1gk4
From Proteopedia
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'''HUMAN VIMENTIN COIL 2B FRAGMENT (CYS2)''' | '''HUMAN VIMENTIN COIL 2B FRAGMENT (CYS2)''' | ||
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[[Category: Strelkov, S V.]] | [[Category: Strelkov, S V.]] | ||
[[Category: Zimbelmann, R.]] | [[Category: Zimbelmann, R.]] | ||
| - | [[Category: | + | [[Category: Dimer]] |
| - | [[Category: | + | [[Category: Heptad repeat]] |
| - | [[Category: | + | [[Category: Intermediate filament]] |
| - | [[Category: | + | [[Category: Parallel coiled coil]] |
| - | [[Category: | + | [[Category: Stutter]] |
| - | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May 2 17:40:42 2008'' | |
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | |
Revision as of 14:40, 2 May 2008
HUMAN VIMENTIN COIL 2B FRAGMENT (CYS2)
Overview
Intermediate filaments (IFs) are key components of the cytoskeleton in higher eukaryotic cells. The elementary IF 'building block' is an elongated coiled-coil dimer consisting of four consecutive alpha-helical segments. The segments 1A and 2B include highly conserved sequences and are critically involved in IF assembly. Based on the crystal structures of three human vimentin fragments at 1.4-2.3 A resolution (PDB entries 1gk4, 1gk6 and 1gk7), we have established the molecular organization of these two segments. The fragment corresponding to segment 1A forms a single, amphipatic alpha-helix, which is compatible with a coiled-coil geometry. While this segment might yield a coiled coil within an isolated dimer, monomeric 1A helices are likely to play a role in specific dimer-dimer interactions during IF assembly. The 2B segment reveals a double-stranded coiled coil, which unwinds near residue Phe351 to accommodate a 'stutter'. A fragment containing the last seven heptads of 2B interferes heavily with IF assembly and also transforms mature vimentin filaments into a new kind of structure. These results provide the first insight into the architecture and functioning of IFs at the atomic level.
About this Structure
1GK4 is a Single protein structure of sequence from Homo sapiens. Full crystallographic information is available from OCA.
Reference
Conserved segments 1A and 2B of the intermediate filament dimer: their atomic structures and role in filament assembly., Strelkov SV, Herrmann H, Geisler N, Wedig T, Zimbelmann R, Aebi U, Burkhard P, EMBO J. 2002 Mar 15;21(6):1255-66. PMID:11889032 Page seeded by OCA on Fri May 2 17:40:42 2008
