7t4d
From Proteopedia
(Difference between revisions)
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==Pore structure of pore-forming toxin Epx4== | ==Pore structure of pore-forming toxin Epx4== | ||
| - | <StructureSection load='7t4d' size='340' side='right'caption='[[7t4d]]' scene=''> | + | <StructureSection load='7t4d' size='340' side='right'caption='[[7t4d]], [[Resolution|resolution]] 3.00Å' scene=''> |
== Structural highlights == | == Structural highlights == | ||
| - | <table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7T4D OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7T4D FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[7t4d]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7T4D OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7T4D FirstGlance]. <br> |
| - | </td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7t4d FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7t4d OCA], [https://pdbe.org/7t4d PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7t4d RCSB], [https://www.ebi.ac.uk/pdbsum/7t4d PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7t4d ProSAT]</span></td></tr> | + | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MPD:(4S)-2-METHYL-2,4-PENTANEDIOL'>MPD</scene></td></tr> |
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7t4d FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7t4d OCA], [https://pdbe.org/7t4d PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7t4d RCSB], [https://www.ebi.ac.uk/pdbsum/7t4d PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7t4d ProSAT]</span></td></tr> | ||
</table> | </table> | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | Enterococci are a part of human microbiota and a leading cause of multidrug resistant infections. Here, we identify a family of Enterococcus pore-forming toxins (Epxs) in E. faecalis, E. faecium, and E. hirae strains isolated across the globe. Structural studies reveal that Epxs form a branch of beta-barrel pore-forming toxins with a beta-barrel protrusion (designated the top domain) sitting atop the cap domain. Through a genome-wide CRISPR-Cas9 screen, we identify human leukocyte antigen class I (HLA-I) complex as a receptor for two members (Epx2 and Epx3), which preferentially recognize human HLA-I and homologous MHC-I of equine, bovine, and porcine, but not murine, origin. Interferon exposure, which stimulates MHC-I expression, sensitizes human cells and intestinal organoids to Epx2 and Epx3 toxicity. Co-culture with Epx2-harboring E. faecium damages human peripheral blood mononuclear cells and intestinal organoids, and this toxicity is neutralized by an Epx2 antibody, demonstrating the toxin-mediated virulence of Epx-carrying Enterococcus. | ||
| + | |||
| + | Emerging enterococcus pore-forming toxins with MHC/HLA-I as receptors.,Xiong X, Tian S, Yang P, Lebreton F, Bao H, Sheng K, Yin L, Chen P, Zhang J, Qi W, Ruan J, Wu H, Chen H, Breault DT, Wu H, Earl AM, Gilmore MS, Abraham J, Dong M Cell. 2022 Mar 2. pii: S0092-8674(22)00142-8. doi: 10.1016/j.cell.2022.02.002. PMID:35259335<ref>PMID:35259335</ref> | ||
| + | |||
| + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | ||
| + | </div> | ||
| + | <div class="pdbe-citations 7t4d" style="background-color:#fffaf0;"></div> | ||
| + | == References == | ||
| + | <references/> | ||
__TOC__ | __TOC__ | ||
</StructureSection> | </StructureSection> | ||
[[Category: Large Structures]] | [[Category: Large Structures]] | ||
| - | [[Category: Abraham J]] | + | [[Category: Abraham, J]] |
| - | [[Category: Dong M]] | + | [[Category: Dong, M]] |
| - | [[Category: Xiong | + | [[Category: Xiong, X Z]] |
| - | [[Category: Yang P]] | + | [[Category: Yang, P]] |
| + | [[Category: Pore-forming toxin]] | ||
| + | [[Category: Toxin]] | ||
Revision as of 12:01, 23 March 2022
Pore structure of pore-forming toxin Epx4
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