1e7k
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(New page: 200px<br /> <applet load="1e7k" size="450" color="white" frame="true" align="right" spinBox="true" caption="1e7k, resolution 2.9Å" /> '''CRYSTAL STRUCTURE OF...)
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Revision as of 14:34, 12 November 2007
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CRYSTAL STRUCTURE OF THE SPLICEOSOMAL 15.5KD PROTEIN BOUND TO A U4 SNRNA FRAGMENT
Overview
We have determined the crystal structure of a spliceosomal RNP complex, comprising the 15.5kD protein of the human U4/U6.U5 tri-snRNP and the 5', stem-loop of U4 snRNA. The protein interacts almost exclusively with a, purine-rich (5+2) internal loop within the 5' stem-loop, giving an unusual, RNA fold characterized by two tandem sheared G-A base pairs, a high degree, of purine stacking, and the accommodation of a single RNA base, rotated, out of the RNA chain, in a pocket of the protein. Apart from yielding the, structure of an important entity in the pre-mRNA splicing apparatus, this, work also implies a model for the complex of the 15.5kD protein with box, C/D snoRNAs. It additionally suggests a general recognition principle in a, novel family of RNA binding proteins.
About this Structure
1E7K is a Single protein structure of sequence from Homo sapiens. Full crystallographic information is available from OCA.
Reference
Crystal structure of the spliceosomal 15.5kD protein bound to a U4 snRNA fragment., Vidovic I, Nottrott S, Hartmuth K, Luhrmann R, Ficner R, Mol Cell. 2000 Dec;6(6):1331-42. PMID:11163207
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