7n5d
From Proteopedia
(Difference between revisions)
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==Composite Structure of Mechanosensitive Ion Channel Flycatcher1 in GDN== | ==Composite Structure of Mechanosensitive Ion Channel Flycatcher1 in GDN== | ||
| - | <StructureSection load='7n5d' size='340' side='right'caption='[[7n5d]]' scene=''> | + | <StructureSection load='7n5d' size='340' side='right'caption='[[7n5d]], [[Resolution|resolution]] 2.80Å' scene=''> |
== Structural highlights == | == Structural highlights == | ||
| - | <table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7N5D OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7N5D FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[7n5d]] is a 7 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7N5D OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7N5D FirstGlance]. <br> |
| - | </td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7n5d FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7n5d OCA], [https://pdbe.org/7n5d PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7n5d RCSB], [https://www.ebi.ac.uk/pdbsum/7n5d PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7n5d ProSAT]</span></td></tr> | + | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=PLM:PALMITIC+ACID'>PLM</scene></td></tr> |
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7n5d FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7n5d OCA], [https://pdbe.org/7n5d PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7n5d RCSB], [https://www.ebi.ac.uk/pdbsum/7n5d PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7n5d ProSAT]</span></td></tr> | ||
</table> | </table> | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | Flycatcher1 (FLYC1), a MscS homolog, has recently been identified as a candidate mechanosensitive (MS) ion channel involved in Venus flytrap prey recognition. FLYC1 is a larger protein and its sequence diverges from previously studied MscS homologs, suggesting it has unique structural features that contribute to its function. Here, we characterize FLYC1 by cryo-electron microscopy, molecular dynamics simulations, and electrophysiology. Akin to bacterial MscS and plant MSL1 channels, we find that FLYC1 central core includes side portals in the cytoplasmic cage that regulate ion preference and conduction, by identifying critical residues that modulate channel conductance. Topologically unique cytoplasmic flanking regions can adopt 'up' or 'down' conformations, making the channel asymmetric. Disruption of an up conformation-specific interaction severely delays channel deactivation by 40-fold likely due to stabilization of the channel open state. Our results illustrate novel structural features and likely conformational transitions that regulate mechano-gating of FLYC1. | ||
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| + | Structural insights into the Venus flytrap mechanosensitive ion channel Flycatcher1.,Jojoa-Cruz S, Saotome K, Tsui CCA, Lee WH, Sansom MSP, Murthy SE, Patapoutian A, Ward AB Nat Commun. 2022 Feb 14;13(1):850. doi: 10.1038/s41467-022-28511-5. PMID:35165281<ref>PMID:35165281</ref> | ||
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| + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | ||
| + | </div> | ||
| + | <div class="pdbe-citations 7n5d" style="background-color:#fffaf0;"></div> | ||
| + | == References == | ||
| + | <references/> | ||
__TOC__ | __TOC__ | ||
</StructureSection> | </StructureSection> | ||
[[Category: Large Structures]] | [[Category: Large Structures]] | ||
| - | [[Category: Jojoa-Cruz S]] | + | [[Category: Jojoa-Cruz, S]] |
| - | [[Category: Lee | + | [[Category: Lee, W H]] |
| - | [[Category: Patapoutian A]] | + | [[Category: Patapoutian, A]] |
| - | [[Category: Saotome K]] | + | [[Category: Saotome, K]] |
| - | [[Category: Ward | + | [[Category: Ward, A B]] |
| + | [[Category: Mechanically activated ion channel]] | ||
| + | [[Category: Membrane protein]] | ||
Revision as of 07:28, 2 March 2022
Composite Structure of Mechanosensitive Ion Channel Flycatcher1 in GDN
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