7lve

From Proteopedia

(Difference between revisions)

Revision as of 08:40, 23 February 2022

Jug r 2 Leader Sequence Residues 117-161

Structural highlights

7lve is a 1 chain structure. Full experimental information is available from OCA. For a guided tour on the structure components use FirstGlance.
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

[VCL2_JUGRE] Seed storage protein.^[1] ^[2] ^[3] ^[4]

Publication Abstract from PubMed

Vicilin-buried peptides (VBPs) from edible plants are derived from the N-terminal leader sequences (LSs) of seed storage proteins. VBPs are defined by a common alpha-hairpin fold mediated by conserved CxxxCx(10-14)CxxxC motifs. Here, peanut and walnut VBPs were characterized as potential mediators of both peanut/walnut allergenicity and cross-reactivity despite their low ( approximately 17%) sequence identity. The structures of one peanut (AH1.1) and 3 walnut (JR2.1, JR2.2, JR2.3) VBPs were solved using solution NMR, revealing similar alpha-hairpin structures stabilized by disulfide bonds with high levels of surface similarity. Peptide microarrays identified several peptide sequences primarily on AH1.1 and JR2.1, which were recognized by peanut-, walnut-, and dual-allergic patient IgE, establishing these peanut and walnut VBPs as potential mediators of allergenicity and cross-reactivity. JR2.2 and JR2.3 displayed extreme resilience against endosomal digestion, potentially hindering epitope generation and likely contributing to their reduced allergic potential.

Structure, Immunogenicity, and IgE Cross-Reactivity among Walnut and Peanut Vicilin-Buried Peptides.,Foo ACY, Nesbit JB, Gipson SAY, Cheng H, Bushel P, DeRose EF, Schein CH, Teuber SS, Hurlburt BK, Maleki SJ, Mueller GA J Agric Food Chem. 2022 Feb 9. doi: 10.1021/acs.jafc.1c07225. PMID:35139305^[5]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Teuber SS, Jarvis KC, Dandekar AM, Peterson WR, Ansari AA. Identification and cloning of a complementary DNA encoding a vicilin-like proprotein, jug r 2, from english walnut kernel (Juglans regia), a major food allergen. J Allergy Clin Immunol. 1999 Dec;104(6):1311-20. doi:, 10.1016/s0091-6749(99)70029-1. PMID:10589017 doi:http://dx.doi.org/10.1016/s0091-6749(99)70029-1
↑ Maleki SJ, Teuber SS, Cheng H, Chen D, Comstock SS, Ruan S, Schein CH. Computationally predicted IgE epitopes of walnut allergens contribute to cross-reactivity with peanuts. Allergy. 2011 Dec;66(12):1522-9. doi: 10.1111/j.1398-9995.2011.02692.x. Epub 2011, Aug 23. PMID:21883278 doi:http://dx.doi.org/10.1111/j.1398-9995.2011.02692.x
↑ Downs ML, Semic-Jusufagic A, Simpson A, Bartra J, Fernandez-Rivas M, Rigby NM, Taylor SL, Baumert JL, Mills EN. Characterization of low molecular weight allergens from English walnut (Juglans regia). J Agric Food Chem. 2014 Dec 3;62(48):11767-75. doi: 10.1021/jf504672m. Epub 2014 , Nov 20. PMID:25388987 doi:http://dx.doi.org/10.1021/jf504672m
↑ Dubiela P, Kabasser S, Smargiasso N, Geiselhart S, Bublin M, Hafner C, Mazzucchelli G, Hoffmann-Sommergruber K. Jug r 6 is the allergenic vicilin present in walnut responsible for IgE cross-reactivities to other tree nuts and seeds. Sci Rep. 2018 Jul 27;8(1):11366. doi: 10.1038/s41598-018-29656-4. PMID:30054513 doi:http://dx.doi.org/10.1038/s41598-018-29656-4
↑ Foo ACY, Nesbit JB, Gipson SAY, Cheng H, Bushel P, DeRose EF, Schein CH, Teuber SS, Hurlburt BK, Maleki SJ, Mueller GA. Structure, Immunogenicity, and IgE Cross-Reactivity among Walnut and Peanut Vicilin-Buried Peptides. J Agric Food Chem. 2022 Feb 9. doi: 10.1021/acs.jafc.1c07225. PMID:35139305 doi:http://dx.doi.org/10.1021/acs.jafc.1c07225

