7vh3
From Proteopedia
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==Cryo-EM structure of Machupo virus polymerase L== | ==Cryo-EM structure of Machupo virus polymerase L== | ||
| - | <StructureSection load='7vh3' size='340' side='right'caption='[[7vh3]]' scene=''> | + | <StructureSection load='7vh3' size='340' side='right'caption='[[7vh3]], [[Resolution|resolution]] 3.60Å' scene=''> |
== Structural highlights == | == Structural highlights == | ||
| - | <table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7VH3 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7VH3 FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[7vh3]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7VH3 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7VH3 FirstGlance]. <br> |
| - | </td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7vh3 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7vh3 OCA], [https://pdbe.org/7vh3 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7vh3 RCSB], [https://www.ebi.ac.uk/pdbsum/7vh3 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7vh3 ProSAT]</span></td></tr> | + | </td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[7vh2|7vh2]], [[7vh1|7vh1]], [[7vgq|7vgq]]</div></td></tr> |
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7vh3 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7vh3 OCA], [https://pdbe.org/7vh3 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7vh3 RCSB], [https://www.ebi.ac.uk/pdbsum/7vh3 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7vh3 ProSAT]</span></td></tr> | ||
</table> | </table> | ||
| + | == Function == | ||
| + | [[https://www.uniprot.org/uniprot/L_MACHU L_MACHU]] RNA-dependent RNA polymerase which is responsible for replication and transcription of the viral RNA genome. During transcription, synthesizes 4 subgenomic RNAs, and assures their capping by a cap-snatching mechanism, in which cellular capped pre-mRNAs are used to generate primers for viral transcription. The 3'-end of subgenomic mRNAs molecules are heterogeneous and not polyadenylated. The replicase function is to direct synthesis of antigenomic and genomic RNA which are encapsidated and non capped. As a consequence of the use of the same enzyme for both transcription and replication, these mechanisms need to be well coordinated. These processes may be regulated by proteins N and Z in a dose-dependent manner. | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | The Arenaviridae family includes several viruses that cause severe human hemorrhagic fevers with high mortality, with no effective countermeasures currently available. The arenavirus multi-domain L protein is involved in viral transcription and replication and represents a promising target for antiviral drugs. The arenavirus matrix protein Z is a small multi-functional protein that inhibits the activities of the L protein. Here we report the structure of Machupo virus L protein in complex with Z determined by cryo-electron microscopy. The Z protein acts as a staple and binds the L protein with 1:1 stoichiometry at the intersection between the PA-C-like region, RNA-dependent RNA polymerase and PB2-N-like region. Binding of the Z protein may lock the multiple domains of L into a fixed arrangement leading to loss of catalytic activity. These results further our understanding of the inhibitory mechanism of arenavirus replication machinery and provide a novel perspective to develop antiviral drugs. | ||
| + | |||
| + | Structure of Machupo virus polymerase in complex with matrix protein Z.,Ma J, Zhang S, Zhang X Nat Commun. 2021 Oct 25;12(1):6163. doi: 10.1038/s41467-021-26432-3. PMID:34697302<ref>PMID:34697302</ref> | ||
| + | |||
| + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | ||
| + | </div> | ||
| + | <div class="pdbe-citations 7vh3" style="background-color:#fffaf0;"></div> | ||
| + | == References == | ||
| + | <references/> | ||
__TOC__ | __TOC__ | ||
</StructureSection> | </StructureSection> | ||
[[Category: Large Structures]] | [[Category: Large Structures]] | ||
| - | [[Category: Ma J]] | + | [[Category: Ma, J]] |
| - | [[Category: Zhang S]] | + | [[Category: Zhang, S]] |
| - | [[Category: Zhang X]] | + | [[Category: Zhang, X]] |
| + | [[Category: Polymerase]] | ||
| + | [[Category: Viral protein]] | ||
Revision as of 07:33, 2 March 2022
Cryo-EM structure of Machupo virus polymerase L
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