1gm6
From Proteopedia
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'''3-D STRUCTURE OF A SALIVARY LIPOCALIN FROM BOAR''' | '''3-D STRUCTURE OF A SALIVARY LIPOCALIN FROM BOAR''' | ||
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[[Category: Tegoni, M.]] | [[Category: Tegoni, M.]] | ||
[[Category: Vincent, F.]] | [[Category: Vincent, F.]] | ||
| - | [[Category: | + | [[Category: Odorant binding protein]] |
| - | [[Category: | + | [[Category: Salivary lipocalin]] |
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Revision as of 14:44, 2 May 2008
3-D STRUCTURE OF A SALIVARY LIPOCALIN FROM BOAR
Overview
The X-ray structure of variant A of authentic boar salivary lipocalin (SAL), a pheromone-binding protein specifically expressed in the submaxillary glands of the boar, has been solved and refined at 2.1 A resolution. The structure displays a classical lipocalin fold with a nine-stranded sandwiched beta barrel and an alpha helix. A putative glycosylation site, at position 53, has been found to carry a GlcNAc sugar residue. In contrast with what was expected on the basis of mass spectroscopy reports, the internal cavity was found to be devoid of bound pheromonal compound (androstenone or androstenol). Instead, a small electron density volume could be satisfied by a glycerol molecule, a component of the cryoprotecting liquor. The internal cavity was revealed to be very small for steroid compound accommodation. Therefore, docking and molecular dynamics experiments were performed with both pheromonal compounds. These simulations clearly demonstrate a volume increase of the cavity upon steroid binding and the adaptation of the amino-acid side chains to the steroid molecules. This explains the higher affinity of SAL for both steroid molecules compared to other smaller molecules, although no specific interaction is established with either compound.
About this Structure
1GM6 is a Single protein structure of sequence from Sus scrofa. Full crystallographic information is available from OCA.
Reference
Boar salivary lipocalin. Three-dimensional X-ray structure and androsterol/androstenone docking simulations., Spinelli S, Vincent F, Pelosi P, Tegoni M, Cambillau C, Eur J Biochem. 2002 May;269(10):2449-56. PMID:12027882 Page seeded by OCA on Fri May 2 17:44:37 2008
