1gik
From Proteopedia
| Line 1: | Line 1: | ||
[[Image:1gik.gif|left|200px]] | [[Image:1gik.gif|left|200px]] | ||
| - | + | <!-- | |
| - | + | The line below this paragraph, containing "STRUCTURE_1gik", creates the "Structure Box" on the page. | |
| - | + | You may change the PDB parameter (which sets the PDB file loaded into the applet) | |
| - | + | or the SCENE parameter (which sets the initial scene displayed when the page is loaded), | |
| - | + | or leave the SCENE parameter empty for the default display. | |
| - | | | + | --> |
| - | | | + | {{STRUCTURE_1gik| PDB=1gik | SCENE= }} |
| - | + | ||
| - | + | ||
| - | }} | + | |
'''POKEWEED ANTIVIRAL PROTEIN FROM SEEDS''' | '''POKEWEED ANTIVIRAL PROTEIN FROM SEEDS''' | ||
| Line 25: | Line 22: | ||
[[Category: Phytolacca americana]] | [[Category: Phytolacca americana]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
| - | [[Category: | + | [[Category: RRNA N-glycosylase]] |
[[Category: He, X L.]] | [[Category: He, X L.]] | ||
[[Category: Hu, Z.]] | [[Category: Hu, Z.]] | ||
| Line 31: | Line 28: | ||
[[Category: Wang, D C.]] | [[Category: Wang, D C.]] | ||
[[Category: Zeng, Z H.]] | [[Category: Zeng, Z H.]] | ||
| - | [[Category: | + | [[Category: Alpha+beta]] |
| - | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May 2 17:37:01 2008'' | |
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | |
Revision as of 14:37, 2 May 2008
POKEWEED ANTIVIRAL PROTEIN FROM SEEDS
Overview
The crystal structure of pokeweed antiviral protein from seeds of Phytolacca americana (PAP-S) was solved at 1.8A. PAP-S is a one-chain ribosome-inactivating protein (RIP) and distinctively contains three well-defined N-acetylglucosamines, each covalently linked to an asparagine residue at positions, 10, 44, and 255, respectively. The high-resolution structure clearly shows the three mono-sugars to have either an alpha- or a beta-conformation. Two of sugars are located on the same side of the molecule with the active pocket. Except one hydrogen bond, there are no intermolecular interactions between the polypeptide chain and the sugars. Instead the sugar conformations appear to be stabilized by intermolecular interactions. The sugar structure defined at high resolution provides a structural basis for understanding their possible biological activity. The structural comparisons of PAP-S with other PAPs reveal that the major disparity of these homologous molecules is the different charge distribution on the upper right side of the front side near the active pocket. Based on the available structure of the 50S ribosomal subunit, the possible interactions between PAPs and the ribosome are discussed.
About this Structure
1GIK is a Single protein structure of sequence from Phytolacca americana. Full crystallographic information is available from OCA.
Reference
Crystal structure of pokeweed antiviral protein with well-defined sugars from seeds at 1.8A resolution., Zeng ZH, He XL, Li HM, Hu Z, Wang DC, J Struct Biol. 2003 Feb;141(2):171-8. PMID:12615543 Page seeded by OCA on Fri May 2 17:37:01 2008
