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| | ==Crystal structure of 3-ketoacyl-(acyl-carrier-protein) reductase Rickettsia prowazekii== | | ==Crystal structure of 3-ketoacyl-(acyl-carrier-protein) reductase Rickettsia prowazekii== |
| - | <StructureSection load='3f9i' size='340' side='right' caption='[[3f9i]], [[Resolution|resolution]] 2.25Å' scene=''> | + | <StructureSection load='3f9i' size='340' side='right'caption='[[3f9i]], [[Resolution|resolution]] 2.25Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[3f9i]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Atcc_vr-142 Atcc vr-142]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3F9I OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3F9I FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[3f9i]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Atcc_vr-142 Atcc vr-142]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3F9I OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3F9I FirstGlance]. <br> |
| - | </td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">fabG, RP762 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=782 ATCC VR-142])</td></tr> | + | </td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">fabG, RP762 ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=782 ATCC VR-142])</td></tr> |
| - | <tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/3-oxoacyl-[acyl-carrier-protein]_reductase 3-oxoacyl-[acyl-carrier-protein] reductase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.1.1.100 1.1.1.100] </span></td></tr> | + | <tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[https://en.wikipedia.org/wiki/3-oxoacyl-[acyl-carrier-protein]_reductase 3-oxoacyl-[acyl-carrier-protein] reductase], with EC number [https://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.1.1.100 1.1.1.100] </span></td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3f9i FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3f9i OCA], [http://pdbe.org/3f9i PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3f9i RCSB], [http://www.ebi.ac.uk/pdbsum/3f9i PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=3f9i ProSAT]</span></td></tr> | + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3f9i FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3f9i OCA], [https://pdbe.org/3f9i PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3f9i RCSB], [https://www.ebi.ac.uk/pdbsum/3f9i PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3f9i ProSAT]</span></td></tr> |
| | </table> | | </table> |
| | == Function == | | == Function == |
| - | [[http://www.uniprot.org/uniprot/FABG_RICPR FABG_RICPR]] Catalyzes the NADPH-dependent reduction of beta-ketoacyl-ACP substrates to beta-hydroxyacyl-ACP products, the first reductive step in the elongation cycle of fatty acid biosynthesis (By similarity). | + | [[https://www.uniprot.org/uniprot/FABG_RICPR FABG_RICPR]] Catalyzes the NADPH-dependent reduction of beta-ketoacyl-ACP substrates to beta-hydroxyacyl-ACP products, the first reductive step in the elongation cycle of fatty acid biosynthesis (By similarity). |
| | == Evolutionary Conservation == | | == Evolutionary Conservation == |
| | [[Image:Consurf_key_small.gif|200px|right]] | | [[Image:Consurf_key_small.gif|200px|right]] |
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| | ==See Also== | | ==See Also== |
| - | *[[Beta-ketoacyl-ACP reductase|Beta-ketoacyl-ACP reductase]] | + | *[[Beta-ketoacyl carrier protein reductase 3D structures|Beta-ketoacyl carrier protein reductase 3D structures]] |
| | == References == | | == References == |
| | <references/> | | <references/> |
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| | </StructureSection> | | </StructureSection> |
| | [[Category: Atcc vr-142]] | | [[Category: Atcc vr-142]] |
| | + | [[Category: Large Structures]] |
| | [[Category: Structural genomic]] | | [[Category: Structural genomic]] |
| | [[Category: Fatty acid biosynthesis]] | | [[Category: Fatty acid biosynthesis]] |
| Structural highlights
Function
[FABG_RICPR] Catalyzes the NADPH-dependent reduction of beta-ketoacyl-ACP substrates to beta-hydroxyacyl-ACP products, the first reductive step in the elongation cycle of fatty acid biosynthesis (By similarity).
Evolutionary Conservation
Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.
Publication Abstract from PubMed
Rickettsia prowazekii, a parasitic Gram-negative bacterium, is in the second-highest biodefense category of pathogens of the National Institute of Allergy and Infectious Diseases, but only a handful of structures have been deposited in the PDB for this bacterium; to date, all of these have been solved by the SSGCID. Owing to its small genome (about 800 protein-coding genes), it relies on the host for many basic biosynthetic processes, hindering the identification of potential antipathogenic drug targets. However, like many bacteria and plants, its metabolism does depend upon the type II fatty-acid synthesis (FAS) pathway for lipogenesis, whereas the predominant form of fatty-acid biosynthesis in humans is via the type I pathway. Here, the structure of the third enzyme in the FAS pathway, 3-ketoacyl-(acyl-carrier-protein) reductase, is reported at a resolution of 2.25 A. Its fold is highly similar to those of the existing structures from some well characterized pathogens, such as Mycobacterium tuberculosis and Burkholderia pseudomallei, but differs significantly from the analogous mammalian structure. Hence, drugs known to target the enzymes of pathogenic bacteria may serve as potential leads against Rickettsia, which is responsible for spotted fever and typhus and is found throughout the world.
Structure of 3-ketoacyl-(acyl-carrier-protein) reductase from Rickettsia prowazekii at 2.25 A resolution.,Subramanian S, Abendroth J, Phan IQ, Olsen C, Staker BL, Napuli A, Van Voorhis WC, Stacy R, Myler PJ Acta Crystallogr Sect F Struct Biol Cryst Commun. 2011 Sep 1;67(Pt, 9):1118-22. Epub 2011 Aug 16. PMID:21904060[1]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
See Also
References
- ↑ Subramanian S, Abendroth J, Phan IQ, Olsen C, Staker BL, Napuli A, Van Voorhis WC, Stacy R, Myler PJ. Structure of 3-ketoacyl-(acyl-carrier-protein) reductase from Rickettsia prowazekii at 2.25 A resolution. Acta Crystallogr Sect F Struct Biol Cryst Commun. 2011 Sep 1;67(Pt, 9):1118-22. Epub 2011 Aug 16. PMID:21904060 doi:10.1107/S1744309111030673
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