7ehf
From Proteopedia
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==Crystal structure of the aminoglycoside resistance methyltransferase NpmB1== | ==Crystal structure of the aminoglycoside resistance methyltransferase NpmB1== | ||
| - | <StructureSection load='7ehf' size='340' side='right'caption='[[7ehf]]' scene=''> | + | <StructureSection load='7ehf' size='340' side='right'caption='[[7ehf]], [[Resolution|resolution]] 1.50Å' scene=''> |
== Structural highlights == | == Structural highlights == | ||
| - | <table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7EHF OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7EHF FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[7ehf]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/"bacillus_coli"_migula_1895 "bacillus coli" migula 1895]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7EHF OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7EHF FirstGlance]. <br> |
| - | </td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7ehf FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7ehf OCA], [https://pdbe.org/7ehf PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7ehf RCSB], [https://www.ebi.ac.uk/pdbsum/7ehf PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7ehf ProSAT]</span></td></tr> | + | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene></td></tr> |
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7ehf FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7ehf OCA], [https://pdbe.org/7ehf PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7ehf RCSB], [https://www.ebi.ac.uk/pdbsum/7ehf PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7ehf ProSAT]</span></td></tr> | ||
</table> | </table> | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | Post-translational methylation of the A site of 16S rRNA at position A1408 leads to pan-aminoglycoside resistance encompassing both 4,5- and 4,6-disubstituted 2-deoxystreptamine (DOS) aminoglycosides. To date, NpmA is the only acquired enzyme with such function. Here, we present function and structure of NpmB1 whose sequence was identified in Escherichia coli genomes registered from the United Kingdom. NpmB1 possesses 40% amino acid identity with NpmA1 and confers resistance to all clinically relevant aminoglycosides including 4,5-DOS agents. Phylogenetic analysis of NpmB1 and NpmB2, its single amino acid variant, revealed that the encoding gene was likely acquired by E. coli from a soil bacterium. The structure of NpmB1 suggests that it requires a structural change of the beta6/7 linker in order to bind to 16S rRNA. These findings establish NpmB1 and NpmB2 as the second group of acquired pan-aminoglycoside resistance 16S rRNA methyltransferases. | ||
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| + | Functional and Structural Characterization of Acquired Pan-Aminoglycoside Resistance 16S rRNA Methyltransferase NpmB1.,Kawai A, Suzuki M, Tsukamoto K, Minato Y, Doi Y Antimicrob Agents Chemother. 2021 Jul 26:AAC0100921. doi: 10.1128/AAC.01009-21. PMID:34310216<ref>PMID:34310216</ref> | ||
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| + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | ||
| + | </div> | ||
| + | <div class="pdbe-citations 7ehf" style="background-color:#fffaf0;"></div> | ||
| + | == References == | ||
| + | <references/> | ||
__TOC__ | __TOC__ | ||
</StructureSection> | </StructureSection> | ||
| + | [[Category: Bacillus coli migula 1895]] | ||
[[Category: Large Structures]] | [[Category: Large Structures]] | ||
| - | [[Category: Doi Y]] | + | [[Category: Doi, Y]] |
| - | [[Category: Kawai A]] | + | [[Category: Kawai, A]] |
| + | [[Category: 16s rrna methyltransferase]] | ||
| + | [[Category: Transferase]] | ||
Revision as of 10:54, 16 February 2022
Crystal structure of the aminoglycoside resistance methyltransferase NpmB1
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