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| | ==Crystal structure of the human COPII-coat protein Sec24c== | | ==Crystal structure of the human COPII-coat protein Sec24c== |
| - | <StructureSection load='3eh2' size='340' side='right' caption='[[3eh2]], [[Resolution|resolution]] 2.35Å' scene=''> | + | <StructureSection load='3eh2' size='340' side='right'caption='[[3eh2]], [[Resolution|resolution]] 2.35Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[3eh2]] is a 3 chain structure with sequence from [http://en.wikipedia.org/wiki/Human Human]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3EH2 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3EH2 FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[3eh2]] is a 3 chain structure with sequence from [https://en.wikipedia.org/wiki/Human Human]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3EH2 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3EH2 FirstGlance]. <br> |
| - | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr> | + | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr> |
| - | <tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[3efo|3efo]], [[3eg9|3eg9]], [[3egd|3egd]], [[3eh1|3eh1]], [[3egx|3egx]]</td></tr> | + | <tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[3efo|3efo]], [[3eg9|3eg9]], [[3egd|3egd]], [[3eh1|3eh1]], [[3egx|3egx]]</div></td></tr> |
| - | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">SEC24C ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN])</td></tr> | + | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">SEC24C ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN])</td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3eh2 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3eh2 OCA], [http://pdbe.org/3eh2 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3eh2 RCSB], [http://www.ebi.ac.uk/pdbsum/3eh2 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=3eh2 ProSAT]</span></td></tr> | + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3eh2 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3eh2 OCA], [https://pdbe.org/3eh2 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3eh2 RCSB], [https://www.ebi.ac.uk/pdbsum/3eh2 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3eh2 ProSAT]</span></td></tr> |
| | </table> | | </table> |
| | == Function == | | == Function == |
| - | [[http://www.uniprot.org/uniprot/SC24C_HUMAN SC24C_HUMAN]] Component of the COPII coat, that covers ER-derived vesicles involved in transport from the endoplasmic reticulum to the Golgi apparatus. COPII acts in the cytoplasm to promote the transport of secretory, plasma membrane, and vacuolar proteins from the endoplasmic reticulum to the Golgi complex.<ref>PMID:10214955</ref> <ref>PMID:10329445</ref> <ref>PMID:10075675</ref> | + | [[https://www.uniprot.org/uniprot/SC24C_HUMAN SC24C_HUMAN]] Component of the COPII coat, that covers ER-derived vesicles involved in transport from the endoplasmic reticulum to the Golgi apparatus. COPII acts in the cytoplasm to promote the transport of secretory, plasma membrane, and vacuolar proteins from the endoplasmic reticulum to the Golgi complex.<ref>PMID:10214955</ref> <ref>PMID:10329445</ref> <ref>PMID:10075675</ref> |
| | == Evolutionary Conservation == | | == Evolutionary Conservation == |
| | [[Image:Consurf_key_small.gif|200px|right]] | | [[Image:Consurf_key_small.gif|200px|right]] |
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| | </StructureSection> | | </StructureSection> |
| | [[Category: Human]] | | [[Category: Human]] |
| | + | [[Category: Large Structures]] |
| | [[Category: Goldberg, J]] | | [[Category: Goldberg, J]] |
| | [[Category: Mancias, J D]] | | [[Category: Mancias, J D]] |
| Structural highlights
Function
[SC24C_HUMAN] Component of the COPII coat, that covers ER-derived vesicles involved in transport from the endoplasmic reticulum to the Golgi apparatus. COPII acts in the cytoplasm to promote the transport of secretory, plasma membrane, and vacuolar proteins from the endoplasmic reticulum to the Golgi complex.[1] [2] [3]
Evolutionary Conservation
Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.
Publication Abstract from PubMed
Genomic analysis shows that the increased complexity of trafficking pathways in mammalian cells involves an expansion of the number of SNARE, Rab and COP proteins. Thus, the human genome encodes four forms of Sec24, the cargo selection subunit of the COPII vesicular coat, and this is proposed to increase the range of cargo accommodated by human COPII-coated vesicles. In this study, we combined X-ray crystallographic and biochemical analysis with functional assays of cargo packaging into COPII vesicles to establish molecular mechanisms for cargo discrimination by human Sec24 subunits. A conserved IxM packaging signal binds in a surface groove of Sec24c and Sec24d, but the groove is occluded in the Sec24a and Sec24b subunits. Conversely, LxxLE class transport signals and the DxE signal of VSV glycoprotein are selectively bound by Sec24a and Sec24b subunits. A comparative analysis of crystal structures of the four human Sec24 isoforms establishes the structural determinants for discrimination among these transport signals, and provides a framework to understand how an expansion of coat subunits extends the range of cargo proteins packaged into COPII-coated vesicles.
Structural basis of cargo membrane protein discrimination by the human COPII coat machinery.,Mancias JD, Goldberg J EMBO J. 2008 Nov 5;27(21):2918-28. Epub 2008 Oct 9. PMID:18843296[4]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ Tani K, Oyama Y, Hatsuzawa K, Tagaya M. Hypothetical protein KIAA0079 is a mammalian homologue of yeast Sec24p. FEBS Lett. 1999 Mar 26;447(2-3):247-50. PMID:10214955
- ↑ Tang BL, Kausalya J, Low DY, Lock ML, Hong W. A family of mammalian proteins homologous to yeast Sec24p. Biochem Biophys Res Commun. 1999 May 19;258(3):679-84. PMID:10329445 doi:http://dx.doi.org/10.1006/bbrc.1999.0574
- ↑ Pagano A, Letourneur F, Garcia-Estefania D, Carpentier JL, Orci L, Paccaud JP. Sec24 proteins and sorting at the endoplasmic reticulum. J Biol Chem. 1999 Mar 19;274(12):7833-40. PMID:10075675
- ↑ Mancias JD, Goldberg J. Structural basis of cargo membrane protein discrimination by the human COPII coat machinery. EMBO J. 2008 Nov 5;27(21):2918-28. Epub 2008 Oct 9. PMID:18843296 doi:http://dx.doi.org/10.1038/emboj.2008.208
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