3ee1

From Proteopedia

(Difference between revisions)

Revision as of 11:47, 16 February 2022

Novel fold of VirA, a type III secretion system effector protein from Shigella flexneri

Structural highlights

3ee1 is a 2 chain structure with sequence from "shigella_paradysenteriae"_weldin_1927 "shigella paradysenteriae" weldin 1927. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
NonStd Res:
Gene:	virA ("Shigella paradysenteriae" Weldin 1927)
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

[VIRA_SHIFL] Alpha-tubulin-specific protease that is required for entry into epithelial cells and for subsequent intra- and intercellular spreading. Contributes to bacterial entry into epithelial cells by inducing microtubule (MT) destabilization and the formation of membrane ruffles. The membrane ruffling evoked by VirA results from the activation of host rac1, which is associated with the destruction of MT networks. Creates a tunnel inside the host cell cytoplasm by breaking down the microtubule infrastructure. This facilitates the bacterium's movement through the cytoplasm and also helps other bacteria move faster during the invasion of the eukaryotic cell. Is absolutely required for virulence.^[1] ^[2] ^[3]

Publication Abstract from PubMed

VirA, a secreted effector protein from Shigella sp., has been shown to be necessary for its virulence. It was also reported that VirA might be related to papain-like cysteine proteases and cleave alpha-tubulin, thus facilitating intracellular spreading. We have now determined the crystal structure of VirA at 3.0 A resolution. The shape of the molecule resembles the letter "V," with the residues in the N-terminal third of the 45-kDa molecule (some of which are disordered) forming one clearly identifiable domain, and the remainder of the molecule completing the V-like structure. The fold of VirA is unique and does not resemble that of any known protein, including papain, although its N-terminal domain is topologically similar to cysteine protease inhibitors such as stefin B. Analysis of the sequence conservation between VirA and its Escherichia coli homologs EspG and EspG2 did not result in identification of any putative protease-like active site, leaving open a possibility that the biological function of VirA in Shigella virulence may not involve direct proteolytic activity.

Novel fold of VirA, a type III secretion system effector protein from Shigella flexneri.,Davis J, Wang J, Tropea JE, Zhang D, Dauter Z, Waugh DS, Wlodawer A Protein Sci. 2008 Dec;17(12):2167-73. Epub 2008 Sep 11. PMID:18787201^[4]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Uchiya K, Tobe T, Komatsu K, Suzuki T, Watarai M, Fukuda I, Yoshikawa M, Sasakawa C. Identification of a novel virulence gene, virA, on the large plasmid of Shigella, involved in invasion and intercellular spreading. Mol Microbiol. 1995 Jul;17(2):241-50. PMID:7494473
↑ Yoshida S, Katayama E, Kuwae A, Mimuro H, Suzuki T, Sasakawa C. Shigella deliver an effector protein to trigger host microtubule destabilization, which promotes Rac1 activity and efficient bacterial internalization. EMBO J. 2002 Jun 17;21(12):2923-35. PMID:12065406 doi:10.1093/emboj/cdf319
↑ Yoshida S, Handa Y, Suzuki T, Ogawa M, Suzuki M, Tamai A, Abe A, Katayama E, Sasakawa C. Microtubule-severing activity of Shigella is pivotal for intercellular spreading. Science. 2006 Nov 10;314(5801):985-9. PMID:17095701 doi:10.1126/science.1133174
↑ Davis J, Wang J, Tropea JE, Zhang D, Dauter Z, Waugh DS, Wlodawer A. Novel fold of VirA, a type III secretion system effector protein from Shigella flexneri. Protein Sci. 2008 Dec;17(12):2167-73. Epub 2008 Sep 11. PMID:18787201 doi:ps.037978.108

1 Structural highlights
2 Function
3 Publication Abstract from PubMed
4 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/3ee1"

@@ Line 1: / Line 1: @@
 ==Novel fold of VirA, a type III secretion system effector protein from Shigella flexneri==
-<StructureSection load='3ee1' size='340' side='right' caption='[[3ee1]], [[Resolution|resolution]] 3.01&Aring;' scene=''>
+<StructureSection load='3ee1' size='340' side='right'caption='[[3ee1]], [[Resolution|resolution]] 3.01&Aring;' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[3ee1]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/"shigella_paradysenteriae"_weldin_1927 "shigella paradysenteriae" weldin 1927]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3EE1 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3EE1 FirstGlance]. <br>
+<table><tr><td colspan='2'>[[3ee1]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/"shigella_paradysenteriae"_weldin_1927 "shigella paradysenteriae" weldin 1927]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3EE1 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3EE1 FirstGlance]. <br>
 </td></tr><tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr>
-<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">virA ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=623 "Shigella paradysenteriae" Weldin 1927])</td></tr>
+<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">virA ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=623 "Shigella paradysenteriae" Weldin 1927])</td></tr>
-<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3ee1 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3ee1 OCA], [http://pdbe.org/3ee1 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3ee1 RCSB], [http://www.ebi.ac.uk/pdbsum/3ee1 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=3ee1 ProSAT]</span></td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3ee1 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3ee1 OCA], [https://pdbe.org/3ee1 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3ee1 RCSB], [https://www.ebi.ac.uk/pdbsum/3ee1 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3ee1 ProSAT]</span></td></tr>
 </table>
 == Function ==
-[[http://www.uniprot.org/uniprot/VIRA_SHIFL VIRA_SHIFL]] Alpha-tubulin-specific protease that is required for entry into epithelial cells and for subsequent intra- and intercellular spreading. Contributes to bacterial entry into epithelial cells by inducing microtubule (MT) destabilization and the formation of membrane ruffles. The membrane ruffling evoked by VirA results from the activation of host rac1, which is associated with the destruction of MT networks. Creates a tunnel inside the host cell cytoplasm by breaking down the microtubule infrastructure. This facilitates the bacterium's movement through the cytoplasm and also helps other bacteria move faster during the invasion of the eukaryotic cell. Is absolutely required for virulence.<ref>PMID:7494473</ref> <ref>PMID:12065406</ref> <ref>PMID:17095701</ref>
+[[https://www.uniprot.org/uniprot/VIRA_SHIFL VIRA_SHIFL]] Alpha-tubulin-specific protease that is required for entry into epithelial cells and for subsequent intra- and intercellular spreading. Contributes to bacterial entry into epithelial cells by inducing microtubule (MT) destabilization and the formation of membrane ruffles. The membrane ruffling evoked by VirA results from the activation of host rac1, which is associated with the destruction of MT networks. Creates a tunnel inside the host cell cytoplasm by breaking down the microtubule infrastructure. This facilitates the bacterium's movement through the cytoplasm and also helps other bacteria move faster during the invasion of the eukaryotic cell. Is absolutely required for virulence.<ref>PMID:7494473</ref> <ref>PMID:12065406</ref> <ref>PMID:17095701</ref>
 <div style="background-color:#fffaf0;">
 == Publication Abstract from PubMed ==
@@ Line 24: / Line 24: @@
 </StructureSection>
 [[Category: Shigella paradysenteriae weldin 1927]]
+[[Category: Large Structures]]
 [[Category: Davis, J S]]
 [[Category: Beta barrel]]

3ee1

From Proteopedia

Revision as of 11:47, 16 February 2022

Novel fold of VirA, a type III secretion system effector protein from Shigella flexneri

Structural highlights

Function

Publication Abstract from PubMed

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

Views

Personal tools

Navigation

Search

Toolbox