1 Structural highlights
2 Function
3 Publication Abstract from PubMed
4 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/7lve"

@@ Line 1: / Line 1: @@
 ==Jug r 2 Leader Sequence Residues 117-161==
-<StructureSection load='7lve' size='340' side='right'caption='[[7lve]]' scene=''>
+<StructureSection load='7lve' size='340' side='right'caption='[[7lve]], [[NMR_Ensembles_of_Models | 20 NMR models]]' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7LVE OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7LVE FirstGlance]. <br>
+<table><tr><td colspan='2'>[[7lve]] is a 1 chain structure. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7LVE OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7LVE FirstGlance]. <br>
 </td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7lve FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7lve OCA], [https://pdbe.org/7lve PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7lve RCSB], [https://www.ebi.ac.uk/pdbsum/7lve PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7lve ProSAT]</span></td></tr>
 </table>
+== Function ==
+[[https://www.uniprot.org/uniprot/VCL2_JUGRE VCL2_JUGRE]] Seed storage protein.<ref>PMID:10589017</ref> <ref>PMID:21883278</ref> <ref>PMID:25388987</ref> <ref>PMID:30054513</ref>
+<div style="background-color:#fffaf0;">
+== Publication Abstract from PubMed ==
+Vicilin-buried peptides (VBPs) from edible plants are derived from the N-terminal leader sequences (LSs) of seed storage proteins. VBPs are defined by a common alpha-hairpin fold mediated by conserved CxxxCx(10-14)CxxxC motifs. Here, peanut and walnut VBPs were characterized as potential mediators of both peanut/walnut allergenicity and cross-reactivity despite their low ( approximately 17%) sequence identity. The structures of one peanut (AH1.1) and 3 walnut (JR2.1, JR2.2, JR2.3) VBPs were solved using solution NMR, revealing similar alpha-hairpin structures stabilized by disulfide bonds with high levels of surface similarity. Peptide microarrays identified several peptide sequences primarily on AH1.1 and JR2.1, which were recognized by peanut-, walnut-, and dual-allergic patient IgE, establishing these peanut and walnut VBPs as potential mediators of allergenicity and cross-reactivity. JR2.2 and JR2.3 displayed extreme resilience against endosomal digestion, potentially hindering epitope generation and likely contributing to their reduced allergic potential.
+Structure, Immunogenicity, and IgE Cross-Reactivity among Walnut and Peanut Vicilin-Buried Peptides.,Foo ACY, Nesbit JB, Gipson SAY, Cheng H, Bushel P, DeRose EF, Schein CH, Teuber SS, Hurlburt BK, Maleki SJ, Mueller GA J Agric Food Chem. 2022 Feb 9. doi: 10.1021/acs.jafc.1c07225. PMID:35139305<ref>PMID:35139305</ref>
+From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
+</div>
+<div class="pdbe-citations 7lve" style="background-color:#fffaf0;"></div>
+== References ==
+<references/>
 __TOC__
 </StructureSection>
 [[Category: Large Structures]]
-[[Category: DeRose EF]]
+[[Category: DeRose, E F]]
-[[Category: Foo ACY]]
+[[Category: Foo, A C.Y]]
-[[Category: Mueller GA]]
+[[Category: Mueller, G A]]
+[[Category: Allergen]]
+[[Category: Seed storage]]

7lve

From Proteopedia

Revision as of 08:40, 23 February 2022

Jug r 2 Leader Sequence Residues 117-161

Structural highlights

Function

Publication Abstract from PubMed

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

Views

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