7pe1
From Proteopedia
(Difference between revisions)
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==Cryo-EM structure of BMV-derived VLP expressed in E. coli and assembled in the presence of tRNA (tVLP)== | ==Cryo-EM structure of BMV-derived VLP expressed in E. coli and assembled in the presence of tRNA (tVLP)== | ||
| - | <StructureSection load='7pe1' size='340' side='right'caption='[[7pe1]]' scene=''> | + | <StructureSection load='7pe1' size='340' side='right'caption='[[7pe1]], [[Resolution|resolution]] 3.00Å' scene=''> |
== Structural highlights == | == Structural highlights == | ||
| - | <table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7PE1 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7PE1 FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[7pe1]] is a 180 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7PE1 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7PE1 FirstGlance]. <br> |
</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7pe1 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7pe1 OCA], [https://pdbe.org/7pe1 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7pe1 RCSB], [https://www.ebi.ac.uk/pdbsum/7pe1 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7pe1 ProSAT]</span></td></tr> | </td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7pe1 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7pe1 OCA], [https://pdbe.org/7pe1 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7pe1 RCSB], [https://www.ebi.ac.uk/pdbsum/7pe1 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7pe1 ProSAT]</span></td></tr> | ||
</table> | </table> | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | The increasing interest in virus-like particles (VLPs) has been reflected by the growing number of studies on their assembly and application. However, the formation of complete VLPs is a complex phenomenon, making it difficult to rationally design VLPs with desired features de novo. In this paper, we describe VLPs assembled in vitro from the recombinant capsid protein of brome mosaic virus (BMV). The analysis of VLPs was performed by Cryo-EM reconstructions and allowed us to visualize a few classes of VLPs, giving insight into the VLP self-assembly process. Apart from the mature icosahedral VLP practically identical with native virions, we describe putative VLP intermediates displaying non-icosahedral arrangements of capsomers, proposed to occur before the final disorder-order transition stage of icosahedral VLP assembly. Some of the described VLP classes show a lack of protein shell continuity, possibly resulting from too strong interaction with the cargo (in this case tRNA) with the capsid protein. We believe that our results are a useful prerequisite for the rational design of VLPs in the future and lead the way to the effective production of modified VLPs. | ||
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| + | Cryo-EM reconstructions of BMV-derived virus-like particles reveal assembly defects in the icosahedral lattice structure.,Ruszkowski M, Strugala A, Indyka P, Tresset G, Figlerowicz M, Urbanowicz A Nanoscale. 2022 Feb 24;14(8):3224-3233. doi: 10.1039/d1nr05650f. PMID:35156989<ref>PMID:35156989</ref> | ||
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| + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | ||
| + | </div> | ||
| + | <div class="pdbe-citations 7pe1" style="background-color:#fffaf0;"></div> | ||
| + | == References == | ||
| + | <references/> | ||
__TOC__ | __TOC__ | ||
</StructureSection> | </StructureSection> | ||
[[Category: Large Structures]] | [[Category: Large Structures]] | ||
| - | [[Category: Indyka P]] | + | [[Category: Indyka, P]] |
| - | [[Category: Ruszkowski M]] | + | [[Category: Ruszkowski, M]] |
| - | [[Category: Strugala A]] | + | [[Category: Strugala, A]] |
| - | [[Category: Urbanowicz A]] | + | [[Category: Urbanowicz, A]] |
| + | [[Category: Bmv]] | ||
| + | [[Category: Brome mosaic virus]] | ||
| + | [[Category: Capsid protein]] | ||
| + | [[Category: Virus like particle]] | ||
Revision as of 07:24, 9 March 2022
Cryo-EM structure of BMV-derived VLP expressed in E. coli and assembled in the presence of tRNA (tVLP)
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