2uzj

From Proteopedia

(Difference between revisions)

Revision as of 12:34, 23 March 2022

Crystal structure of the mature streptococcal cysteine protease, mSpeB

Structural highlights

2uzj is a 2 chain structure with sequence from Streptococcus pyogenes. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Ligands:
Activity:	Streptopain, with EC number 3.4.22.10
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

[SPEB_STRP6] Important streptococcal virulence factor which cleaves human fibronectin and degrades vitronectin. Also cleaves human IL1B precursor to form biologically active IL1B. Can induce apoptosis in human monocytes and epithelial cells in vitro, and reduces phagocytic activity in monocytic cells. Thus, may play a role in bacterial colonization, invasion, and inhibition of wound healing.

Evolutionary Conservation

Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.

Publication Abstract from PubMed

Invasive infections of Streptococcus pyogenes are dependent on the cysteine protease streptococcal pyrogenic exotoxin B. Previous structures of the enzyme have not disclosed the proper active-site configuration. Here, the crystal structure of the mature enzyme is presented to 1.55 A, disclosing a homodimer. A serine from one subunit inserts into the active site of the other to donate to the oxyanion hole and coordinates the ligand proximal to the active-site cysteine. Dimerization is unique to the mature form and is clearly a prerequisite for catalysis. The present structure supports a tripartite switch system that is triggered upon dimerization and substrate binding: (1) liberation of the active-site histidine from an inactive configuration, (2) relocation of residues blocking the substrate binding pockets and (3) repositioning of two active-site tryptophans to settle in the active configuration. Based on the present structure, the active site of clan CA cysteine proteases is expanded and a detailed mechanism of the deacylation mechanism is proposed. The results may have applications for the development of protease inhibitors specific to bacterial cysteine proteases.

Structure of the mature Streptococcal cysteine protease exotoxin mSpeB in its active dimeric form.,Olsen JG, Dagil R, Niclasen LM, Sorensen OE, Kragelund BB J Mol Biol. 2009 Oct 30;393(3):693-703. Epub 2009 Aug 25. PMID:19712682^[1]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Olsen JG, Dagil R, Niclasen LM, Sorensen OE, Kragelund BB. Structure of the mature Streptococcal cysteine protease exotoxin mSpeB in its active dimeric form. J Mol Biol. 2009 Oct 30;393(3):693-703. Epub 2009 Aug 25. PMID:19712682 doi:10.1016/j.jmb.2009.08.046

1 Structural highlights
2 Function
3 Evolutionary Conservation
4 Publication Abstract from PubMed
5 See Also
6 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/2uzj"

@@ Line 3: / Line 3: @@
 <StructureSection load='2uzj' size='340' side='right'caption='[[2uzj]], [[Resolution|resolution]] 1.55&Aring;' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[2uzj]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Streptococcus_pyogenes Streptococcus pyogenes]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2UZJ OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=2UZJ FirstGlance]. <br>
+<table><tr><td colspan='2'>[[2uzj]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Streptococcus_pyogenes Streptococcus pyogenes]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2UZJ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2UZJ FirstGlance]. <br>
 </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=E64:N-[N-[1-HYDROXYCARBOXYETHYL-CARBONYL]LEUCYLAMINO-BUTYL]-GUANIDINE'>E64</scene></td></tr>
-<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Streptopain Streptopain], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.4.22.10 3.4.22.10] </span></td></tr>
+<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[https://en.wikipedia.org/wiki/Streptopain Streptopain], with EC number [https://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.4.22.10 3.4.22.10] </span></td></tr>
-<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=2uzj FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2uzj OCA], [http://pdbe.org/2uzj PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2uzj RCSB], [http://www.ebi.ac.uk/pdbsum/2uzj PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=2uzj ProSAT]</span></td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2uzj FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2uzj OCA], [https://pdbe.org/2uzj PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2uzj RCSB], [https://www.ebi.ac.uk/pdbsum/2uzj PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2uzj ProSAT]</span></td></tr>
 </table>
 == Function ==
-[[http://www.uniprot.org/uniprot/SPEB_STRP6 SPEB_STRP6]] Important streptococcal virulence factor which cleaves human fibronectin and degrades vitronectin. Also cleaves human IL1B precursor to form biologically active IL1B. Can induce apoptosis in human monocytes and epithelial cells in vitro, and reduces phagocytic activity in monocytic cells. Thus, may play a role in bacterial colonization, invasion, and inhibition of wound healing.
+[[https://www.uniprot.org/uniprot/SPEB_STRP6 SPEB_STRP6]] Important streptococcal virulence factor which cleaves human fibronectin and degrades vitronectin. Also cleaves human IL1B precursor to form biologically active IL1B. Can induce apoptosis in human monocytes and epithelial cells in vitro, and reduces phagocytic activity in monocytic cells. Thus, may play a role in bacterial colonization, invasion, and inhibition of wound healing.
 == Evolutionary Conservation ==
 [[Image:Consurf_key_small.gif|200px|right]]

2uzj

From Proteopedia

Revision as of 12:34, 23 March 2022

Crystal structure of the mature streptococcal cysteine protease, mSpeB

Structural highlights

Function

Evolutionary Conservation

Publication Abstract from PubMed

See Also

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

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