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6c8u

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Solution structure of Musashi2 RRM1

Structural highlights

6c8u is a 1 chain structure with sequence from Human. Full experimental information is available from OCA. For a guided tour on the structure components use FirstGlance.
Gene:	MSI2 (HUMAN)
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Disease

[MSI2H_HUMAN] Chromosomal aberrations involving MSI2 may contribute to disease progression in chronic myeloid leukemia. Translocation t(7;17)(p15;q23) with HOXA9; translocation t(7;17)(q32-34;q23).^[1]

Function

[MSI2H_HUMAN] RNA binding protein that regulates the expression of target mRNAs at the translation level. May play a role in the proliferation and maintenance of stem cells in the central nervous system (By similarity).

Publication Abstract from PubMed

Musashi-2 (MSI2) belongs to Musashi family of RNA binding proteins. Like Musashi-1 (MSI1), it is overexpressed in a variety of cancers and is a promising therapeutic target. Both MSI proteins contain two N-terminal RNA recognition motifs and play roles in post-transcriptional regulation of target mRNAs. Previously, we have identified several inhibitors of MSI1, all of which bind to MSI2 as well. In order to design MSI2 specific inhibitors and compare the differences of binding mode of the inhibitors, we set out to solve the structure of MSI2-RRM1, the key motif that is responsible for the binding. Here we report the crystal structure and the first NMR solution structure of MSI2-RRM1, and compare these to the structures of MSI1-RBD1 and other RNA binding proteins. A high degree of structural similarity was observed between the crystal and solution NMR structures. MSI2-RRM1 shows a highly similar overall folding topology to MSI1-RBD1 and other RNA binding proteins. The structural information of MSI2-RRM1 will be helpful for understanding MSI2-RNA interaction and for guiding rational drug design of MSI2 specific inhibitors. This article is protected by copyright. All rights reserved.

Crystal and Solution Structures of Human Oncoprotein Musashi-2 N-terminal RNA Recognition Motif 1.,Lan L, Xing M, Kashipathy MM, Douglas JT, Gao P, Battaile KP, Hanzlik RP, Lovell S, Xu L Proteins. 2019 Oct 11. doi: 10.1002/prot.25836. PMID:31603583^[2]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Barbouti A, Hoglund M, Johansson B, Lassen C, Nilsson PG, Hagemeijer A, Mitelman F, Fioretos T. A novel gene, MSI2, encoding a putative RNA-binding protein is recurrently rearranged at disease progression of chronic myeloid leukemia and forms a fusion gene with HOXA9 as a result of the cryptic t(7;17)(p15;q23). Cancer Res. 2003 Mar 15;63(6):1202-6. PMID:12649177
↑ Lan L, Xing M, Kashipathy MM, Douglas JT, Gao P, Battaile KP, Hanzlik RP, Lovell S, Xu L. Crystal and Solution Structures of Human Oncoprotein Musashi-2 N-terminal RNA Recognition Motif 1. Proteins. 2019 Oct 11. doi: 10.1002/prot.25836. PMID:31603583 doi:http://dx.doi.org/10.1002/prot.25836

1 Structural highlights
2 Disease
3 Function
4 Publication Abstract from PubMed
5 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/6c8u"

@@ Line 3: / Line 3: @@
 <StructureSection load='6c8u' size='340' side='right'caption='[[6c8u]], [[NMR_Ensembles_of_Models | 10 NMR models]]' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[6c8u]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Human Human]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6C8U OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6C8U FirstGlance]. <br>
+<table><tr><td colspan='2'>[[6c8u]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Human Human]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6C8U OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6C8U FirstGlance]. <br>
-</td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">MSI2 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN])</td></tr>
+</td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">MSI2 ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN])</td></tr>
-<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6c8u FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6c8u OCA], [http://pdbe.org/6c8u PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6c8u RCSB], [http://www.ebi.ac.uk/pdbsum/6c8u PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6c8u ProSAT]</span></td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6c8u FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6c8u OCA], [https://pdbe.org/6c8u PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6c8u RCSB], [https://www.ebi.ac.uk/pdbsum/6c8u PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6c8u ProSAT]</span></td></tr>
 </table>
 == Disease ==
-[[http://www.uniprot.org/uniprot/MSI2H_HUMAN MSI2H_HUMAN]] Chromosomal aberrations involving MSI2 may contribute to disease progression in chronic myeloid leukemia. Translocation t(7;17)(p15;q23) with HOXA9; translocation t(7;17)(q32-34;q23).<ref>PMID:12649177</ref>
+[[https://www.uniprot.org/uniprot/MSI2H_HUMAN MSI2H_HUMAN]] Chromosomal aberrations involving MSI2 may contribute to disease progression in chronic myeloid leukemia. Translocation t(7;17)(p15;q23) with HOXA9; translocation t(7;17)(q32-34;q23).<ref>PMID:12649177</ref>
 == Function ==
-[[http://www.uniprot.org/uniprot/MSI2H_HUMAN MSI2H_HUMAN]] RNA binding protein that regulates the expression of target mRNAs at the translation level. May play a role in the proliferation and maintenance of stem cells in the central nervous system (By similarity).
+[[https://www.uniprot.org/uniprot/MSI2H_HUMAN MSI2H_HUMAN]] RNA binding protein that regulates the expression of target mRNAs at the translation level. May play a role in the proliferation and maintenance of stem cells in the central nervous system (By similarity).
 <div style="background-color:#fffaf0;">
 == Publication Abstract from PubMed ==

6c8u

From Proteopedia

Current revision

Solution structure of Musashi2 RRM1

Structural highlights

Disease

Function

Publication Abstract from PubMed

References

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