7vf0
From Proteopedia
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==Crystal structure of Cyclosorus parasiticus chalcone synthase 1 (CpCHS1) complex with naringenin and CoA== | ==Crystal structure of Cyclosorus parasiticus chalcone synthase 1 (CpCHS1) complex with naringenin and CoA== | ||
| - | <StructureSection load='7vf0' size='340' side='right'caption='[[7vf0]]' scene=''> | + | <StructureSection load='7vf0' size='340' side='right'caption='[[7vf0]], [[Resolution|resolution]] 2.40Å' scene=''> |
== Structural highlights == | == Structural highlights == | ||
| - | <table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7VF0 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7VF0 FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[7vf0]] is a 4 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7VF0 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7VF0 FirstGlance]. <br> |
| - | </td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7vf0 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7vf0 OCA], [https://pdbe.org/7vf0 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7vf0 RCSB], [https://www.ebi.ac.uk/pdbsum/7vf0 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7vf0 ProSAT]</span></td></tr> | + | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=COA:COENZYME+A'>COA</scene>, <scene name='pdbligand=NAR:NARINGENIN'>NAR</scene></td></tr> |
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7vf0 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7vf0 OCA], [https://pdbe.org/7vf0 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7vf0 RCSB], [https://www.ebi.ac.uk/pdbsum/7vf0 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7vf0 ProSAT]</span></td></tr> | ||
</table> | </table> | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | The biosynthesis of flavonoids and anthocyanidins has been exclusively investigated in angiosperms but largely unknown in ferns. This study integrated metabolomics and transcriptome to analyze the fronds from different development stages (S1 without spores and S2 with brown spores) of Cyclosorus parasiticus. About 221 flavonoid and anthocyanin metabolites were identified between S1 and S2. Transcriptome analysis revealed several genes encoding the key enzymes involved in the biosynthesis of flavonoids, and anthocyanins were upregulated in S2, which were validated by qRT-PCR. Functional characterization of two chalcone synthases (CpCHS1 and CpCHS2) indicated that CpCHS1 can catalyze the formation of pinocembrin, naringenin, and eriodictyol, respectively; however, CpCHS2 was inactive. The crystallization investigation of CpCHS1 indicated that it has a highly similar conformation and shares a similar general catalytic mechanism to other plants CHSs. And by site-directed mutagenesis, we found seven residues, especially Leu199 and Thr203 that are critical to the catalytic activity for CpCHS1. | ||
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| + | Functional and Structural Investigation of Chalcone Synthases Based on Integrated Metabolomics and Transcriptome Analysis on Flavonoids and Anthocyanins Biosynthesis of the Fern Cyclosorus parasiticus.,Niu M, Fu J, Ni R, Xiong RL, Zhu TT, Lou HX, Zhang P, Li J, Cheng AX Front Plant Sci. 2021 Oct 28;12:757516. doi: 10.3389/fpls.2021.757516., eCollection 2021. PMID:34777436<ref>PMID:34777436</ref> | ||
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| + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | ||
| + | </div> | ||
| + | <div class="pdbe-citations 7vf0" style="background-color:#fffaf0;"></div> | ||
| + | == References == | ||
| + | <references/> | ||
__TOC__ | __TOC__ | ||
</StructureSection> | </StructureSection> | ||
[[Category: Large Structures]] | [[Category: Large Structures]] | ||
| - | [[Category: Cheng | + | [[Category: Cheng, A X]] |
| - | [[Category: Li | + | [[Category: Li, J X]] |
| + | [[Category: Chalcone synthase]] | ||
| + | [[Category: Coa]] | ||
| + | [[Category: Cyclosorus parasiticus]] | ||
| + | [[Category: Flavonoids biosynthesis]] | ||
| + | [[Category: Naringenin]] | ||
| + | [[Category: Transferase]] | ||
Revision as of 11:13, 16 February 2022
Crystal structure of Cyclosorus parasiticus chalcone synthase 1 (CpCHS1) complex with naringenin and CoA
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