1gkm

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[[Image:1gkm.gif|left|200px]]
[[Image:1gkm.gif|left|200px]]
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{{Structure
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<!--
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|PDB= 1gkm |SIZE=350|CAPTION= <scene name='initialview01'>1gkm</scene>, resolution 1.0&Aring;
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The line below this paragraph, containing "STRUCTURE_1gkm", creates the "Structure Box" on the page.
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|SITE= <scene name='pdbsite=MIO:Modification+Forming+The+Catalytically+Essential+Electro+...'>MIO</scene>
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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|LIGAND= <scene name='pdbligand=CYS:CYSTEINE'>CYS</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=O:OXYGEN+ATOM'>O</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene>
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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|ACTIVITY= <span class='plainlinks'>[http://en.wikipedia.org/wiki/Histidine_ammonia-lyase Histidine ammonia-lyase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=4.3.1.3 4.3.1.3] </span>
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or leave the SCENE parameter empty for the default display.
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|GENE=
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-->
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|DOMAIN=
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{{STRUCTURE_1gkm| PDB=1gkm | SCENE= }}
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|RELATEDENTRY=
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|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1gkm FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1gkm OCA], [http://www.ebi.ac.uk/pdbsum/1gkm PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1gkm RCSB]</span>
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}}
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'''HISTIDINE AMMONIA-LYASE (HAL) FROM PSEUDOMONAS PUTIDA INHIBITED WITH L-CYSTEINE'''
'''HISTIDINE AMMONIA-LYASE (HAL) FROM PSEUDOMONAS PUTIDA INHIBITED WITH L-CYSTEINE'''
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[[Category: Baedeker, M.]]
[[Category: Baedeker, M.]]
[[Category: Schulz, G E.]]
[[Category: Schulz, G E.]]
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[[Category: ammonia-lyase]]
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[[Category: Ammonia-lyase]]
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[[Category: histidine degradation]]
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[[Category: Histidine degradation]]
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[[Category: lyase]]
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[[Category: Lyase]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May 2 17:41:51 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 30 20:46:09 2008''
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Revision as of 14:41, 2 May 2008

Image:1gkm.gif

Template:STRUCTURE 1gkm

HISTIDINE AMMONIA-LYASE (HAL) FROM PSEUDOMONAS PUTIDA INHIBITED WITH L-CYSTEINE


Overview

Histidine ammonia-lyase (EC 4.3.1.3) catalyzes the nonoxidative elimination of the alpha-amino group of histidine using a 4-methylidene-imidazole-5-one (MIO), which is formed autocatalytically from the internal peptide segment 142Ala-Ser-Gly. The structure of the enzyme inhibited by a reaction with l-cysteine was established at the very high resolution of 1.0 A. Five active center mutants were produced and their catalytic activities were measured. Among them, mutant Tyr280-->Phe could be crystallized and its structure could be determined at 1.7 A resolution. It contains a planar sp2-hybridized 144-N atom of MIO, in contrast to the pyramidal sp3-hybridized 144-N of the wild-type. With the planar 144-N atom, MIO assumes the conformation of a putative intermediate aromatic state of the reaction, demonstrating that the conformational barrier between aromatic and wild-type states is very low. The data led to a new proposal for the geometry for the catalyzed reaction, which also applies to the closely related phenylalanine ammonia-lyase (EC 4.3.1.5). Moreover, it suggested an intermediate binding site for the released ammonia.

About this Structure

1GKM is a Single protein structure of sequence from Pseudomonas putida. Full crystallographic information is available from OCA.

Reference

Structures of two histidine ammonia-lyase modifications and implications for the catalytic mechanism., Baedeker M, Schulz GE, Eur J Biochem. 2002 Mar;269(6):1790-7. PMID:11895450 Page seeded by OCA on Fri May 2 17:41:51 2008

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