7eyp
From Proteopedia
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==Crystal structure of Pseudomonas aeruginosa ppnP== | ==Crystal structure of Pseudomonas aeruginosa ppnP== | ||
| - | <StructureSection load='7eyp' size='340' side='right'caption='[[7eyp]]' scene=''> | + | <StructureSection load='7eyp' size='340' side='right'caption='[[7eyp]], [[Resolution|resolution]] 1.50Å' scene=''> |
== Structural highlights == | == Structural highlights == | ||
| - | <table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7EYP OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7EYP FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[7eyp]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7EYP OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7EYP FirstGlance]. <br> |
</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7eyp FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7eyp OCA], [https://pdbe.org/7eyp PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7eyp RCSB], [https://www.ebi.ac.uk/pdbsum/7eyp PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7eyp ProSAT]</span></td></tr> | </td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7eyp FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7eyp OCA], [https://pdbe.org/7eyp PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7eyp RCSB], [https://www.ebi.ac.uk/pdbsum/7eyp PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7eyp ProSAT]</span></td></tr> | ||
</table> | </table> | ||
| + | == Function == | ||
| + | [[https://www.uniprot.org/uniprot/A0A072ZTW6_PSEAI A0A072ZTW6_PSEAI]] Catalyzes the phosphorolysis of diverse nucleosides, yielding D-ribose 1-phosphate and the respective free bases. Can use uridine, adenosine, guanosine, cytidine, thymidine, inosine and xanthosine as substrates. Also catalyzes the reverse reactions.[HAMAP-Rule:MF_01537] | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | Nucleotides metabolism is a fundamental process in all organisms. Two families of nucleoside phosphorylases (NP) that catalyze the phosphorolytic cleavage of the glycosidic bond in nucleosides have been found, including the trimeric or hexameric NP-I and dimeric NP-II family enzymes. Recent studies revealed another class of NP protein in Escherichia coli named Pyrimidine/purine nucleoside phosphorylase (ppnP), which can catalyze the phosphorolysis of diverse nucleosides and yield d-ribose 1-phosphate and the respective free bases. Here, we solved the crystal structures of ppnP from E. coli and the other three species. Our studies revealed that the structure of ppnP belongs to the RlmC-like Cupin fold and showed as a rigid dimeric conformation. Detail analysis revealed a potential nucleoside binding pocket full of hydrophobic residues, and the residues involved in the dimer and pocket formation are all well conserved in bacteria. Since the Cupin fold is a large superfamily in the biosynthesis of natural products, our studies provide the structural basis for understanding, and the directed evolution of NP proteins. | ||
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| + | Crystal structures of a new class of pyrimidine/purine nucleoside phosphorylase revealed a Cupin fold.,Wen Y, Li X, Guo W, Wu B Proteins. 2022 Jan 30. doi: 10.1002/prot.26309. PMID:35094440<ref>PMID:35094440</ref> | ||
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| + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | ||
| + | </div> | ||
| + | <div class="pdbe-citations 7eyp" style="background-color:#fffaf0;"></div> | ||
| + | == References == | ||
| + | <references/> | ||
__TOC__ | __TOC__ | ||
</StructureSection> | </StructureSection> | ||
[[Category: Large Structures]] | [[Category: Large Structures]] | ||
| - | [[Category: Wen Y]] | + | [[Category: Wen, Y]] |
| - | [[Category: Wu | + | [[Category: Wu, B X]] |
| + | [[Category: Hydrolase]] | ||
| + | [[Category: Nucleoside phosphorylase]] | ||
| + | [[Category: Purine]] | ||
| + | [[Category: Pyrimidine]] | ||
Revision as of 08:38, 23 February 2022
Crystal structure of Pseudomonas aeruginosa ppnP
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