7npq
From Proteopedia
(Difference between revisions)
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==Crystal structure of the human LL37(17-29) I24C mutant antimicrobial peptide== | ==Crystal structure of the human LL37(17-29) I24C mutant antimicrobial peptide== | ||
| - | <StructureSection load='7npq' size='340' side='right'caption='[[7npq]]' scene=''> | + | <StructureSection load='7npq' size='340' side='right'caption='[[7npq]], [[Resolution|resolution]] 1.50Å' scene=''> |
== Structural highlights == | == Structural highlights == | ||
| - | <table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7NPQ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7NPQ FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[7npq]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7NPQ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7NPQ FirstGlance]. <br> |
</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7npq FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7npq OCA], [https://pdbe.org/7npq PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7npq RCSB], [https://www.ebi.ac.uk/pdbsum/7npq PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7npq ProSAT]</span></td></tr> | </td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7npq FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7npq OCA], [https://pdbe.org/7npq PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7npq RCSB], [https://www.ebi.ac.uk/pdbsum/7npq PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7npq ProSAT]</span></td></tr> | ||
</table> | </table> | ||
| + | == Function == | ||
| + | [[https://www.uniprot.org/uniprot/CAMP_HUMAN CAMP_HUMAN]] Binds to bacterial lipopolysaccharides (LPS), has antibacterial activity.<ref>PMID:16637646</ref> <ref>PMID:18818205</ref> | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | Human LL-3717-29 is an antimicrobial peptide forming thermostable supramolecular fibrils that surround bacterial cells. The crystal structure of LL-3717-29 bearing an I24C substitution of most buried position in the fibril revealed disulfide-bonded dimers that further assembled into a fibrillar structure of densely packed helices. We further demonstrated the position-dependent controllable antibacterial activity of LL-3717-29 I24C and other cysteine mutants, mediated by regulation of intermolecular disulfide bonds and their role in the formation of supramolecular structures. The morphology of the fibrils and their antibacterial mechanism of action might be dependent on their interactions with specific bacteria. The significant effect of disulfide bonds on the assembly into supramolecular structures and their sensitivity to reducing/oxidizing conditions may explain why short helical antimicrobial peptides with a single cysteine and an odd number of cysteines are selected against in nature. | ||
| + | |||
| + | Rare by Natural Selection: Disulfide-Bonded Supramolecular Antimicrobial Peptides.,Engelberg Y, Ragonis-Bachar P, Landau M Biomacromolecules. 2022 Jan 21. doi: 10.1021/acs.biomac.1c01353. PMID:35061360<ref>PMID:35061360</ref> | ||
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| + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | ||
| + | </div> | ||
| + | <div class="pdbe-citations 7npq" style="background-color:#fffaf0;"></div> | ||
| + | == References == | ||
| + | <references/> | ||
__TOC__ | __TOC__ | ||
</StructureSection> | </StructureSection> | ||
[[Category: Large Structures]] | [[Category: Large Structures]] | ||
| - | [[Category: Engelberg Y]] | + | [[Category: Engelberg, Y]] |
| - | [[Category: Landau M]] | + | [[Category: Landau, M]] |
| + | [[Category: Amp]] | ||
| + | [[Category: Antimicrobial peptide]] | ||
| + | [[Category: Antimicrobial protein]] | ||
| + | [[Category: Cys mutant]] | ||
| + | [[Category: Design]] | ||
| + | [[Category: Functional fibril]] | ||
| + | [[Category: Helical fibril]] | ||
| + | [[Category: Ll-37]] | ||
Revision as of 11:56, 23 March 2022
Crystal structure of the human LL37(17-29) I24C mutant antimicrobial peptide
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