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| | ==Recognition of AT-rich DNA binding sites by the MogR Repressor== | | ==Recognition of AT-rich DNA binding sites by the MogR Repressor== |
| - | <StructureSection load='3fdq' size='340' side='right' caption='[[3fdq]], [[Resolution|resolution]] 1.75Å' scene=''> | + | <StructureSection load='3fdq' size='340' side='right'caption='[[3fdq]], [[Resolution|resolution]] 1.75Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[3fdq]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/"bacterium_monocytogenes_hominis"_nyfeldt_1932 "bacterium monocytogenes hominis" nyfeldt 1932]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3FDQ OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3FDQ FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[3fdq]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/"bacterium_monocytogenes_hominis"_nyfeldt_1932 "bacterium monocytogenes hominis" nyfeldt 1932]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3FDQ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3FDQ FirstGlance]. <br> |
| - | </td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">lmo0674, mogR ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1639 "Bacterium monocytogenes hominis" Nyfeldt 1932])</td></tr> | + | </td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">lmo0674, mogR ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1639 "Bacterium monocytogenes hominis" Nyfeldt 1932])</td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3fdq FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3fdq OCA], [http://pdbe.org/3fdq PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3fdq RCSB], [http://www.ebi.ac.uk/pdbsum/3fdq PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=3fdq ProSAT]</span></td></tr> | + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3fdq FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3fdq OCA], [https://pdbe.org/3fdq PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3fdq RCSB], [https://www.ebi.ac.uk/pdbsum/3fdq PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3fdq ProSAT]</span></td></tr> |
| | </table> | | </table> |
| | == Function == | | == Function == |
| - | [[http://www.uniprot.org/uniprot/MOGR_LISMO MOGR_LISMO]] Transcriptional repressor of flagellar motility genes, such as flaA, during extracellular growth at 37 degrees Celsius and during intracellular infection. Binds directly to the gene promoter region and probably prevents RNA polymerase binding. At low temperatures, MogR repression activity is modulated by the DegU response regulator in an unknown mechanism. Required for full virulence.<ref>PMID:15302931</ref> <ref>PMID:16617375</ref> | + | [[https://www.uniprot.org/uniprot/MOGR_LISMO MOGR_LISMO]] Transcriptional repressor of flagellar motility genes, such as flaA, during extracellular growth at 37 degrees Celsius and during intracellular infection. Binds directly to the gene promoter region and probably prevents RNA polymerase binding. At low temperatures, MogR repression activity is modulated by the DegU response regulator in an unknown mechanism. Required for full virulence.<ref>PMID:15302931</ref> <ref>PMID:16617375</ref> |
| | == Evolutionary Conservation == | | == Evolutionary Conservation == |
| | [[Image:Consurf_key_small.gif|200px|right]] | | [[Image:Consurf_key_small.gif|200px|right]] |
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| | </StructureSection> | | </StructureSection> |
| | [[Category: Bacterium monocytogenes hominis nyfeldt 1932]] | | [[Category: Bacterium monocytogenes hominis nyfeldt 1932]] |
| | + | [[Category: Large Structures]] |
| | [[Category: Higgins, D E]] | | [[Category: Higgins, D E]] |
| | [[Category: Panne, D]] | | [[Category: Panne, D]] |
| Structural highlights
Function
[MOGR_LISMO] Transcriptional repressor of flagellar motility genes, such as flaA, during extracellular growth at 37 degrees Celsius and during intracellular infection. Binds directly to the gene promoter region and probably prevents RNA polymerase binding. At low temperatures, MogR repression activity is modulated by the DegU response regulator in an unknown mechanism. Required for full virulence.[1] [2]
Evolutionary Conservation
Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.
Publication Abstract from PubMed
The MogR transcriptional repressor of the intracellular pathogen Listeria monocytogenes recognizes AT-rich binding sites in promoters of flagellar genes to downregulate flagellar gene expression during infection. We describe here the 1.8 A resolution crystal structure of MogR bound to the recognition sequence 5' ATTTTTTAAAAAAAT 3' present within the flaA promoter region. Our structure shows that MogR binds as a dimer. Each half-site is recognized in the major groove by a helix-turn-helix motif and in the minor groove by a loop from the symmetry-related molecule, resulting in a "crossover" binding mode. This oversampling through minor groove interactions is important for specificity. The MogR binding site has structural features of A-tract DNA and is bent by approximately 52 degrees away from the dimer. The structure explains how MogR achieves binding specificity in the AT-rich genome of L. monocytogenes and explains the evolutionary conservation of A-tract sequence elements within promoter regions of MogR-regulated flagellar genes.
Recognition of AT-rich DNA binding sites by the MogR repressor.,Shen A, Higgins DE, Panne D Structure. 2009 May 13;17(5):769-77. PMID:19446532[3]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ Grundling A, Burrack LS, Bouwer HG, Higgins DE. Listeria monocytogenes regulates flagellar motility gene expression through MogR, a transcriptional repressor required for virulence. Proc Natl Acad Sci U S A. 2004 Aug 17;101(33):12318-23. Epub 2004 Aug 9. PMID:15302931 doi:http://dx.doi.org/10.1073/pnas.0404924101
- ↑ Shen A, Higgins DE. The MogR transcriptional repressor regulates nonhierarchal expression of flagellar motility genes and virulence in Listeria monocytogenes. PLoS Pathog. 2006 Apr;2(4):e30. Epub 2006 Apr 14. PMID:16617375 doi:http://dx.doi.org/10.1371/journal.ppat.0020030
- ↑ Shen A, Higgins DE, Panne D. Recognition of AT-rich DNA binding sites by the MogR repressor. Structure. 2009 May 13;17(5):769-77. PMID:19446532 doi:10.1016/j.str.2009.02.018
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