7f2j
From Proteopedia
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==Crystal structure of AtFKBP53 FKBD in complex with rapamycin== | ==Crystal structure of AtFKBP53 FKBD in complex with rapamycin== | ||
| - | <StructureSection load='7f2j' size='340' side='right'caption='[[7f2j]]' scene=''> | + | <StructureSection load='7f2j' size='340' side='right'caption='[[7f2j]], [[Resolution|resolution]] 1.60Å' scene=''> |
== Structural highlights == | == Structural highlights == | ||
| - | <table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7F2J OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7F2J FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[7f2j]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7F2J OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7F2J FirstGlance]. <br> |
| - | </td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7f2j FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7f2j OCA], [https://pdbe.org/7f2j PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7f2j RCSB], [https://www.ebi.ac.uk/pdbsum/7f2j PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7f2j ProSAT]</span></td></tr> | + | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=PEG:DI(HYDROXYETHYL)ETHER'>PEG</scene>, <scene name='pdbligand=RAP:RAPAMYCIN+IMMUNOSUPPRESSANT+DRUG'>RAP</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr> |
| + | <tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[6j2m|6j2m]]</div></td></tr> | ||
| + | <tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[https://en.wikipedia.org/wiki/Peptidylprolyl_isomerase Peptidylprolyl isomerase], with EC number [https://www.brenda-enzymes.info/php/result_flat.php4?ecno=5.2.1.8 5.2.1.8] </span></td></tr> | ||
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7f2j FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7f2j OCA], [https://pdbe.org/7f2j PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7f2j RCSB], [https://www.ebi.ac.uk/pdbsum/7f2j PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7f2j ProSAT]</span></td></tr> | ||
</table> | </table> | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | Chemically induced dimerization (CID) is used to induce proximity and result in artificial complex formation between a pair of proteins involved in biological processes in cells to investigate and regulate these processes. The induced heterodimerization of FKBP fusion proteins by rapamycin and FK506 has been extensively exploited as a chemically induced dimerization system to regulate and understand highly dynamic cellular processes. Here, we report the crystal structure of the AtFKBP53 FKBD in complex with rapamycin. The crystal packing reveals an unusual feature whereby two rapamycin molecules appear to mediate homodimerization of the FKBD. The triene arm of rapamycin appears to play a significant role in forming this dimer. This forms the first structural report of rapamycin-mediated homodimerization of an FKBP. The structural information on the rapamycin-mediated FKBD dimerization may be employed to design and synthesize covalently linked dimeric rapamycin, which may subsequently serve as a chemically induced dimerization system for the regulation and characterization of cellular processes. | ||
| + | |||
| + | Crystal packing reveals rapamycin-mediated homodimerization of an FK506-binding domain.,Singh AK, Saharan K, Baral S, Luan S, Vasudevan D Int J Biol Macromol. 2022 Feb 23. pii: S0141-8130(22)00354-3. doi:, 10.1016/j.ijbiomac.2022.02.107. PMID:35218805<ref>PMID:35218805</ref> | ||
| + | |||
| + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | ||
| + | </div> | ||
| + | <div class="pdbe-citations 7f2j" style="background-color:#fffaf0;"></div> | ||
| + | == References == | ||
| + | <references/> | ||
__TOC__ | __TOC__ | ||
</StructureSection> | </StructureSection> | ||
[[Category: Large Structures]] | [[Category: Large Structures]] | ||
| - | [[Category: Saharan K]] | + | [[Category: Peptidylprolyl isomerase]] |
| - | [[Category: Singh | + | [[Category: Saharan, K]] |
| - | [[Category: Vasudevan D]] | + | [[Category: Singh, A K]] |
| + | [[Category: Vasudevan, D]] | ||
| + | [[Category: Dimerization]] | ||
| + | [[Category: Fkbp]] | ||
| + | [[Category: Isomerase]] | ||
| + | [[Category: Rapamycin]] | ||
Revision as of 11:54, 23 March 2022
Crystal structure of AtFKBP53 FKBD in complex with rapamycin
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