These linear molecules adopt a cyclic-helical conformation, and bind more tightly than planar porphyrins to the tetrapyrrole-binding pocket of GUN4. Based on structural comparison, we propose a working model of GUN4 in regulation of tetrapyrrole biosynthetic pathway, and address the role of the bilin-bound GUN4 in retrograde signaling. | ||
| + | |||
| + | Structural basis of bilin binding by the chlorophyll biosynthesis regulator GUN4.,Hu JH, Chang JW, Xu T, Wang J, Wang X, Lin R, Duanmu D, Liu L Protein Sci. 2021 Aug 11. doi: 10.1002/pro.4164. PMID:34382282<ref>PMID:34382282</ref> | ||
| + | |||
| + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | ||
| + | </div> | ||
| + | <div class="pdbe-citations 7e2s" style="background-color:#fffaf0;"></div> | ||
| + | == References == | ||
| + | <references/> | ||
__TOC__ | __TOC__ | ||
</StructureSection> | </StructureSection> | ||
[[Category: Large Structures]] | [[Category: Large Structures]] | ||
| - | [[Category: Hu J]] | + | [[Category: Syny3]] |
| - | [[Category: Liu L]] | + | [[Category: Hu, J]] |
| + | [[Category: Liu, L]] | ||
| + | [[Category: Gun4]] | ||
| + | [[Category: Ligand binding protein]] | ||
| + | [[Category: Signaling protein]] | ||
Revision as of 10:52, 16 February 2022
Synechocystis GUN4 in complex with biliverdin IXa
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