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7vbs
From Proteopedia
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==Structure of the AAA+ ATPase domain of the transcriptional regulator GtrR in Burkholderia cenocepacia== | ==Structure of the AAA+ ATPase domain of the transcriptional regulator GtrR in Burkholderia cenocepacia== | ||
| - | <StructureSection load='7vbs' size='340' side='right'caption='[[7vbs]]' scene=''> | + | <StructureSection load='7vbs' size='340' side='right'caption='[[7vbs]], [[Resolution|resolution]] 2.00Å' scene=''> |
== Structural highlights == | == Structural highlights == | ||
| - | <table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7VBS OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7VBS FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[7vbs]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7VBS OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7VBS FirstGlance]. <br> |
| - | </td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7vbs FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7vbs OCA], [https://pdbe.org/7vbs PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7vbs RCSB], [https://www.ebi.ac.uk/pdbsum/7vbs PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7vbs ProSAT]</span></td></tr> | + | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene></td></tr> |
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7vbs FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7vbs OCA], [https://pdbe.org/7vbs PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7vbs RCSB], [https://www.ebi.ac.uk/pdbsum/7vbs PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7vbs ProSAT]</span></td></tr> | ||
</table> | </table> | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | Global transcriptional regulator downstream RpfR (GtrR) is a key downstream regulator for quorum-sensing signaling molecule cis-2-dodecenoic acid (BDSF). As a bacterial enhancer-binding protein (bEBP), GtrR is composed of an N-terminal receiver domain, a central ATPases associated with diverse cellular activities (AAA+) ATPase sigma(54) -interaction domain, and a C-terminal helix-turn-helix DNA-binding domain. In this work, we solved its AAA+ ATPase domain in both apo and GTP-bound forms. The structure revealed how GtrR specifically recognizes GTP. In addition, we also revealed that GtrR has moderate GTPase activity in vitro in the absence of its activation signal. Finally, we found the residues K170, D236, R311, and R357 in GtrR that are crucial to its biological function, any single mutation leading to completely abolishing GtrR activity. | ||
| + | |||
| + | Structural analyses of the AAA+ ATPase domain of the transcriptional regulator GtrR in the BDSF quorum-sensing system in Burkholderia cenocepacia.,Yan XF, Yang C, Wang M, Yong Y, Deng Y, Gao YG FEBS Lett. 2021 Nov 27. doi: 10.1002/1873-3468.14244. PMID:34837384<ref>PMID:34837384</ref> | ||
| + | |||
| + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | ||
| + | </div> | ||
| + | <div class="pdbe-citations 7vbs" style="background-color:#fffaf0;"></div> | ||
| + | == References == | ||
| + | <references/> | ||
__TOC__ | __TOC__ | ||
</StructureSection> | </StructureSection> | ||
[[Category: Large Structures]] | [[Category: Large Structures]] | ||
| - | [[Category: Gao | + | [[Category: Gao, Y G]] |
| - | [[Category: Yan | + | [[Category: Yan, X F]] |
| - | [[Category: Yong Y]] | + | [[Category: Yong, Y]] |
| + | [[Category: Aaa+ atpase]] | ||
| + | [[Category: Bacterial enhancer-binding protein]] | ||
| + | [[Category: Quorum sensing]] | ||
| + | [[Category: Transcription]] | ||
| + | [[Category: Transcriptional regulator]] | ||
Revision as of 11:12, 16 February 2022
Structure of the AAA+ ATPase domain of the transcriptional regulator GtrR in Burkholderia cenocepacia
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