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| | ==Structure of the signal receiver domain of the putative response regulator NarL from Mycobacterium tuberculosis== | | ==Structure of the signal receiver domain of the putative response regulator NarL from Mycobacterium tuberculosis== |
| - | <StructureSection load='3eul' size='340' side='right' caption='[[3eul]], [[Resolution|resolution]] 1.90Å' scene=''> | + | <StructureSection load='3eul' size='340' side='right'caption='[[3eul]], [[Resolution|resolution]] 1.90Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[3eul]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/"bacillus_tuberculosis"_(zopf_1883)_klein_1884 "bacillus tuberculosis" (zopf 1883) klein 1884]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3EUL OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3EUL FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[3eul]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/"bacillus_tuberculosis"_(zopf_1883)_klein_1884 "bacillus tuberculosis" (zopf 1883) klein 1884]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3EUL OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3EUL FirstGlance]. <br> |
| - | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene></td></tr> | + | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene></td></tr> |
| - | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">MT0866, narL, Rv0844c ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1773 "Bacillus tuberculosis" (Zopf 1883) Klein 1884])</td></tr> | + | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">MT0866, narL, Rv0844c ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1773 "Bacillus tuberculosis" (Zopf 1883) Klein 1884])</td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3eul FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3eul OCA], [http://pdbe.org/3eul PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3eul RCSB], [http://www.ebi.ac.uk/pdbsum/3eul PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=3eul ProSAT]</span></td></tr> | + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3eul FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3eul OCA], [https://pdbe.org/3eul PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3eul RCSB], [https://www.ebi.ac.uk/pdbsum/3eul PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3eul ProSAT]</span></td></tr> |
| | </table> | | </table> |
| | == Function == | | == Function == |
| - | [[http://www.uniprot.org/uniprot/NARL_MYCTU NARL_MYCTU]] Probable member of the two-component regulatory system NarS/NarL.<ref>PMID:12595424</ref> | + | [[https://www.uniprot.org/uniprot/NARL_MYCTU NARL_MYCTU]] Probable member of the two-component regulatory system NarS/NarL.<ref>PMID:12595424</ref> |
| | == Evolutionary Conservation == | | == Evolutionary Conservation == |
| | [[Image:Consurf_key_small.gif|200px|right]] | | [[Image:Consurf_key_small.gif|200px|right]] |
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| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| | + | [[Category: Large Structures]] |
| | [[Category: Agren, D]] | | [[Category: Agren, D]] |
| | [[Category: Schneider, G]] | | [[Category: Schneider, G]] |
| Structural highlights
Function
[NARL_MYCTU] Probable member of the two-component regulatory system NarS/NarL.[1]
Evolutionary Conservation
Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.
Publication Abstract from PubMed
NarL from Mycobacterium tuberculosis is a putative nitrate response regulator that is involved in the regulation of anaerobic metabolism in this pathogen. The recombinant purified N-terminal signal receiver domain of NarL has been crystallized in space group C222(1), with unit-cell parameters a = 85.6, b = 90.0, c = 126.3 A, and the structure was determined by molecular replacement to 1.9 A resolution. Comparisons with related signal receiver domains show that the closest structural homologue is an uncharacterized protein from Staphylococcus aureus, whereas the nearest sequence homologue, NarL from Escherichia coli, displays larger differences in three-dimensional structure. The largest differences between the mycobacterial and E. coli NarL domains were found in the loop between beta3 and alpha3 in the proximity of the phosphorylation site. The active site in response regulators is similar to that of members of the haloacid dehalogenase (HAD) family, which also form a phospho-aspartyl intermediate. In NarL, the aspartic acid that acts as catalytic acid/base in several HAD enzymes is replaced by an arginine residue, which is less likely to participate in steps involving proton abstraction. This substitution may slow down the breakdown of the phospho-aspartyl anhydride and allow signalling beyond the timescales defined by a catalytic reaction intermediate.
1.9 A structure of the signal receiver domain of the putative response regulator NarL from Mycobacterium tuberculosis.,Schnell R, Agren D, Schneider G Acta Crystallogr Sect F Struct Biol Cryst Commun. 2008 Dec 1;64(Pt, 12):1096-100. Epub 2008 Nov 28. PMID:19052358[2]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ Parish T, Smith DA, Kendall S, Casali N, Bancroft GJ, Stoker NG. Deletion of two-component regulatory systems increases the virulence of Mycobacterium tuberculosis. Infect Immun. 2003 Mar;71(3):1134-40. PMID:12595424
- ↑ Schnell R, Agren D, Schneider G. 1.9 A structure of the signal receiver domain of the putative response regulator NarL from Mycobacterium tuberculosis. Acta Crystallogr Sect F Struct Biol Cryst Commun. 2008 Dec 1;64(Pt, 12):1096-100. Epub 2008 Nov 28. PMID:19052358 doi:10.1107/S1744309108035203
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