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3fpz
From Proteopedia
(Difference between revisions)
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==Saccharomyces cerevisiae THI4p is a suicide thiamin thiazole synthase== | ==Saccharomyces cerevisiae THI4p is a suicide thiamin thiazole synthase== | ||
| - | <StructureSection load='3fpz' size='340' side='right' caption='[[3fpz]], [[Resolution|resolution]] 1.82Å' scene=''> | + | <StructureSection load='3fpz' size='340' side='right'caption='[[3fpz]], [[Resolution|resolution]] 1.82Å' scene=''> |
== Structural highlights == | == Structural highlights == | ||
| - | <table><tr><td colspan='2'>[[3fpz]] is a 2 chain structure with sequence from [ | + | <table><tr><td colspan='2'>[[3fpz]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Atcc_18824 Atcc 18824]. This structure supersedes the now removed PDB entry [http://oca.weizmann.ac.il/oca-bin/send-pdb?obs=1&id=2gjc 2gjc]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3FPZ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3FPZ FirstGlance]. <br> |
| - | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=AHZ:ADENOSINE+DIPHOSPHATE+5-(BETA-ETHYL)-4-METHYL-THIAZOLE-2-CARBOXYLIC+ACID'>AHZ</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr> | + | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=AHZ:ADENOSINE+DIPHOSPHATE+5-(BETA-ETHYL)-4-METHYL-THIAZOLE-2-CARBOXYLIC+ACID'>AHZ</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr> |
<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=DHA:2-AMINO-ACRYLIC+ACID'>DHA</scene></td></tr> | <tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=DHA:2-AMINO-ACRYLIC+ACID'>DHA</scene></td></tr> | ||
| - | <tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[1rp0|1rp0]], [[2gjc|2gjc]]</td></tr> | + | <tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[1rp0|1rp0]], [[2gjc|2gjc]]</div></td></tr> |
| - | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">THI4, ESP35, MOL1, YGR144W, G6620 ([ | + | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">THI4, ESP35, MOL1, YGR144W, G6620 ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=4932 ATCC 18824])</td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[ | + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3fpz FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3fpz OCA], [https://pdbe.org/3fpz PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3fpz RCSB], [https://www.ebi.ac.uk/pdbsum/3fpz PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3fpz ProSAT]</span></td></tr> |
</table> | </table> | ||
== Function == | == Function == | ||
| - | [[ | + | [[https://www.uniprot.org/uniprot/THI4_YEAST THI4_YEAST]] Involved in biosynthesis of the thiamine precursor thiazole. Catalyzes the conversion of NAD and glycine to adenosine diphosphate 5-(2-hydroxyethyl)-4-methylthiazole-2-carboxylic acid (ADT), an adenylated thiazole intermediate. The reaction includes an iron-dependent sulfide transfer from a conserved cysteine residue of the protein to a thiazole intermediate. The enzyme can only undergo a single turnover, which suggests it is a suicide enzyme. May have additional roles in adaptation to various stress conditions and in DNA damage tolerance.<ref>PMID:15544818</ref> <ref>PMID:9367751</ref> <ref>PMID:16734458</ref> <ref>PMID:16171982</ref> <ref>PMID:17309261</ref> <ref>PMID:18652458</ref> <ref>PMID:22031445</ref> |
== Evolutionary Conservation == | == Evolutionary Conservation == | ||
[[Image:Consurf_key_small.gif|200px|right]] | [[Image:Consurf_key_small.gif|200px|right]] | ||
Check<jmol> | Check<jmol> | ||
<jmolCheckbox> | <jmolCheckbox> | ||
| - | <scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/fp/3fpz_consurf.spt"</scriptWhenChecked> | + | <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/fp/3fpz_consurf.spt"</scriptWhenChecked> |
<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked> | <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked> | ||
<text>to colour the structure by Evolutionary Conservation</text> | <text>to colour the structure by Evolutionary Conservation</text> | ||
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</StructureSection> | </StructureSection> | ||
[[Category: Atcc 18824]] | [[Category: Atcc 18824]] | ||
| + | [[Category: Large Structures]] | ||
[[Category: Bale, S]] | [[Category: Bale, S]] | ||
[[Category: Begley, T P]] | [[Category: Begley, T P]] | ||
Revision as of 08:07, 2 March 2022
Saccharomyces cerevisiae THI4p is a suicide thiamin thiazole synthase
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