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1rl1

From Proteopedia

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Revision as of 06:31, 2 March 2022

Solution structure of human Sgt1 CS domain

Structural highlights

1rl1 is a 1 chain structure with sequence from Human. Full experimental information is available from OCA. For a guided tour on the structure components use FirstGlance.
Gene:	SUGT1 (HUMAN)
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

[SUGT1_HUMAN] May play a role in ubiquitination and subsequent proteasomal degradation of target proteins.

Evolutionary Conservation

Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.

Publication Abstract from PubMed

Sgt1 has been identified as a subunit of both core kinetochore and SCF (Skp1-Cul1-F-box) ubiquitin ligase complexes and is also implicated in plant disease resistance. Sgt1 has two putative HSP90 binding domains, a tetratricopeptide repeat and a p23-like CHORD and Sgt1 (CS) domain. Using NMR spectroscopy, we show that only the CS domain of human Sgt1 physically interacts with HSP90. The tetratricopeptide repeat domain does not bind to either HSP90 or HSP70. Determination of the three-dimensional structure showed that the Sgt1-CS domain shares the same beta-sandwich fold as p23 but lacks the last highly conserved beta-strand in p23. Analysis of the structures of Sgt1-CS and p23 revealed a similar charge distribution on one of two opposing surfaces that suggests that it is the binding region for HSP90 in Sgt1. Although ATP is absolutely required for p23 binding to HSP90, Sgt1 binds to HSP90 also in the absence of the non-hydrolyzable analog ATPgammaS. Our findings suggest the CS domain is a binding module for HSP90 distinct from p23-like domains, which implies that Sgt1 and related proteins function in recruiting heat shock protein activities to multiprotein assemblies.

Human Sgt1 binds HSP90 through the CHORD-Sgt1 domain and not the tetratricopeptide repeat domain.,Lee YT, Jacob J, Michowski W, Nowotny M, Kuznicki J, Chazin WJ J Biol Chem. 2004 Apr 16;279(16):16511-7. Epub 2004 Feb 3. PMID:14761955^[1]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Lee YT, Jacob J, Michowski W, Nowotny M, Kuznicki J, Chazin WJ. Human Sgt1 binds HSP90 through the CHORD-Sgt1 domain and not the tetratricopeptide repeat domain. J Biol Chem. 2004 Apr 16;279(16):16511-7. Epub 2004 Feb 3. PMID:14761955 doi:10.1074/jbc.M400215200

1 Structural highlights
2 Function
3 Evolutionary Conservation
4 Publication Abstract from PubMed
5 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/1rl1"

@@ Line 3: / Line 3: @@
 <StructureSection load='1rl1' size='340' side='right'caption='[[1rl1]], [[NMR_Ensembles_of_Models | 20 NMR models]]' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[1rl1]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Human Human]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1RL1 OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=1RL1 FirstGlance]. <br>
+<table><tr><td colspan='2'>[[1rl1]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Human Human]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1RL1 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1RL1 FirstGlance]. <br>
-</td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">SUGT1 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN])</td></tr>
+</td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">SUGT1 ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN])</td></tr>
-<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=1rl1 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1rl1 OCA], [http://pdbe.org/1rl1 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1rl1 RCSB], [http://www.ebi.ac.uk/pdbsum/1rl1 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=1rl1 ProSAT]</span></td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1rl1 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1rl1 OCA], [https://pdbe.org/1rl1 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1rl1 RCSB], [https://www.ebi.ac.uk/pdbsum/1rl1 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1rl1 ProSAT]</span></td></tr>
 </table>
 == Function ==
-[[http://www.uniprot.org/uniprot/SUGT1_HUMAN SUGT1_HUMAN]] May play a role in ubiquitination and subsequent proteasomal degradation of target proteins.
+[[https://www.uniprot.org/uniprot/SUGT1_HUMAN SUGT1_HUMAN]] May play a role in ubiquitination and subsequent proteasomal degradation of target proteins.
 == Evolutionary Conservation ==
 [[Image:Consurf_key_small.gif|200px|right]]

1rl1

From Proteopedia

Revision as of 06:31, 2 March 2022

Solution structure of human Sgt1 CS domain

Structural highlights

Function

Evolutionary Conservation

Publication Abstract from PubMed

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

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